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Antioxidant and α-glucosidase inhibitory effects of ethanolic extract of Ainsliaea acerifolia and organic solvent-soluble fractions

단풍취 추출물 및 분획물의 항산화 및 α-glucosidase 저해 활성 평가

  • Lee, Eun-Woo (Department of Life Science and Biotechnology, Dongeui University) ;
  • Kim, Taewan (Department of Food Science and Biotechnology, Andong National University) ;
  • Kim, Hyun-Seok (Department of Food Science and Biotechnology, Andong National University) ;
  • Park, Youn-Moon (Department of Food Science and Biotechnology, Andong National University) ;
  • Kim, Seong-Ho (Department of Food Science and Biotechnology, Daegu University) ;
  • Im, Moo-Hyeog (Department of Food Science and Biotechnology, Daegu University) ;
  • Kwak, Jae Hoon (Faculty of Biotechnology Convergence, Daegu Haany University) ;
  • Kim, Tae Hoon (Department of Food Science and Biotechnology, Daegu University)
  • 이은우 (동의대학교 생명응용학과) ;
  • 김태완 (안동대학교 식품생명공학과) ;
  • 김현석 (안동대학교 식품생명공학과) ;
  • 박윤문 (안동대학교 식품생명공학과) ;
  • 김성호 (대구대학교 식품공학과) ;
  • 임무혁 (대구대학교 식품공학과) ;
  • 곽재훈 (대구한의대학교 바이오산업융합학부) ;
  • 김태훈 (대구대학교 식품공학과)
  • Received : 2014.09.05
  • Accepted : 2014.12.05
  • Published : 2015.04.30

Abstract

Among the naturally occurring antioxidants, polyphenols are widely distributed in various fruits, vegetables, wines, juices, and plant-based dietary sources and divided into several subclasses that included phenolic acid, flavonoids, stilbenes, and lignans. As part of our continuing search for bioactive food ingredients, the antioxidant and ${\alpha}$-glucosidase inhibitory activities of the aqueous ethanolic extract from the aerial parts of Ainsliaea acerifolia were investigated in vitro. The antioxidant properties were evaluated via radical scavenging assays using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) ($ABTS^+$) radicals. In addition, the anti-diabetic effect of A. acerifolia extracts was tested via ${\alpha}$-glucosidase inhibitory assay. Furthermore, the total phenolic contents were determined using a spectrophotometric method. All the tested samples showed dose-dependent radical scavenging and ${\alpha}$-glucosidase inhibitory activities. In particularly, the ${\alpha}$-glucosidase inhibitory and radical scavenging properties of the ethyl acetate (EtOAc)-soluble portion from the aerial parts of the A. acerifolia were higher than those of the other solvent-soluble portions. These results suggest that A. acerifolia could be considered a new potential source of natural antioxidants and antidiabetic ingredients. More systematic investigation of the aerial parts of A. acerifolia will be performed for the further development of anti-oxidative and antidiabetic drugs.

Acknowledgement

Supported by : 산림청, 동의대학교

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