Characteristics and Preparation of Calcium Acetate from Butter Clam (Saxidomus purpuratus) Shell Powder by Response Surface Methodology

반응표면분석법을 이용한 개조개(Saxidomus purpuratus) 패각분말로부터 가용성 초산칼슘의 제조 및 특성

  • Lee, Hyun Ji (Department of Food and Nutrition, Institute of Marine Industry, Gyeongsang National University) ;
  • Jung, Nam Young (Nutrition Education Major, Graduate School of Education, Gyeongsang National University) ;
  • Park, Sung Hwan (Department of Food and Nutrition, Institute of Marine Industry, Gyeongsang National University) ;
  • Song, Sang Mok (Department of Food and Nutrition, Institute of Marine Industry, Gyeongsang National University) ;
  • Kang, Sang In (Department of Seafood Science and Technology, Institute of Marine Industry, Gyeongsang National University) ;
  • Kim, Jin-Soo (Department of Seafood Science and Technology, Institute of Marine Industry, Gyeongsang National University) ;
  • Heu, Min Soo (Department of Food and Nutrition, Institute of Marine Industry, Gyeongsang National University)
  • 이현지 (경상대학교 식품영양학과.해양산업연구소) ;
  • 정남영 (경상대학교 교육대학원 영양교육전공) ;
  • 박성환 (경상대학교 식품영양학과.해양산업연구소) ;
  • 송상목 (경상대학교 식품영양학과.해양산업연구소) ;
  • 강상인 (경상대학교 해양식품공학과.해양산업연구소) ;
  • 김진수 (경상대학교 해양식품공학과.해양산업연구소) ;
  • 허민수 (경상대학교 식품영양학과.해양산업연구소)
  • Received : 2015.02.02
  • Accepted : 2015.03.03
  • Published : 2015.06.30


For effective utilization of butter clam shell as a natural calcium resource, the optimal conditions for preparation of calcium acetate (BCCA) with high solubility were determined using response surface methodology (RSM). The polynomial models developed by RSM for pH, solubility, and yield were highly effective in describing the relationships between factors (P<0.05). Increased molar ratio of calcined powder (BCCP) from butter clam shell led to reduction of solubility, yield, color values, and overall quality. Critical values of multiple response optimization to independent variables were 2.70 M and 1.05 M for acetic acid and BCCP, respectively. The actual values (pH 7.04, 93.0% for solubility and 267.5% for yield) under optimization conditions were similar to predicted values. White indices of BCCAs were in the range of 89.7~93.3. Therefore, color value was improved by calcination and organic acid treatment. Buffering capacity of BCCAs was strong at pH 4.88 to 4.92 upon addition of ~2 mL of 1 N HCl. Calcium content and solubility of BCCAs were 20.7~22.8 g/100 g and 97.2~99.6%, respectively. The patterns of fourier transform infrared spectrometer and X-ray diffractometer analyses from BCCA were identified as calcium acetate monohydrate, and microstructure by field emission scanning electron microscope showed an irregular form.


