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Antioxidant activities of blueberry hot water extracts with different extraction condition

추출조건에 따른 블루베리 열수추출물의 항산화 활성 비교

  • Ko, Gyeong-A (Faculty of Biotechnology, College of Applied Life Sciences, SARI, Jeju National University) ;
  • Son, Moa (School of Biomaterials Science and Technology, College of Applied Life Sciences, SARI, Jeju National University) ;
  • Kang, Hye Rim (Faculty of Advanced Convergence Technology and Science, Jeju National University) ;
  • Lim, Ji Hee (JeKiss Co., Ltd. Research Institute) ;
  • Im, Geun Hyung (JeKiss Co., Ltd. Research Institute) ;
  • Kim, Somi (Faculty of Biotechnology, College of Applied Life Sciences, SARI, Jeju National University)
  • Received : 2015.02.16
  • Accepted : 2015.05.26
  • Published : 2015.06.30

Abstract

Five extraction conditions (AE, autoclave extraction; OE, oven extraction; HWSE, hot water and sonication extraction; HWASE, hot water acidified with 0.5% (v/v) acetic acid and sonication extraction; and BE, boiling extraction) were examined to compare the effects of different hot water extraction methods on the antioxidant properties of blueberries. The extraction yields of the AE, OE, HWSE, HWASE, and BE were 7.94%, 8.35%, 8.55% 9.15%, and 8.50%, respectively. The polyphenol and flavonoid contents of AE were 3.47 mg GAE/g and 1.59 mg RE/g, respectively, which were highest centents among others. Those of OE were ranked second to the highest. The total anthocyanin content of HWSE (5.29 mg/g) was significantly higher than that of others whereas that of AE showed the lowest content (0.96 mg/g). The order of ABTS radical and alkyl radical scavenging activities was as follows: AE > BE > OE > HWSE > HWASE. The antioxidant properties were considerably correspondent with the total polyphenol and flavonoid content. DPPH radical scavenging activity was quite high in HWSE, AE, and BE extraction, however, there were no significant differences among the five extraction methods in the aspect of $Fe^{2+}$ ion chelating activities. Moreover, AE showed the highest SOD activity, and protected the dermal fibroblast the best against $H_2O_2$-induced cytotoxicity. In conclusion, it was suggested that the autoclave extraction (AE) would be the most effective method for preparing blueberry hot water extracts with relatively high antioxidant activities.

Acknowledgement

Supported by : 산업통상자원부, 한국산업기술진흥원

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