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감마선 조사된 Apigenin의 H1975 인체 비소폐암세포에서의 Apoptosis 유발 효과

Induction of Apoptosis by Gamma-Irradiated Apigenin in H1975 Human Non-Small Lung Cells

  • 박재남 (한국원자력연구원 첨단방사선연구소 생명공학연구부) ;
  • 변의백 (한국원자력연구원 첨단방사선연구소 생명공학연구부) ;
  • 김좌진 (건양대학교 의과대학) ;
  • 장범수 (한국원자력연구원 첨단방사선연구소 생명공학연구부) ;
  • 박상현 (한국원자력연구원 첨단방사선연구소 생명공학연구부)
  • Park, Jae-Nam (Advance Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Byun, Eui-Baek (Advance Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kim, Jwa-Jin (Department of Anatomy, College of Medicine, Konyang University) ;
  • Jang, Beon-Su (Advance Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Park, Sang-Hyun (Advance Radiation Technology Institute, Korea Atomic Energy Research Institute)
  • 투고 : 2014.12.17
  • 심사 : 2015.04.21
  • 발행 : 2015.06.30

초록

본 연구는 감마선 조사에 의해 유도된 apigenin 화합물(radiolysis products)이 인체유래 다양한 암세포에 처리했을 때의 항암 효과를 알아보기 위하여 실험을 진행하였다. Apigenin을 50 kGy로 조사할 경우 유도 화합물이 생성됨을 확인할 수 있었으며, radiolysis 화합물을 분획하여 인체유래 섬유육종세포(HS68)에 대한 독성을 평가한 결과 독성이 없는 것으로 나타났다. 다양한 암세포에 항암 활성을 평가한 결과 폐암(H1975)세포주의 경우 다른 암세포에 비해 효과가 높은 것으로 나타났다. Annexin V/PI 염색을 통해 감마선 조사된 apigenin 처리구에서 apoptosis의 발현을 확인할 수 있었으며, ROS(reactive oxygen species) 평가 결과에서도 농도 의존적으로 발현을 확인할 수 있었다. 본 연구는 방사선 구조 변환 연구를 통해 방사선을 이용한 새로운 신약 개발 가능성을 제시하는 기초자료로 활용될 수 있을 것으로 판단하였다.

과제정보

연구 과제 주관 기관 : 미래창조과학부

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피인용 문헌

  1. Gamma irradiation enhanced Tollip-mediated anti-inflammatory action through structural modification of quercetin in lipopolysaccharide-stimulated macrophages vol.42, 2017, https://doi.org/10.1016/j.intimp.2016.11.030