Degumming of Antheraea yamamai silkworm cocoon

Shin, Bong-Seob;Jeon, Jong-Young;Kim, Jong-Ho

  • Received : 2015.11.10
  • Accepted : 2015.12.11
  • Published : 2015.12.31


Oak silkworm, Antheraea yamamai (A. yamamai), has been used for clothing and surgical suture and considered as biomaterial due to RGD tripeptide. This paper reported the degumming conditions of A. yamamai using sodium oleate, high pressure and temperature, and sodium carbonate. Degumming ratio of A. yamamai cocoon using sodium oleate was less than 10%. High pressure and temperature treatment induced 30% weight loss of A. yamamai cocoon. The concentration, treatment temperature and time using sodium carbonate was examined and revealed the following conditions for degumming; 5% owf, 60 min at 100℃. The degummed solution was confirmed using UV and FT-IR spectrometer. Our results can be used to handle A. yamamai silkworm cocoon for further application including material processing.


wild silkworm;degumming ratio;sodium carbonate


  1. Shin BS, Jeon JY, Kim JH (2012) Cocoon characteristics of Antheraea pernyi silkworm rearing in Korean oak field. Int J Indust Entomol 25, 205-208.
  2. Wang J, Yi H, Wei Y (2011) Preliminary biocompatibility evaluation of regenerated Antheraea yamamai silk fibroin in vitro. J Wuhan Univ Tech Mater Sci Ed 26, 1044-1048.
  3. Wei Y, Sun D, Yi H, Wang J (2014) Characterization of a PEG-DE crosslinked tubular silk scaffold. Text Res J 84, 959-967.
  4. You R, Xu Y, Liu Y, Li X, Li M (2014) Comparison of the in vitro and in vivo degradations of silk fibroin scaffolds from mulberry and nonmulberry silkworms. Biomed Mater 10, 015003.
  5. Kweon HY, Lee K, Park K, Kang S, Seok Y (2012) Degumming characteristics and color stability of GoldenSilk cocoon. Int J Ind Entomol 24, 1-5.
  6. Kweon HY, Lee KG, Yeo JH, Woo SO, Han SM, Sohn BH, Lee HS, Shin BS (2006) Characterization of hydrolyzed Antheraea yamamai silk fibroin powder. Korean J Seric Sci 48, 11-15.
  7. Kweon HY, Um IC, Park YH (2001) Structure and thermal characteristics of Antheraea pernyi silk fibroin/chitosan blend film. Polym 42, 6651-6656.
  8. Lee KG, Jo YY, Yeo JH, Lee HS, Kim KY, Park KY, Kweon HY (2013) Dissolution of Antheraea pernyi raw silk with calcium nitrate ethanol solution. J Seric Entomol Sci 51, 15-19.
  9. Lee KG, Chung DE, Kim KY, Jo YY, Kim H, Kim S, Kweon HY (2015) General characteristics of Antheraea yamamai silkworm cocoon cultured in Korea. J Seric Entomol Sci 53, 6-11.
  10. Minoura N, Aiba SI, Higuchi M, Gotoh Y, Tsukada M, Imai Y (1995) Attachment and growth of fibroblast cells on silk fibroin. Biochem Biophys Res Commun 208(2), 511-516.
  11. Liu Y, Zhang Y (2014) Control of the gelation process of silk fibroin solution. Thermal Science 18, 1587-1590.
  12. Moy RL, Lee A, Zalka A (1991) Commonly used suture materials in skin surgery. Am Fam Physician 44, 2123-2128.
  13. Oh HJ, Lee JY, Kim MK, Um IC, Lee KH (2011) Refining hot-water extracted silk sericin by ethanol-induced precipitation. Int J Biol Macromol 48, 32-37.
  14. Ruoslahti E, Pierschbacher MD (1986) Arg-Gly-Asp: a versatile cell recognition signal. Cell 44, 517-518.
  15. Datta A, Ghosh AK, Kundu SC (2001) Purification and characterization of fibroin from the tropical saturniid silkworm, Antheraea mylitta. Insect Biochem Mol Biol 31, 1013-1018.
  16. Freddi G, Gotoh Y, Mor T, Tsubsui I, Tsukada M (1994) Chemical structure and physical properties of Antheraea assama silk. J Appl Polym Sci 52, 775-781.
  17. Kawahara Y, Nakajima S (1992) Surface and mechanical properties of wild silk fibers degummed with enzyme. J Seric Sci Jpn 61, 460-462.
  18. Kim JH, Shin BS, Lee KG, Yeo JH, Kweon HY (2009) Sericinjam sericin: structure and thermal properties. Int J Indust Entomol 19, 255-258.
  19. Kwak HW, Yang YS, Kim MK, Lee JY, Yun H, Kim MH, Lee KH (2013) Chromium(VI) adsorption behavior of silk sericin beads. Int J Indust Entomol 26, 47-53.