In vitro combination effects of natural substances and antimicrobials against Edwardsiella tarda and Streptococcus iniae

In vitro에서 Edwardsiella tarda와 Streptococcus iniae에 대한 항균제와 천연 유래 항균물질의 병용효과

  • Bak, Su-Jin (Department of Aquatic Life Medicine, Kunsan National University) ;
  • Kang, Bong-Jo (Jeju Special Self-Governing Province Fisheries Resources Research Institute) ;
  • Park, Kwan Ha (Department of Aquatic Life Medicine, Kunsan National University)
  • 박수진 (군산대학교 해양과학대학 수산생명의학과) ;
  • 강봉조 (제주특별자치도 해양수산자원연구소) ;
  • 박관하 (군산대학교 해양과학대학 수산생명의학과)
  • Received : 2014.12.08
  • Accepted : 2015.04.17
  • Published : 2015.04.30


The purpose of this study is to explore the possibility of essential oils and flavonoids improving the performance of antibiotics, proving a higher efficacy in the presence of natural substances against Edwardsiella tarda and Streptococcus iniae. Increased efficacy was observed in several cases: both essential oils and flavonoids increased antibacterial efficacy of oxytetracycline and flumequine against E. tarda by 2-fold; essential oils and flavonoids increased antibacterial efficacy of nalidixic acid against E. tarda by 4-fold; flavonoids increased antibacterial efficacy of josamycin against S. iniae by 4-fold. The results obtained in this study suggest that essential oils and flavonoids might be especially useful in increasing the antimicrobial activity of nalidixic acid and josamycin against E. tarda and S. iniae in fish. Natural compounds examined in this study could also be useful to help decrease the overuse of antibiotics in fish.



