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Ameliorate Effect of Black Ginseng on HepG2 Cell transplanted in BALB/c Nude Mice

HepG2 세포주를 이식한 BALB/c Nude Mice에서 흑삼의 고형암 개선효과

  • Kang, Shin-Jyung (Dept. of Oriental Pharmaceutical Science, Joongbu University) ;
  • Han, Jung-Soon (Dept. of Alternative Medicine, Kyonggi University) ;
  • Kim, Ae-Jung (Dept. of Alternative Medicine, Kyonggi University)
  • 강신정 (중부대학교 한방제약과학과) ;
  • 한정순 (경기대학교 대체의학대학원) ;
  • 김애정 (경기대학교 대체의학대학원)
  • Received : 2015.01.29
  • Accepted : 2015.04.08
  • Published : 2015.04.30

Abstract

The aim of this study was to examine the ameliorating effect of black ginseng on the growth of the HepG2 cell transplanted tumor in BALB/c nude mice. 27 male BALB/c nude mice (all six weeks old) were randomly divided into three groups: the control group, the first treatment group (HepG2300RG, using 300 mg/kg red ginseng), and the second treatment group (HepG2300BG, using 300 mg/kg black ginseng). The HepG2300BG in the HePG2 cells showed increased mean survival time than that of red ginseng group. The size and volume of the tumor in the 300BG group showed significant reduction compared to those of the HepG2300RG group (p<0.05). The body weight and liver weight of the HepG2300RG group was not significantly different with control and HepG2300BG group. The serum levels of ALT and AST in the HepG2300RG and HepG2300BG group were significantly lower than those of the control group. In conclusion, these results suggest that the black ginseng may have possible anti-tumor activities.

