Quantitative Analysis of the Three Marker Compounds in Sanguisorbae Radix by Processing Method

포제에 따른 지유의 지표성분 함량분석

  • Seo, Chang-Seob (K-herb Research Group, Korea Institute of Oriental Medicine) ;
  • Kim, Jung-Hoon (K-herb Research Group, Korea Institute of Oriental Medicine) ;
  • Shin, Hyeun-Kyoo (K-herb Research Group, Korea Institute of Oriental Medicine) ;
  • Kim, Byoung-Soo (Department of Physiology, College of Korean Medicine, Daejeon University)
  • 서창섭 (한국한의학연구원 K-herb연구단) ;
  • 김정훈 (한국한의학연구원 K-herb연구단) ;
  • 신현규 (한국한의학연구원 K-herb연구단) ;
  • 김병수 (대전대학교 한의과대학)
  • Received : 2015.09.11
  • Accepted : 2015.10.30
  • Published : 2015.12.31

Abstract

In this study, we performed quantitative determination of the three marker compounds such as gallic acid, ellagic acid, and quercetin in the 70% ethanol extracts of non-processed Sanguisorbae Radix and processed Sanguisorbae Radix using a high-performance liquid chromatography coupled with photodiode array detector. The analytical column for separation of the three compounds was used a Gemini $C_{18}$ column ($5{\mu}m$, $4.6{\times}250mm$) by the gradient elution with distilled water and acetonitrile containing 1.0% (v/v) acetic acid as mobile phase. The flow rate and injection volume were $1.0{\mu}L/min$ and $10{\mu}L$. The concentrations of gallic acid, ellagic acid, and quercetin in non-processed Sanguisorbae Radix were 0.25, 0.26, and 0.007%, respectively, while the concentrations of gallic acid, ellagic acid, and quercetin in non-processed Sanguisorbae Radix 0.14-0.55, 0.27-2.03, and 0.001-0.007%, respectively. Among the three components, the amount of the ellagic acid was increased after processing in Sanguisorbae Radix.

Acknowledgement

Supported by : 지식경제부

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