Toxic Effect of Cadmium Assay in Contaminated Soil Earthworm Cell Using Modified Sensor

  • Ly, Suw Young (Biosensor Research Institute, Seoul National University of Science & Technology) ;
  • Kyung, Lee (Biosensor Research Institute, Seoul National University of Science & Technology) ;
  • Kim, Chae Hwa (Biosensor Research Institute, Seoul National University of Science & Technology) ;
  • Seo, Roma (Biosensor Research Institute, Seoul National University of Science & Technology) ;
  • Lee, Soo Youn (Biosensor Research Institute, Seoul National University of Science & Technology) ;
  • Kim, Lina (Biosensor Research Institute, Seoul National University of Science & Technology) ;
  • Chae, Su min (Biosensor Research Institute, Seoul National University of Science & Technology) ;
  • Choi, Sung Wook (Biosensor Research Institute, Seoul National University of Science & Technology) ;
  • Kim, Ji Yoon (Biosensor Research Institute, Seoul National University of Science & Technology)
  • Received : 2015.05.21
  • Accepted : 2015.06.26
  • Published : 2015.06.30


A voltammetric toxic metal of cadmium detection was studied using a fluorine doped graphite pencil electrode (FPE) in a seawater electrolyte. In this study, square wave (SW) stripping and chronoamerometry were used for determination of Cd(II) in seawater. Affordable pencils and an auxiliary electrode were used as reference. All experiments in this study could be performed at reasonable cost by using graphite pencil. The application was performed on the tissue of contaminated soil earthworm. The results show that the method can be applicable for vegetables and in vivo fluid or medicinal diagnosis.


