DOI QR코드

DOI QR Code

Study on Oxytetracycline Resistant Bacteria in the Surface Water Environment

하천에서의 Oxytetracycline 내성주에 관한 연구

  • Kim, Young Jin (Chung Ryong Environment Co., LTD.) ;
  • Kim, Jong Oh (Department of Environmental Health, Dongnam Health University)
  • Received : 2015.01.21
  • Accepted : 2015.02.14
  • Published : 2015.02.28

Abstract

Objectives: This study aims to understand the concentration, diversity, and antibiotic characteristics of oxytetracycline resistant bacteria present in a surface water environment. Methods: Water sampling was performed in Cheongmi Stream in Gyeonggi-do, Korea in February and August 2014. Water samples collected from two sites were plated in triplicate on tryptic soy agar plates with 30 mg/L of oxytetracycline. Oxytetracycline resistant bacteria were selected from surface water in Cheongmi Stream and were subjected to 16S rDNA analysis for oxytetracycline resistant species determination. Identified resistant strains were tested for resistance to various antibiotics. Results: Results from this study indicate that the dominant resistant organisms in this aquatic environment are from family Acinetobacter and family Aeromonas. As to culturable heterotrophic bacteria, Oxytetracycline resistant bacteria were present 0.45-0.93% during winter and 0.08-0.38% during summer. Most oxytetracycline resistant bacteria exhibited resistance to more than ten of the antibiotics studied. The diversity of oxytetracycline resistant bacteria in winter was higher than in summer. Conclusion: Most of these resistant bacteria are Gram negative and are closely related to pathogenic species. These results suggest that increasing multi-antibiotic resistant bacteria in the surface water environment has a close relation to the reckless use of antibiotics in livestock.

Acknowledgement

Supported by : 동남보건대학교

References

  1. Khachatourians GC. Agricultural use of antibiotics and the evolution and transfer of antibiotic-resistant bacteria. CMAJ. 1998; 159(9): 1129-1136.
  2. Dewey CE, Cox BD, Budh EJ, Hurd HS. Association off-label feed additives and farm size, veterinary consultant use and animal age. Pre Vet Med. 1997; 31(1-2): 133-145. https://doi.org/10.1016/S0167-5877(96)01077-X
  3. Kwon HK, Lee JH, Kim JG. A Study on the distribution of antibiotic resistant bacteria in domesticated animal feces. J Environ Health Sci. 2012; 38(2): 142-150.
  4. Kwon YI, Kim TW, Kim HY, Chang YH, Kwak HS, Woo GJ, et al. Monitoring of antimicrobial resistant bacteria from animal farm environments in Korea. Kor J Microbio Biotechnol. 2007; 35(1): 17-25.
  5. Ministry of Agriculture, Food and Rural Affairs. News. Available. http://www.mafra.go.kr/list.jsp?&newsid=155444846§ion_id=b_sec_1&pageNo=107&year=2014&listcnt=10&board_kind=C&board_skin_id=C3&depth=1&division=B&group_id=3&menu_id=1125&reference=&parent_code=3&popup_yn=&tab_yn [accessed 4 November 2014].
  6. Suzuki S. Tetracycline Resiatance Gene in Asian Aquatic Environment. Available: http://www.terrapub.co.jp/onlineproceedings/ec/03/pdf/BR_03001.pdf [accessed 14 October 2013].
  7. Kim JH. Ecology risk assessment of pharmaceutical residues in Han River. [PhD thesis]. [Seoul]: Yonsei University; 2008.
  8. National Institute of Environmental Research. News. Available. http://www.nier.go.kr/NIER/Egov-MovePageMain.do?linkPage=nier-nf-03&nId=17113&Command=READ[accessed 4 November 2014]
  9. Oh HK, Park JH. Characteristics of antibiotic resistant bacteria in urban sewage and river. Journal of KSEE. 2009; 31(3): 232-239.
  10. Available: http://terms.naver.com/entry.nhn?docId=1146604&cid=40942&categoryId=33192 [accessed 10 November 2014].
  11. National Statistical Information Service. Livestock Statistics Survey. Available. http://kosis.kr/statistics-List/statisticsList_02List.jsp?vwcd=MT_ATITLE01&parmTabId=M_02_01_01#SubCont [accessed 3 September 2013].
  12. Kim SJ, Ogo M, Oh MJ, Suzuki S. Occurrence of tetracycline resistant bacteria and tet(M) gene in seawater from korea coast. Available: http://www.terrapub.co.jp/onlineproceedings/ec/06/pdf/PR644.pdf [accessed 14 October 2013].
  13. Dang H, Ren J, Song L, Sun S, An L. Diverse tetracycline resistant bacteria and resistance genes from coastal waters of jiaozhou bay. Microb Ecol. 2008; 55: 237-246. https://doi.org/10.1007/s00248-007-9271-9
  14. Kim JO, Kim YJ. A study on the concentration and characteristics of methicillin-resistant staphylococci in the indoor air of childcare facilities. J Environ Health Sci. 2013; 39(5): 447-455.
  15. Clinical Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing; 23rd informational supplement. M100-S23. Wayne, PA: Clinical Laboratory Standards Institute; 2013.
  16. Sutter VL, Kwock YY, Finegold SM. Susceptibility of bacteroides fragilis to six antibiotics determined by standardized antimicrobial disc susceptibility testing. Antimicrob Agents Chemother. 1973; 3(2): 188-193. https://doi.org/10.1128/AAC.3.2.188
  17. Finlay JE, Miller LA, Poupard JA. Interpretive criteria for testing susceptibility of staphylococci to mupirocin. Antimicrob Agents Chemother. 1997; 41(5): 1137-1139.
  18. Forsberg C, Ryding SO. Eutrophication parameters and trophic state indices in 30 Swedish waste-receiving lakes. Arch Hydrobiol. 2007; 190: 189-207.
  19. Hiraish A. Respiratory quinone profiles as tools for identifying different bacterial populations in activated sludge. J Gen Appl Microbiol. 1988; 34: 39-56. https://doi.org/10.2323/jgam.34.39
  20. Ash RJ, Mauck B, Morgan M. Antibiotic resistance of gram-negative bacteria in rivers, united states. Emerg Infect Dis. 2002; 8(7): 713-716. https://doi.org/10.3201/eid0807.010264
  21. Levy SB, Marshall B. Antibacterial resistance worldwide. Nat med. 2004; 10(12): 122-129. https://doi.org/10.1038/nm0204-122
  22. Kim YJ. A study on the variation of phytase activity and the diversity of producing microorganisms in the river environment. [PhD thesis]. [Seoul]: Seoul National University; 2002.
  23. Shim SK, Lee YK, Lee YS, Ju NY, Huh NY. Food Sanitation, 5th ed. Seoul: Jinro Press; 2008. p.26.
  24. Available: http://en.wikipedia.org/wiki/Acinetobacter [accessed 3 December 2014].
  25. Janda JM, Abbott SL. The genus aeromonas: taxonomy, pathogenicity, and infection. Clin Microbiol Rev. 2010; 23(1): 35-73. https://doi.org/10.1128/CMR.00039-09
  26. Avalable: http://www.nytimes.com/2014/01/28/science/antibiotics-in-animals-tied-to-risk-of-human-infection.html?ref=science&_r=1 [accessed 14 October 2014].