  1. Ministry of Ocean and Fisheries. 2015. Yearbook of marine resource.¤tPageNo=1 (accessed Jan 2015).
  2. Jeong BY, Choi BD, Moon SK, Lee JS, Jeong WG, Kim PH. 1999. Proximate composition and sterol content of 35 species of marine invertebrates. J Korean Fish Soc 32: 192-197.
  3. Statistics Korea. 2012. Fishery Production Survey. (accessed Jan 2015).
  4. Barros MC, Bello PM, Bao M, Torrado JJ. 2009. From waste to commodity: transforming shells into high purity calcium carbonate. J Cleaner Prod 17: 400-407.
  5. Kasprzak KS, Hoover KL, Poirier LA. 1985. Effects of dietary calcium acetate on lead subacetate carcinogenicity in kidneys of male Sprague-Dawley rats. Carcinogenesis 6:279-282.
  6. d'Almeida Filho EJ, da Cruz EA, Hoette M, Ruzany F, Keen LN, Lugon J. 2000. Calcium acetate versus calcium carbonate in the control of hyperphosphatemia in hemodialysis patients. Sao Paulo Med J 118: 179-184.
  7. Costilow RN, Gerhardt P. 1983. Dialysis pure-culture process for lactic-acid fermentation of vegetables. J Food Sci 48: 1632-1636.
  8. Fleming HP, McDonald LC, McFeeters RF, Thompson RL, Humphries EG. 1995. Fermentation of cucumbers without sodium chloride. J Food Sci 60: 312-315.
  9. Kuhn DF. 2001. Method of treating commercial grade products to remove undesirable odors and flavors. US Patent 6303172 B1.
  10. Okano T, Tsugawa N, Higashino R, Kobayashi T, Igarashi C, Ezawa I. 1991. Effect of bovine bone powder and calcium carbonate as a dietary calcium source on plasma and bone calcium metabolism in rats. J Jpn Soc Nutr Food Sci 44: 479-485.
  11. Han JS, Lee MH, Kim MS, Minamide T. 2000. The study for utilization of pork bone as calcium reinforcement diet. J East Asian Soc Dietary Life 10: 153-159.
  12. Kim JS, Choi JD, Kim DS. 1998. Preparation of calcium based powder from fish bone and its characteristics. Agric Chem Biotechnol 41: 147-152.
  13. Kim JS, Choi JD, Koo JG. 1998. Component characteristics of fish bone as a food source. Agric Chem Biotechnol 41:67-72.
  14. Kim JS, Yang SK, Heu MS. 2000. Component characteristics of cooking tuna bone as a food resource. J Korean Fish Soc 33: 38-42.
  15. Kim JS, Cho ML, Heu MS. 2000. Preparation of calcium powder from cooking skipjack tuna bone and its characteristics. J Korean Fish Soc 33: 158-163.
  16. Cho ML, Heu MS, Kim JS. 2001. Food component characteristics of cuttle bone as a mineral source. J Korean Fish Soc 34: 478-482.
  17. Cho ML, Heu MS, Kim JS. 2001. Study on pretreatment methods for calcium extraction from cuttle bone. J Korean Fish Soc 34: 483-487.
  18. Kim HS, Lee MY, Lee SC. 2000. Characteristics of sepiae os as a calcium source. J Korean Soc Food Sci Nutr 29:743-746.
  19. Lee MJ, Kim HS, Lee SC, Park WP. 2000. Effects of sepiae os addition on the quality of kimchi during fermentation. J Korean Soc Food Sci Nutr 29: 592-596.
  20. Shin HS, Kim KH. 1997. Preparation of calcium powder from eggshell and use of organic acids for enhancement of calcium ionization. Agric Chem Biotechnol 40: 531-535.
  21. Shin HS, Kim KH, Yoon JR. 1998. Rheological properties of cooked noodle fortified with organic acids-eggshell calcium salts. Korean J Food Sci Technol 30: 1197-1202.
  22. Bao SF, Windisch W, Kirchgessner M. 1997. Calcium bioavailability of different organic and inorganic dietary Ca sources (citrate, lactate, acetate, oyster-shell, eggshell, ${\beta}$-tri-Ca phosphate). J Anim Physiol Anim Nutr 78: 154-160.
  23. Kim GH, Jeon YJ, Byun HG, Lee YS, Lee EH, Kim SK. 1998. Effect of calcium compounds from oyster shell bound fish skin gelatin peptide in calcium deficient rats. J Korean Fish Soc 31: 149-159.
  24. Kang JH, Kim JH, Lee HC. 1996. A study on the development of manufacturing process of high grade precipitated calcium carbonate from oyster shell. J Korean Solid Wastes Eng Soc 13: 320-327.
  25. Onoda H, Nakanishi H. 2012. Preparation of calcium phosphate with oyster shells. Nat Resour 3: 71-74.
  26. Mohamed M, Yusup S, Maitra S. 2012. Decomposition study of calcium carbonate in cockle shell. J Eng Sci Technol 7: 1-10.
  27. Okuhira T, Kuwana Y. 1995. Technical in formation update. Characteristics and application of tangle minerals. In Technical J Food Chemistry & Chemicals. Food Chemistry Newspaper Office, Tokyo, Japan. Vol 13, p 112-117.
  28. Anthony JE, Hadgis PN, Milam RS, Herzfeld GA, Taper LJ, Ritchey SJ. 1983. Yields, proximate composition and mineral content of fish shellfish. J Food Sci 48: 313-314.
  29. Kaneko K, Otoguro C, Hihara M, Tsuji K, Odake S, Maeda Y. 1993. Effect of ashed egg shell and ashed oyster shell on hardness, chemical components and tissue structure of brined ume fruit. Nippon Shokuhin Kogyo Gakkaishi 40:577-582.
  30. Kim SD, Ki MK, Kang MS, Lee YK, Kim DS. 2000. Effects of ark shell powder on the fermentation and quality of kimchi. Food Sci Biotechnol 9: 280-284.
  31. Reddy NR, Flick G, Dupuy HP, Boardman GD. 1989. Characterization and utilization of dehydrated wash waters from clam processing plants as flavoring agents. J Food Sci 54:55-59.
  32. Kim JS, Cho ML, Heu MS, Cho TJ, An HJ, Cha YJ. 2003. Solubility improvement of cuttle bone powder using organic acids. J Korean Fish Soc 36: 11-17.
  33. Ko MK, No HK. 2002. Preparation of calcium lactate from ostrich egg shell. J Korean Soc Food Sci Nutr 241-245.
  34. Kang MS, Soh GS, Shin DH. 2005. Purity improvement of calcium lactate and calcium citrate prepared with shell of Anadarac tegillarca granosa. J Fd Hyg Safety 20: 128-133.
  35. Ko MK, No HK. 2002. Studies on characteristics of ostrich egg shell and optimal ashing conditions for preparation of calcium lactate. J Korean Soc Food Sci Nutr 31: 236-240.
  36. Lee MY, Lee YK, Kim SD. 2004. Quality characteristics of calcium acetate prepared with vinegars and ash of black snail. J Korean Soc Food Sci Nutr 33: 593-597.

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