References

  1. Cha JY, Jun BS and Cho YS. 2003. Effect of Korean red ginseng powder on the lipid concentrations and tissues lipid peroxidation in therats fed high fat diet. J Korean Soc Food Sc Nutr 32:124-130 https://doi.org/10.3746/jkfn.2003.32.1.124
  2. Ding FT, Dhavalkumar NPl, Eric CY, Chan AT, Soe KY. Neo HK. 2011. Anti-proliferative effects of raw and steamed extracts of Panax ginseng and its ginsenoside constituents on human liver cancer cells. Chin Med 6:4-10 https://doi.org/10.1186/1749-8546-6-4
  3. Jain RK. 2005. Normalization of tumor vasculature: an emerging concept in antiangiogenic therapy. Science 307:58-62 https://doi.org/10.1126/science.1104819
  4. Jeong YH 2010. Study on immunomodulating and anti-diabetic activity of the extract of black ginseng. MS thesis, Konyang Univ. Nonsan. Korea
  5. Jung SH, Woo MS, Kim SY, Kim WK, Hyun JW, Kim EJ, Kim DH, Kim HS. 2006. Ginseng saponin metabolite suppresses phorbol ster-induced matrix metalloproteinase-9 expression through inhibition of activator protein-1 and mitogen-activated protein kinase signaling pathways in human astroglioma cells. Int J Cancer 118:490-497 https://doi.org/10.1002/ijc.21356
  6. KCCR. 2014. Ministry of Health & Welfare. Annual Report of Cancer Statistics in Korea in 2012, Korean Central Cancer Registry, Ministry of Health & Welfare
  7. Kenarova B, Neychev H, Hadjiivanova C, Petkov VD. 1990. Immunomodulating activity of ginsenoside ${Rg_1}$ from Panax ginseng. Jpn Phamacol 54:447-454
  8. Kim EK, Lee JH, Cho SH, Shen GN, Jin LG, Myung CS, Oh HJ, Kim DH, Yun JD, Roh SS, Park YJ, Seo YB, Song GY 2008. Preparation of black Panax ginseng by new methods and its antitumor activity. Kor J Herbology 23:85-92
  9. Kim EY, Baik IH, Kim JH, Kim SR, Rhyu MR. 2004. Screening of the antioxidant activity of some medicinalplants. Korean J Food Sci Technol 36:333-338
  10. Kim HM. 2006. Effects of Panax ginseng intake to diethylnitrosamine induced rat hepatocarcinogenesis. Ph.D. Thesis, Seoul National Univ. Seoul. Korea
  11. Kim SH. 1999. Studies on protective effect of Panax ginseng on 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) induced acute toxicity in male guinea pigs. MS thesis, Chungnam National Univ. Daejeon. Korea
  12. Kim SM. 2010. Ginsenoside ${Rg_3}$enhances the susceptibility of colon and prostate cancer cells to docetaxel. Ph.D. Thesis, Chungbuk National Univ. Cheongju. Korea
  13. Kim WY, Kim JM, Han SB, Lee SK, Kim ND, Park MK, Kim CK, Park JH 2000. Steaming of ginseng at high temperature enhances biological activity. J Nat Prod 63:1702-1704 https://doi.org/10.1021/np990152b
  14. Kim YK, Guo Q, Packer L. 2002. Free radical scavenging activity of red ginseng aqueous extracts. Toxicology 172:149-156 https://doi.org/10.1016/S0300-483X(01)00585-6
  15. Lee CK, Kim NY, Han YN, Choi JW. 2003b. Effects of pretreated Korean red ginseng on carbontetrachloride and galactosamine-induced hepatotoxicity in rats. J Ginseng Res 27:1-10 https://doi.org/10.5142/JGR.2003.27.1.001
  16. Mochizuki M, Yoo CY, Matsuzawa K, Sato K, Saiki I, Tono-oka S, Samukawa K, Azuma I. 1995. Inhibitory effect of tumor metastasis in mice by saponins, ginsenoside-${Rb_2}$, 20(R)- and 20(S)-ginsenoside-Rg3, of red ginseng. Biol Pharm Bull 18:1197-1202 https://doi.org/10.1248/bpb.18.1197
  17. Namba T, Yoshizaki M, Tomimori T, Kobshi K, Mitsui K. 1974. Chemical and biochemical evaluation of ginseng and related crude drugs. Yakugaku Zasshi 94:252-260
  18. Park SC. 2010. Silibinin enhances the growth inhibitory effects of EGFR-TKIs or sorafenib in hepatocellular carcinoma. Ph.D. Thesis, Kangwon National Univ. Kangwon. Korea
  19. Ponder BAJ. 2001. Cancer genetics. Nature 411:336-341 https://doi.org/10.1038/35077207
  20. Singhal A, Jayaraman M, Dhanasekaran DN, Kohli V. 2012. Molecular and serum markers in hepatocellular carcinoma: Predictive tools for prognosis and recurrence. Critical Reviews in Oncology/Hematology 82:116-140 https://doi.org/10.1016/j.critrevonc.2011.05.005
  21. Son SH. 2010. Inhibition by the black ginseng extract on the growth of HepG2 cells in xenograft tumor regression model. MS Thesis, Chungbuk National Univ. Cheongju. Korea.
  22. Sun BS, Gu LJ, Fang ZM, Wang CY, Wang Z, Sung CK. 2009. Determination of 11 ginsenosides in black ginseng developed from Panax ginseng by high performance liquid chromatography. Food Science and Biotechnology 18:561-564
  23. Wakabayashi C, Hasegawa H, Murata J, Saiki I. 1998. In vivo antimetastatic action of ginseng protopanaxadiol saponins is based on their intestinal bacterial metabolites after oral administration. Oncol Res 9:411-417
  24. Yue PY, Mak NK, Cheng YK, Leung KW, Ng TB, Fan DT, Yeung HW, Wong RN. 2007. Pharmacogenomics and the Yin/Yang actions of ginseng: Anti-tumor, angiomodulating and steroid-like activities of ginsenosides. Clin Med 2:6
  25. Yue PY, Wong DY, Wu PK, Leung PY, Mak NK, Yeung HW, Liu L, Cai Z, Jiang ZH, Fan TP, Wong RN. 2006. The angiosuppressive effects of 20(R)-ginsenoside ${Rg_3}$. Biochem Pharmacol 72:437-445 https://doi.org/10.1016/j.bcp.2006.04.034