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Induction of Apoptosis by Gamma-Irradiated Apigenin in H1975 Human Non-Small Lung Cells

감마선 조사된 Apigenin의 H1975 인체 비소폐암세포에서의 Apoptosis 유발 효과

  • Park, Jae-Nam (Advance Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Byun, Eui-Baek (Advance Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kim, Jwa-Jin (Department of Anatomy, College of Medicine, Konyang University) ;
  • Jang, Beon-Su (Advance Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Park, Sang-Hyun (Advance Radiation Technology Institute, Korea Atomic Energy Research Institute)
  • 박재남 (한국원자력연구원 첨단방사선연구소 생명공학연구부) ;
  • 변의백 (한국원자력연구원 첨단방사선연구소 생명공학연구부) ;
  • 김좌진 (건양대학교 의과대학) ;
  • 장범수 (한국원자력연구원 첨단방사선연구소 생명공학연구부) ;
  • 박상현 (한국원자력연구원 첨단방사선연구소 생명공학연구부)
  • Received : 2014.12.17
  • Accepted : 2015.04.21
  • Published : 2015.06.30

Abstract

The objective of this study was to evaluate the anticancer effects of gamma-irradiated apigenin against various human cancer cells. Structural changes were analyzed by high pressure liquid chromatography. Gamma-irradiated apigenin showed a new peak distinguished from the main peak of apigenin (non-irradiated). Cytotoxic effects in human normal cells (HS68) were not observed upon gamma-irradiated and non-irradiated apigenin treatment. However, gamma-irradiated apigenin treatment significantly increased cytotoxicity against non-small lung cancer cells. For apoptosis induction activity tested by Annexin V/PI staining, gamma-irradiated apigenin showed a stronger effect than non-irradiated apigenin, and the level of reactive oxygen species was apparently elevated by gamma-irradiated apigenin treatment. These results suggest that gamma irradiation could be an effective method for development of a new physiological compound from an original compound by inducing structural changes.

Acknowledgement

Supported by : 미래창조과학부

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