  1. Bakkali F., Averbeck S., Averbeck D., and Idaomar M. (2008) Biological effects of essential oils-a review. Food Chem Toxicol, 46(2): 446-475.
  2. Barry A. L., Jones R. N., Thornsberry C., Ayers L. W., Gerlach E. H., and Sommers H. M. (1984) Antibacterial activities of ciprofloxacin, norfloxacin, oxolinic acid, cinoxacin, and nalidixic acid. Antimicrob Agents Chemother, 25(5): 633-637.
  3. Benchaar C., Calsamiglia S., Chaves A. V., Fraser G. R., Colombatto D., McAllister T. A., and Beauchemin K. A. (2008) A review of plant-derived essential oils in ruminant nutrition and production. Anim Feed Sci Technol, 145(1): 209-228.
  4. Burt S. (2004) Essential oils: their antibacterial properties and potential applications in foods-a review. Int J Food Microbiol, 94(3): 223-253.
  5. Cho, M.Y., Kim, M.S., Kwon, M.G., Jee, B.Y., Choi, H.S., Choi, D.L., Park, G.H., Lee, C.H., Kim, J.D., Lee, J.S., Oh, Y.K., Lee, D.C., Park, S.H and Park, M.A. (2007) Epidemiological study of bacterial diseases of cultured olive flounder, Paralichthys olivaceus from 2005 to 2006 in Korea. J. Fish Pathol., 20(1): 61-70.
  6. Choi, W.S., Jung, C.J., Jang, D.Y., Cha, K.M., Um, D. Y., Kim, T.J., and Jung, B.J. (2006) In vitro and in vivo antifunal activaties of derivatives of thymol (I) and carvacrol (II) againt phytopathogenic fungi. The Korean Journal of Pesticide Science, 10(4): 237-248.
  7. Cushnie T. P., and Lamb A. J. (2005) Antimicrobial activity of flavonoids. Int J Antimicrob Agents, 26(5): 343-356.
  8. Hardy D. J., Hensey D. M., Beyer J. M., Vojtko C., McDonald E. J., and Fernandes P. B. (1988) Comparative in vitro activities of new 14-, 15-, and 16- membered macrolides. Antimicrob Agents Chemother, 32(11): 1710-1719.
  9. Hemaiswarya S., and Doble M. (2009) Synergistic interaction of eugenol with antibiotics against Gram negative bacteria. Phytomedicine, 16(11): 997-1005.
  10. Hemaiswarya S., Kruthiventi A. K., and Doble M. (2008) Synergism between natural products and antibiotics against infectious diseases. Phytomedicine, 15(8): 639-652.
  11. Jeong, H.D., and Chun, S.K. (1992) The utilization of antibiotics and the treatment of bacterial diseases in fish. J. Fish Pathol., 5(1): 37-48.
  12. Jung, K.A., and Park, C.S. (2013) Antioxidative and antimicrobial activities of juice from garlic, ginger, and onion. Korean J Food Preserv, 20(1): 134-139.
  13. Kang, S.Y., Kang, J.Y., Kim, S.Y., Kim, D.H., and Oh, M.J. (2008) Antimicrobial activities of alkyl gllates alone and in combination with antibiotics against the fish pathogenic bacteria Edwardsiella tarda and Vibrio anguillarum. Fish Aquat Sci., 41(3): 188-192.
  14. Kim, A.R., Kim, D.K., Byun, T.H., Jo, E.J., Lee, E.W., Kwon, H.J., Kim, B.W., Kim, T.H., Lee, K.B., Kim, Y.M. (2011) Antibacterial activity of herbal medicine extracts against Edwardsiella tarda. Korean J Food Preserv, 18(1): 87-90.
  15. Lambert R. J. W., Skandamis P. N., Coote P. J., and Nychas G. J. (2001) A study of the minimum inhibitory concentration and mode of action of oregano essential oil, thymol and carvacrol. J Appl Microbiol, 91(3): 453-462.
  16. Mirzoeva O. K., Grishanin R. N., and Calder P. C. (1997) Antimicrobial action of propolis and some of its components: the effects on growth, membrane potential and motility of bacteria. Microbiol Res, 152(3): 239-246.
  17. National Fisheries Research and Development Institute (NFRDI): Catalog of aquaculture drugs in 2011, pp 7-62, NFRDI, Ministry of Oceans and Fisheries, 2011.
  18. Ohemeng K. A., Schwender C. F., Fu K. P., and Barrett J. F. (1993) DNA gyrase inhibitory and antibacterial activity of some flavones (1). Bioorg Med Chem Lett, 3(2): 225-230.
  19. Palaniappan K., and Holley R. A. (2010) Use of natural antimicrobials to increase antibiotic susceptibility of drug resistant bacteria. Int J Food Microbiol, 140 (2): 164-168.
  20. Park, K.H., Choo, J.J., and Choe S.N. (2005) Tissue concentrations of querce tin and its metabolite isorhamnetin following oral administration of quercetin in mice. Korean J. Food Sci. Technol., 37(1): 90-94.
  21. Reinhardt J. F., Fowlston S., Jones J., and George W. L. (1985) Comparative in vitro activities of selected antimicrobial agents against Edwardsiella tarda. Antimicrob Agents Chemother, 27(6): 966-967.
  22. Samuelsen O. B. (2006) Pharmacokinetics of quinolones in fish: a review. Aquaculture, 255(1): 55-75.
  23. Thoroski J., Blank G., Biliaderis C. (1989) Eungeol induced inhibition of extracellular enzyme production by Bacillus cereus. J Food Prot, 52(6):399-403.
  24. Wendakoon C.N. and Sakaguchi M. (1995) Inhibition of amino acid decarboxylase activity of Enterobacter aerogenes by active components in spices. J Food Prot, 58(3): 280-283.
  25. Yang, Y.K., Kim, J.Y., and Kwon, O.R. (2012) Development of flavonoid database for commonly consumed foods by Koreans. J Nutr Health, 45(3): 283-292.
  26. You, A.S., Choi, Y.W., Jeong, M.H., Hong, S.S., Park, Y.K., Jang, H.S., Park, J.Y., and Park, K.H. (2011) Acute ecotoxicity evaluation of thyme white, clove bud, cassia, lavender, lemon eucalyptus essential oil of plant extracts. The Korean Journal of Pesticide Science, 15(4): 350-356.

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