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Antagonistic and growth promotion potential of endophytic bacteria of mulberry (Morus spp.)

Pratheesh Kumar, Punathil Meethal;Ramesh, Sushma;Thipeswamy, Thipperudraiah;Sivaprasad, Venkadara

  • Received : 2015.08.28
  • Accepted : 2015.12.22
  • Published : 2015.12.31

Abstract

Endophytes provide multifarious benefits such as promotion of plant growth and yield, suppression of phyto-pathogens, phosphate solubilising and fixation nitrogen. A study has been carried out to explore growth promotion and antifungal activities of endophytes of mulberry (Morus spp.). Endophytic bacteria were isolated from mulberry plants and studied their cultural, morphological characters, growth promotion as well as their antifungal activity against Rhizoctonia bataticola and Fusarium oxysporum , two mulberry root rot associated pathogens. Except two isolates, all bacteria were colourless and the colony size of eight isolates was small. The margin of five isolates was irregular and the consistency of three isolates was creamy, six isolates was slimy and one was mucoid. Texture of seven isolates was convex and others were flat. Eight isolates were gram positive and the rest Gram negative, five were cocci and others were bacilli (rod shaped). Four isolates were motile and all were catalase positive and only three isolates were oxidase positive. Spore staining was positive only for two isolates. The growth promotion study showed that there was significant difference in root length and seedling length. The antagonistic effect of the bacterial isolates was tested against R. bataticola showed significant (p <0.05) influence of the bacteria, days after inoculation and their interaction on the inhibition of fungal growth. The isolate En-7 completely inhibited the fungus followed by En-5 (66.67%). The bacterial isolates significantly (p <0.05) inhibited growth of F. oxysporum in PDA. The mean inhibition was higher (70.45%) in case of En-7 followed by En-8 (68.65%) and En-10 (66.44%). The study reveals that some endophytic bacteria associated with mulberry have growth promotion and antifungal activity and could be explored for promotion of mulberry growth and managing root rot disease.

