Free Radical Scavenging Effect and Oxidative Stress Protective Activity of Domestic Processed Polygoni Multiflori Radix

국내산 법제 하수오의 라디칼 소거능 및 산화적 스트레스 개선 효과

  • Kim, Hyun Young (Department of Food Science, Gyeongnam National University of Science and Technology) ;
  • Kim, Jun Young (Busan Regional Korea Food and Drug Administration, Ministry of Food and Drug Safety) ;
  • Cho, Eun Ju (Department of Food Science and Nutrition, Pusan National University) ;
  • Choi, Ji Myung (Department of Food Science and Nutrition, Pusan National University) ;
  • Hwang, Chung Eun (Department of Food Science, Gyeongnam National University of Science and Technology) ;
  • Lee, Hee Yul (Department of Food Science, Gyeongnam National University of Science and Technology) ;
  • Ahn, Min Ju (Department of Food Science, Gyeongnam National University of Science and Technology) ;
  • Lee, Jin Hwan (National Institute of Chemical Safety, Ministry of Environment) ;
  • Kim, Yun-Geun (Sancheong Oriental Medicinal Herb Institute) ;
  • Ko, Keon Hee (Sancheong Oriental Medicinal Herb Institute) ;
  • Goo, Young-Min (Sancheong Oriental Medicinal Herb Institute) ;
  • Oh, Kyeong Yeol (Sancheong Oriental Medicinal Herb Institute) ;
  • Cho, Kye Man (Department of Food Science, Gyeongnam National University of Science and Technology)
  • 김현영 (경남과학기술대학교 식품과학과) ;
  • 김준영 (식품의약품안전처 부산지방식품의약품안전청) ;
  • 조은주 (부산대학교 식품영양학과) ;
  • 최지명 (부산대학교 식품영양학과) ;
  • 황정은 (경남과학기술대학교 식품과학과) ;
  • 이희율 (경남과학기술대학교 식품과학과) ;
  • 안민주 (경남과학기술대학교 식품과학과) ;
  • 이진환 (환경부 화학물질안전원) ;
  • 김윤근 ((재)산청한방약초연구소) ;
  • 고건희 ((재)산청한방약초연구소) ;
  • 구영민 ((재)산청한방약초연구소) ;
  • 오경렬 ((재)산청한방약초연구소) ;
  • 조계만 (경남과학기술대학교 식품과학과)
  • Received : 2015.04.08
  • Accepted : 2015.05.29
  • Published : 2015.06.30


In this study, we confirmed biological compounds from methanol (MeOH) extract of processed Polygoni Multiflori Radix (PPMR), and the radical scavenging effect and oxidative stress protective activity of MeOH extract of PPMR were investigated under in vitro conditions using LLC-$PK_1$ renal epithelial cells. In HPLC analysis, MeOH extract of PPMR contained four species of biological compounds named 2,3,5,4'-tetrahydroxystilbene 2-O-${\beta}$-D-glucoside, emodin, chrysophanol, and rhein. 2,3,5,4'-Tetrahydroxystilbene 2-O-${\beta}$-D-glucoside was detected as the main compound in PPMR as 115.02 mg/kg. MeOH extract of PPMR showed 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) diammonium salt (ABTS), and hydroxyl radical scavenging activities in a concentration- dependent manner. In particular, upon $50{\mu}g/mL$ of PPMR extract treatment, DPPH, ABTS, and hydroxyl radical scavenging activities were approximately 48.4%, 57.9%, and 81.2%, respectively. LLC-$PK_1$ cell viability declined in response to oxidative stress induced by pyrogallol, sodium nitroprusside (SNP), and morpholinosydnonimine (SIN-1) generators of NO, $O_2{^-}$, and $ONOO^-$, respectively. However, MeOH extract of PPMR significantly and dose-dependently inhibited oxidative-stressed LLC-$PK_1$ cell cytotoxicity. In fact, upon $50{\mu}g/mL$ of PPMR extract treatment, LLC-$PK_1$ cell viabilities were approximately 82.1%, 89.1%, and 77.6% compared to stress levels induced by pyrogallol, SNP, and SIN-1, respectively.


Supported by : 농림축산식품부


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