  1. Jarup, L. and Akesson, A. (2009) Current status of cadmium as an environmental health problem. Toxicol. Appl. Pharmacol., 238, 201-208.
  2. Oymak, T., Tokalioglu, S., Yilmaz, V., Kartal, S. and Aydin, D. (2009) Determination of lead and cadmium in food samples by the coprecipitation method. Food Chem., 113, 1314-1317.
  3. Inaba, T., Kobayashi, E., Suwazono, Y., Uetani, M., Oishi, M., Nakagawa, H. and Nogawa, K. (2005) Estimation of cumulative cadmium intake causing Itai-itai disease. Toxicol. Lett., 159, 192-201.
  4. Panayi, A.E., Spyrou, N.M., Iversen, B.S., White, M.A. and Part, P. (2002) Determination of cadmium and zinc in Alzheimer' brain tissue using inductively coupled plasma mass spectrometry. J. Neurol. Sci., 195, 1-10.
  5. Edwards, J.R. and Prozialeck, W.C. (2009) Cadmium, diabetes and chronic kidney disease. Toxicol. Appl. Pharmacol., 238, 289-293.
  6. Il'yasova, D. and Schwartz, G.G. (2005) Cadmium and renal cancer. Toxicol. Appl. Pharmacol., 207, 179-186.
  7. Afkhami, A., Madrakian, T. and Siampour, H. (2006) Flame atomic absorption spectrometric determination of trace quantities of cadmium in water samples after cloud point extraction in Triton X-114 without added chelating agents. J. Hazard. Mater., 138, 269-272.
  8. dos Santos, W.N., Costa, J.L., Araujo, R.G., de Jesus, D.S. and Costa, A.C. (2006) An on-line pre-concentration system for determination of cadmium in drinking water using FAAS. J. Hazard. Mater., 137, 1357-1361.
  9. Silva, E.L., Roldan Pdos, S. and Gine, M.F. (2009) Simultaneous preconcentration of copper, zinc, cadmium, and nickel in water samples by cloud point extraction using 4-(2-pyridylazo)-resorcinol and their determination by inductively coupled plasma optic emission spectrometry. J. Hazard. Mater., 171, 1133-1138.
  10. Robles, L.C. and Aller, A.J. (1995) Determination of cadmium in biological and environmental samples by slurry electrothermal atomic absorption spectrometry. Talanta, 42, 1731-1744.
  11. da Costa, A.B., de Mattos, J.C.P., Muller, E.I., Paniz, J.N.G., Dressler, V.L. and de Moraes Flores, E.M. (2005) Use of paper capsules for cadmium determination in biological samples by solid sampling flame atomic absorption spectrometry. Spectrochim. Acta Part B, 60, 583-588.
  12. Sanchez-Pedreno, C., Garcia, M.S., Ortuno, J.A., Albero, M.I. and Exposito, R. (2002) Kinetic methods for the determination of cadmium(II) based on a flow-through bulk optode. Talanta, 56, 481-489.
  13. Portugal, L.A., Ferreira, H.S., dos Santos, W.N.L. and Ferreira, S.L.C. (2007) Simultaneous pre-concentration procedure for the determination of cadmium and lead in drinking water employing sequential multi-element flame atomic absorption spectrometry. Microchem. J., 87, 77-80.
  14. Lampugnani, L., Salvetti, C. and Tsalev, D.L. (2003) Hydride generation atomic absorption spectrometry with different flow systems and in-atomizer trapping for determination of cadmium in water and urine overview of existing data on cadmium vapour generation and evaluation of critical parameters. Talanta, 61, 683-698.
  15. Talio, M.C., Luconi, M.O., Masi, A.N. and Fernandez, L.P. (2009) Determination of cadmium at ultra-trace levels by CPE-molecular fluorescence combined methodology. J. Hazard. Mater., 170, 272-277.
  16. Aleixo, P.C., Junior, D.S., Tomazelli, A.C., Rufini, I.A., Berndt, H. and Krug, F.J. (2004) Cadmium and lead determination in foods by beam injection flame furnace atomic absorption spectrometry after ultrasound-assisted sample preparation. Anal. Chim. Acta, 512, 329-337.
  17. Hernandez-Caraballo, E.A., Burguera, M. and Burguera, J.L. (2004) Determination of cadmium in urine specimens by graphite furnace atomic absorption spectrometry using a fast atomization program. Talanta, 63, 419-424.
  18. Coco, F.L., Monotti, P., Cozzi, F. and Adami, G. (2006) Determination of cadmium and lead in fruit juices by stripping chronopotentiometry and comparison of two sample pretreatment procedures. Food Control, 17, 966-970.
  19. Hu, Q., Yang, G., Yin, J. and Yao, Y. (2002) Determination of trace lead, cadmium and mercury by on-line column enrichment followed by RP-HPLC as metal-tetra-(4-bromophenyl)-porphyrin chelates. Talanta, 57, 751-756.
  20. Skrzydlewska, E., Balcerzak, M. and Vanhaecke, F. (2003) Determination of chromium, cadmium and lead in food-packaging materials by axial inductively coupled plasma time-of-flight mass spectrometry. Anal. Chim. Acta, 479, 191-202.
  21. Shams, E. and Torabi, R. (2006) Determination of nanomolar concentrations of cadmium by anodic-stripping voltammetry at a carbon paste electrode modified with zirconium phosphated amorphous silica. Sens. Actuators B, 117, 86-92.
  22. Marino, G., Bergamini, M.F., Teixeira, M.F. and Cavalheiro, E.T. (2003) Evaluation of a carbon paste electrode modified with organofunctionalized amorphous silica in the cadmium determination in a differential pulse anodic stripping voltammetric procedure. Talanta, 59, 1021-1028.
  23. Ly, S.Y., Yoo, H.S. and Chun, S.K. (2013) Detection of trace metal in distilled alcoholic drinks. Food Chem., 137, 168-171.
  24. Ly, S.Y., Choi, D.W. and Kim, D.H. (2013) Diagnostic assay of glucose in diabetes patients' urine. Sens. Lett., 11, 1996-1999.
  25. Ensafi, A.A., Khayamian, T., Benvidi, A. and Mirmomtaz, E. (2006) Simultaneous determination of copper, lead and cadmium by cathodic dsorptive stripping voltammetry using artificial neural network. Anal. Chim. Acta, 561, 225-232.
  26. Ly, S.Y. and Choi, D.W. (2013) Implementation of a biocircuit implants for neurotransmitter release during neuro-stimulation. Curr. Neurovasc. Res., 10, 238-246.

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