DOI QR코드

DOI QR Code

Physiological activities of Agrimonia pilosa extract

짚신나물(Agrimonia pilosa) 추출물의 생리활성

  • Kim, Hyun-Soo (Department of Food Science and Technology, Jungwon University)
  • 김현수 (중원대학교 식품공학과)
  • Received : 2015.01.22
  • Accepted : 2015.03.11
  • Published : 2015.04.30

Abstract

In this study, we investigated the applicability of functional materials by examining various physiological activities with an extract from the Agrimonia pilosa root. The A. pilosa extract showed low cytotoxicity against murine melanoma B16F10 cells. With little or no cytotoxicity at various concentrations, the A. pilosa extract showed high levels of DPPH radical scavenging activity ($ID_{50}$, 20.70 mg/L) and anti-microbial activity against Bacillus subtilis, Escherichia coli, and Candida albicans. In particular, it had a high level of anti-microbial activities against Gram-positive bacteria. These results suggest that the A. pilosa extract can be used as a natural preservative. It also showed inhibition of tyrosinase activity ($ID_{50}$, 90.18 mg/L), as does kojic acid ($ID_{50}$, 89.13 mg/L), and especially, a higher decrease in melanin content ($ID_{50}$, 62.5 mg/L) than the arbutin level ($ID_{50}$, 100.7 mg/L) as a positive control. These findings suggest that the A. pilosa extract inhibits melanin synthesis by suppressing the intracellular tyrosinase expression. These results indicate that the A. pilosa extract may be an effective material for functional cosmetics, such as skin whitening materials.

Acknowledgement

Supported by : 한국산업기술진흥원, 국립원예특작과학원

References

  1. Hsu MF, Young JH, Wang JP, Teng CM (1987) Effect of hsien-ho-t'sao (Agrimonia pilosa) on experimental thrombosis in mice. Am J Chin Med, 15, 43-51 https://doi.org/10.1142/S0192415X87000060
  2. Zhu L, Tan J, Wang B, He R, Liu Y, Zheng C (2009) Antioxidant activities of aqueous extract from Agrimonia pilosa Ledeb and its fractions. Chem Biodivers, 6, 1716-1726 https://doi.org/10.1002/cbdv.200800248
  3. Kato H, Li W, Koike M, Wang Y, Koike K (2010) Phenolic glycosides from Agrimonia pilosa. Phytochem, 71, 1925-1929 https://doi.org/10.1016/j.phytochem.2010.08.007
  4. Koshiura R, Miyamoto K, Ikeya Y, Taguchi H (1985) Antitumor activity of methanol extract from roots of Agrimonia pilosa Ledeb. Japan J Pharmacol, 38, 9-16 https://doi.org/10.1254/jjp.38.9
  5. Shin WJ, Lee KH, Park MH, Seong BL (2010) Broad-spectrum antiviral effect of Agrimonia pilosa extract on influenza viruses. Microbiol Immunol, 54, 11-19 https://doi.org/10.1111/j.1348-0421.2009.00173.x
  6. Yamaki M, Kashihara M, Ishiguro K, Takagi S (1989) Antimicrobial principles of Xian he cao (Agrimonia pilosa). Planta Med, 55, 169-170 https://doi.org/10.1055/s-2006-961915
  7. Jung CH, Zhou S, Ding GX, Kim JH, Hong MH, Shin YC, Kim GJ, Ko SG (2006) Anti-hyperglycemic activity of herb extracts on streptozotocin-induced diabetic rats. Biosci Biotechnol Biochem, 70, 2556-2559 https://doi.org/10.1271/bbb.60238
  8. Jung CH, Kim JH, Park S, Kweon DH, Kim SH, Ko SG (2010) Inhibitory effect of Agrimonia pilosa Ledeb. on inflammation by suppression of iNOS and ROS production. Immunol Invest, 39, 159-170 https://doi.org/10.3109/08820130903501790
  9. Sohn ES, Kim SW, Kang JS, Lee SP (2004) Technology trend patent information analysis of cosmetic materials derived from natural products. Appl Chem, 8, 466-469
  10. Hill HZ, Li W, Xin P, Michell DL (1997) Melanin : a two edged sword ? Pigment Cell Res, 10, 158-161 https://doi.org/10.1111/j.1600-0749.1997.tb00478.x
  11. Kobayashi T, Urabe K, Winder AJ, Jimenez-Cervantes C, Imokawa G, Brewington T, Solano F, Garcia-Borron JC, Hearing VJ (1994) Tyrosinase related protein-1(TRP-1) function as a DHICA oxidase in melanin biosynthesis. EMBO J, 3, 5818-5825
  12. Briganti S, Camera E, Picardo M (2003) Chemical and instrumental approaches to treat hyperpigmentation. Pigment Cell Res, 16, 101-110 https://doi.org/10.1034/j.1600-0749.2003.00029.x
  13. Ranke J, Molter K, Stock F, Bottin-Weber U, Poczobutt J, Hoffmann J, Ondruschka B, Filser J, Jastorff B (2004) Biological effects of imidazolium ionic liquids with varying chain lengths in acute Vibrio fischeri and WST-1 cell viability assays. Ecotoxicol Environ Saf, 58, 396-404 https://doi.org/10.1016/S0147-6513(03)00105-2
  14. Miliauskas G, Venskutonis PR, van Beek TA (2004) Screening of radical scavenging activity of some medicinal and aromatic plant extracts. Food Chem, 85, 231-237 https://doi.org/10.1016/j.foodchem.2003.05.007
  15. Farag RS (1989) Antimicrobial activity of some Egyptian spice essential oils. J Food Prot, 52, 665-670
  16. Masamoto Y, Ando H, Murata Y, Shimoishi Y, Tada M, Takahata K (2003) Mushroom tyrosinase inhibitory activity of esculetin isolated from seeds of Euphorbia lathyris L.. Biosci Biotechnol Biochem, 67, 631-634 https://doi.org/10.1271/bbb.67.631
  17. Hosoi J, Abe E, Suda T, Kuroki T (1985) Regulation of melanin synthesis of B16 mouse melanoma cells by 1 alpha, 25-dihydroxyvitamin D3 and retinoic acid. Cancer Res, 45, 1474-1478
  18. Yoon HS, Yang KW, Kim JE, Kim JM, Lee NH, Hyun CG (2014) Hypopigmenting effects of extracts from bulbs of Lilium Oriental Hybrid 'Siberia' in murine B16/F10 melanoma cells. J Korean Soc Food Sci Nutr, 43, 705-711 https://doi.org/10.3746/jkfn.2014.43.5.705
  19. Jin KS, Lee JY, Kwon HJ, Kim BW (2013) Anti-oxidative, anti-inflammatory, and anti-melanogenic activities of Endlicheria anomala extract. Korean J Microbiol Biotechnol, 41, 433-441 https://doi.org/10.4014/kjmb.1309.09005