Keywords

endophytic bacteria;antagonistic activity;growth promotion;mulberry;root rot

References

  1. Sikora RA, Pocasangre L, Feldezum A, Niere B, Vu TT, Dababat AA (2008) Mutualistic endophytic fungi and in-planta suppressiveness to plant parasitic nematodes. Biological Control, 46, 15-23. https://doi.org/10.1016/j.biocontrol.2008.02.011
  2. Sturz AV (1995) The role of endophytic bacteria during seed decay and potato tuberization. Plant Soil, 75, 257-263. https://doi.org/10.1007/BF00011362
  3. Sturz AV, Christie BR, Nowak J (2000) Bacterial endophytes: Potential role in developing sustainable systems of crop production. Crit. Rev. Plant Sci. 19, 1-30. https://doi.org/10.1016/S0735-2689(01)80001-0
  4. Thomas P, Upreti R (2014). Testing of bacterial endophytes from non-host sources as potential antagonistic agents against tomato wilt pathogen Ralstonia solanacearum. Adv. Microbiol. 4, 656-666. https://doi.org/10.4236/aim.2014.410071
  5. Tiwari RP, Hoondal GS, Tewari R (2009) Laboratory techniques in Microbiology & Biotechnology, Abhishek publications, Chandigarh, India. pp. 190.
  6. Upreti R, Thomas P (2015) Root-associated bacterial endophytes from Ralstonia solanacearum resistant and susceptible tomato cultivars and their pathogen antagonistic effects, Front Microbiol. 6, 255. https://doi.org/10.3389/fmicb.2015.00255
  7. Vale M, Seldin L, Araújo FF, Lima R (2010) Plant growth promoting rhizobacteria: fundamentals and applications. In: Maheshwari DK (ed). Plant growth and health promoting bacteria, Springer, Berlin, pp 21-43.
  8. Yoshida S, Hiradate S, Tsukamoto T, Hatakeda K, Shirata A (2001) Antimicrobial activity of culture filtrate of Bacillus amyloliquefaciens RC-2 isolated form mulberry leaves. Phytopathology, 91, 181-187. https://doi.org/10.1094/PHYTO.2001.91.2.181
  9. Pirttilä AM, Joensuu P, Pospiech H, Jalonen J, Hohtola A (2004) Bud endophytes of Scots pine produce adenine derivatives and other compounds that affect morphology and mitigate browning of callus cultures. Physiol. Plantarum 121, 305-312. https://doi.org/10.1111/j.0031-9317.2004.00330.x
  10. Pocasangre L, Sikora RA, Vilich V, Schuster RP (2001) Survey of banana endophytic fungi from Central America and screening for biological control of burrowing nematode ( Rhadopholus similis). Acta Horticulturae, 531, 283-290.
  11. Podolich O, Ardanov P, Zaets I, Pirttilä AM, Kozyrovska N (2015) Reviving of the endophytic bacterial community as a putative mechanism of plant resistance. Plant Soil, 388, 367-377. https://doi.org/10.1007/s11104-014-2235-1
  12. Rosenblueth M, Martinez Romero E (2006) Bacterial Endophytes and Their Interactions with hosts, The American Phytopathological Society, 19, 827-837.
  13. Ryu CM, Farag MA, Hu CH, Reddy MS, Wei HX, Pare PW, Kloepper JW (2003) Bacterial volatiles promote growth in Arabidopsis. Proc. Natl. Acad. Sci. U.S.A. 100, 4927-4932. https://doi.org/10.1073/pnas.0730845100
  14. Sessitsch A, Howieson JG, Perret X, Antoun H, Martínez- Romero E (2002). Advances in Rhizobium research, Crit. Rev. Plant Sci. 21, 323-378. https://doi.org/10.1080/0735-260291044278
  15. Sessitsch A, Reiter B, Berg G (2004) Endophytic bacterial communities of field-grown potato plants and their plant- growth-promoting and antagonistic abilities. Can. J. Microbiol. 50, 239-249. https://doi.org/10.1139/w03-118
  16. Sevilla M, Burris RH, Gunapala N, Kennedy C (2001) Comparison of benefit to sugarcane plant growth and 15N2 incorporation following inoculation of sterile plants with Acetobacter diazotrophicus wild-type and Nif mutant strains. Mol. Plant- Microbe Interact. 14, 358-366. https://doi.org/10.1094/MPMI.2001.14.3.358
  17. Hallman J, Sikora R (1995) Influence of Fusarium oxysporum a mutualistic fungal endophyte, on Meloidogyne incognita infection of tomato. Journal of Plant Disease and Protection 101, 475-481.
  18. He R, Wang G, Liu X, et al (2009) Antagonistic bioactivity of an endophytic bacterium isolated from Epimedium brevicornu Maxim. African Journal of Biotechnology 8, 191-195.
  19. Hurek T, Handley LL, Reinhold-Hurek B, Piche Y (2002) Azoarcus grass endophytes contribute fixed nitrogen to the plant in an unculturable state. Mol. Plant-Microbe Interact. 15, 233-242. https://doi.org/10.1094/MPMI.2002.15.3.233
  20. Iniguez AL, Dong Y, Triplett EW (2004) Nitrogen fixation in wheat provided by Klebsiella pneumonia 342. Mol. Plant-Microbe Interact. 17,1078-1085. https://doi.org/10.1094/MPMI.2004.17.10.1078
  21. Kerry BR (2000) Rhizosphere interactions and the exploitation of microbial agents for the biological control of plant-parasitic nematodes. Ann. Rev. Phytopath 38, 423-441. https://doi.org/10.1146/annurev.phyto.38.1.423
  22. Kloepper JW, Rodriguez-Kabana R, Zehnder GW, Murphy J, Sikora E, Fernandez C (1999) Plant root-bacterial interactions in biological control of soilborne diseases and potential extension to systemic and foliar diseases. Australasian Plant Pathology, 28, 27-33 https://doi.org/10.1071/AP99004
  23. McInroy JA, Kloepper JW (1995) Survey of indigenous bacterial endophytes from cotton and sweet corn. Plant Soil. 173, 337-342. https://doi.org/10.1007/BF00011472
  24. Oliveira ALM, Urquiaga S, Baldani JI (2003) Processos e mecanismos envolvidos na influência de microrganismos sobre o crescimento vegetal. Embrapa Agrobiologia Documentos 161, 1-5.
  25. Ping L, Boland W (2004) Signals from the underground: bacterial volatiles promote growth in Arabidopsis. Trends Plant. Sci. 9, 263-269. https://doi.org/10.1016/j.tplants.2004.04.008
  26. Bakker PAHM, Berendsen RL, Doornbos R F, Wintermans PCA, Pieterse CMJ (2013) The rhizosphere revisited: root microbiomics. Front. Plant Sci. 4,165 10.3389/fpls.2013.00165. https://doi.org/10.3389/fpls.2013.00165
  27. Barretti PB, Souza RM, Pozza EA (2008) Bactérias endofíticas como agents promotores do crescimento de plantas de tomateiro e de inibição in vitro de Ralstonia solanacearum. Ciênc Agrotec 32, 731-739 https://doi.org/10.1590/S1413-70542008000300005
  28. Berg G, Hallmann J (2006) Control of plant pathogenic fungi with bacterial endophytes. Microbial Root Endophytes (Schulz BJE, Boyle CJC, Sieber TN, Eds.), pp. 53-69. Springer-Verlag, Berlin.
  29. Berg G, Krechel A, Ditz M, Sikora RA, Ulrich A, Hallmann J (2005) Endophytic and ectophytic potato-associated bacterial communities differ in structure and antagonistic function against plant pathogenic fungi. FEMS (Fed. Eur. Microbiol. Soc.) Microbiol. Ecol. 51, 215-229. https://doi.org/10.1016/j.femsec.2004.08.006
  30. Boyle C, Gotz M, Dammann-Tugend U, Schultz B (2001) Endo- phyte–host interaction III. Local vs. systemic colonization. Symbiosis 31, 259-281.
  31. Compant S, Duffy B, Nowak J, Clement C, Barka EA (2005) Use of plant growth-promoting bacteria for biocontrol of plant diseases: principles, mechanisms of action, and future prospects. Appl. Environ. Microbiol. 71, 4951-4959. https://doi.org/10.1128/AEM.71.9.4951-4959.2005
  32. Conn VM, Walker AR, Franco CMM (2008) Endophytic actinobacteria induce defense pathways in Arabidopsis thaliana. Mol. Plant Microb. Interact. 21, 208-218. https://doi.org/10.1094/MPMI-21-2-0208
  33. Gray EJ, Smith DL (2005) Intracellular and extracellular PGPR: commonalities and distinctions in the plant-bacterium signaling processes. Soil Biol. Biochem. 37, 395-412. https://doi.org/10.1016/j.soilbio.2004.08.030
  34. Bacon CW, White JF (2000) Microbial Endophytes. New York: Marcel Dekker; 2000. pp. 85-117.