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Toxicity of Eun-bi San Pharmacopuncture Injection inthe Muscle of Rats

  • Lee, Jongcheol ;
  • Jeong, Hohyun ;
  • Cha, Eunhye ;
  • Park, Manyong ;
  • Choi, Sungyoul ;
  • Yook, Taehan ;
  • Song, InJa ;
  • Kim, Sungchul
  • Received : 2014.10.21
  • Accepted : 2014.11.06
  • Published : 2015.03.30

Abstract

Objectives: This study was carried out to analyze the single-dose toxicity of Eun-Bi San pharmacopuncture injected into the muscle of Sprague-Dawley (SD) rats. Methods: All experiments were performed at Biotoxtech, an institution certified to conduct non-clinical studies under the Good Laboratory Practice (GLP) regulations. Six week old SD rats reared by ORIENTBIO were chosen for this pilot study. The reason SD rats were chosen is that they have been widely used in safety tests in the field of medicine, so the results can be easily compared with many other databases. The Eun-Bi San pharmacopuncture was made in a clean room at the Korean Pharmacopuncture Institute (KPI, K-GMP). The constituents of the Eun-Bi San pharmacopuncture are Angelicae gigantis radix, Strychni semen and Glycyrrhizae radix. These were extracted at low temperature and low pressure in an aseptic room at the KPI. Doses of Eun-Bi San pharmacopuncture, 0.25, 0.5 and 1.0 mL, were administered to the experimental groups, and a dose of normal saline solution, 1.0 mL, was administered to the control group. This study was performed under the approval of the Institutional Animal Ethics Committee of Biotoxtech Co., Ltd. Results: No deaths or abnormalities occurred in any of the four groups. No significant changes in weight, hematological parameters or clinical chemistry between the control group and the experimental groups were observed. To determine if abnormalities existed in any organs and tissues, we used microscopy to examine representative histological sections of each specified organ; the results showed no significant differences in any of the organs or tissues. Conclusion: The results of this study suggest that treatment with Eun-Bi San pharmacopuncture is relatively safe and that its clinical use may be beneficial. Further evaluations and studies on this subject will be needed to provide more concrete evidence in support of these conclusions.

Keywords

eun-bi san;pharmacopuncture;toxicity test

References

  1. Korean Pharmacopuncture Institute. [Pharmacopuncturology principles and clinical applications]. Seoul: Elsevier Korea; 2012. p. 3-4. Korean.
  2. Joo HJ. [Researches on parmacopuncture]. KIOM. 1995;5:193-210. Korean.
  3. Seo BI, Roh SS. [A philological study on poisoning of Arecae semen]. The Journal of Jeahan Oriental Medical Academy. 2012;10(1):119-25. Korean.
  4. Kang TG. Antimicrobial effect and cytotoxicity of Glycyrrhizae radix [master's thesis]. [Gangwon]: Sangji university. 2008
  5. Choi YJ, Roh JD. [Effects of Angelicae gigantis radix pharmacopuncture on nitric oxide and prostaglandin E2 production in macrophage]. J Pharmacopuncture. 2011;14(3):81-90. Korean.
  6. Lee SM, Lim SC, Lee YK, Kim JS. [Effects of Angelica gigantis pharmacopuncture extracts on the elastase activity and DPPH and NO scavenging activities]. The acupuncture. 2011;28(6):35-42. Korean.
  7. Han HS. [Anti-inflammatory effect of Angelicae acutilobae radix water extract on LPS-stimulated mouse macrophages]. Kor J Herbology. 2013;28(6):129-33. Korean. https://doi.org/10.6116/kjh.2013.28.6.129
  8. Bae CW, Kim KH, Hwang HS, Kim YS. [Effects of Strychni semen aqua-acupuncture in rats with arthritis induced by freund's complete adjuvant]. The acupuncture. 2001;18(1);217-25. Korean.
  9. Lee HD, Song CH. [Effect of glycyrrhizae radix pharmacopuncture on PCA and secretion of ${\beta}$-hexosaminidase and cytokines in RBL-2H3 cells]. Korean J Acupunct. 2011;28(3):73-83. Korean.
  10. Bak JP, Son JH, Kim YM, Lee EY, Leem KH, Kim EH. [Suppression of inflammatory macrophage responese by glycyrrhiza uralensis herbal acupuncture extract]. Korean J Acupunct. 2011;28(4):49-58. Korean.
  11. Kim YK. [The principle and test method. Toxicology]. Seoul: Donghwagisul; 1994(3). p. 15-8. Korean.
  12. Kim YG. [Toxicology]. Seoul: Donghwagisul; 1984. p. 15-8. Korean.
  13. Korea Food & Drug Administration. Korea Food & Drug Administration notification [Internet]. Seoul: Korea Food & Drug Administration; 2005 [cited 2013 Mar 1]. Available from: http://lawwizice.wordpress.com/.

Acknowledgement

Supported by : wonkwang university