Antioxidant and α-glucosidase inhibitory effects of ethanolic extract of Ainsliaea acerifolia and organic solvent-soluble fractions

단풍취 추출물 및 분획물의 항산화 및 α-glucosidase 저해 활성 평가

  • Lee, Eun-Woo (Department of Life Science and Biotechnology, Dongeui University) ;
  • Kim, Taewan (Department of Food Science and Biotechnology, Andong National University) ;
  • Kim, Hyun-Seok (Department of Food Science and Biotechnology, Andong National University) ;
  • Park, Youn-Moon (Department of Food Science and Biotechnology, Andong National University) ;
  • Kim, Seong-Ho (Department of Food Science and Biotechnology, Daegu University) ;
  • Im, Moo-Hyeog (Department of Food Science and Biotechnology, Daegu University) ;
  • Kwak, Jae Hoon (Faculty of Biotechnology Convergence, Daegu Haany University) ;
  • Kim, Tae Hoon (Department of Food Science and Biotechnology, Daegu University)
  • 이은우 (동의대학교 생명응용학과) ;
  • 김태완 (안동대학교 식품생명공학과) ;
  • 김현석 (안동대학교 식품생명공학과) ;
  • 박윤문 (안동대학교 식품생명공학과) ;
  • 김성호 (대구대학교 식품공학과) ;
  • 임무혁 (대구대학교 식품공학과) ;
  • 곽재훈 (대구한의대학교 바이오산업융합학부) ;
  • 김태훈 (대구대학교 식품공학과)
  • Received : 2014.09.05
  • Accepted : 2014.12.05
  • Published : 2015.04.30


Among the naturally occurring antioxidants, polyphenols are widely distributed in various fruits, vegetables, wines, juices, and plant-based dietary sources and divided into several subclasses that included phenolic acid, flavonoids, stilbenes, and lignans. As part of our continuing search for bioactive food ingredients, the antioxidant and ${\alpha}$-glucosidase inhibitory activities of the aqueous ethanolic extract from the aerial parts of Ainsliaea acerifolia were investigated in vitro. The antioxidant properties were evaluated via radical scavenging assays using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) ($ABTS^+$) radicals. In addition, the anti-diabetic effect of A. acerifolia extracts was tested via ${\alpha}$-glucosidase inhibitory assay. Furthermore, the total phenolic contents were determined using a spectrophotometric method. All the tested samples showed dose-dependent radical scavenging and ${\alpha}$-glucosidase inhibitory activities. In particularly, the ${\alpha}$-glucosidase inhibitory and radical scavenging properties of the ethyl acetate (EtOAc)-soluble portion from the aerial parts of the A. acerifolia were higher than those of the other solvent-soluble portions. These results suggest that A. acerifolia could be considered a new potential source of natural antioxidants and antidiabetic ingredients. More systematic investigation of the aerial parts of A. acerifolia will be performed for the further development of anti-oxidative and antidiabetic drugs.


Supported by : 산림청, 동의대학교


  1. Videla LA, Fermandez V (1988) Biochemical aspects of cellular oxidative stress. Arch Biol Med Exp, 21, 85-92
  2. Halliwell B, Aruoma OJ (1991) DNA damage by oxygen-derived species. FEBS Lett, 281, 9-19
  3. Jennings PE, Barnett AH (1988) New approaches to the pathogenesis and treatment of diabetic microangiopathy. Diabetic Med, 5, 111-117
  4. Shim JS, Kim SD, Kim TS, Kim KN (2005) Biological activities of flavonoid glycosides isolated from Angelica keiskei. Korean J Food Sci Technol, 37, 78-83
  5. Farag RS, Badei AZMA, Hewedi FM, El-Baroty GSA (1989) Antioxidant activity of some spice essential oils on linoleic acid oxidation in aqueous media. J American Oil Chem Soc, 66, 792-799
  6. Frei B (1994) National antioxidants in human health and disease, Academic Press, San Diego, p 44-55
  7. Branen AL (1975) Toxicology and biochemistry of butylated hydroxy anisole and butylated hydroxy toluene. J Oil Chem Soc, 52, 59-62
  8. Matsuoka A, Furuta A, Ozaki M, Fukuhara K, Miyata N (2001) Resveratrol, a naturally occurring polyphenol, induces sister chromatid exchanges in a Chinese hamster lung (CHL) cell line. Mutat Res, 107, 494-495
  9. Rubin RR, Peyrot M (1999) Quality of life and diabetes. Diabetes Metab Res Rev, 15, 205-218<205::AID-DMRR29>3.0.CO;2-O
  10. Lee SH, Lee JK, Kim IH (2012) Trends and perspectives in the development of antidiabetic drugs for type 2 diabetes mellitus. Korean J Microbiol Biotechnol, 40, 180-185
  11. Lee EB, Na GH, Ryu CR, Cho MR (2004) The review on the study of diabetes mellitus in Oriental medicine journals. J Korean Orient Med, 25, 169-179
  12. Schwarz K, Mertz W (1959) Chromium (III) and the glucose tolerance factor. Arch Biochem Biophys, 85, 292-295
  13. Paul R, Jamie H, Phuong OT, Vincent P (2004) ${\beta}$-Cell glucose toxicity, lipotoxicity and chronic oxidative stress in type 2 diabetes. Diabetes, 53, 5119-5124
  14. Tsujimoto T, Shioyama E, Moriya K, Kawaratani H, Shirai Y, Toyohara M, Mitoro A, Yamao J, Fujii H, Fukui H (2008) Pneumatosis cystoides intestinalis following alpha-glucosidase inhibitor treatment : a case report and review of the literature. World J Gastroenterol, 14, 6087-6092
  15. Kihara Y, Ogami Y, Tabaru A, Unoki H, Otsuki M (1997) Safe and effective treatment of diabetes mellitus associated with chronic liver diseases with an alpha-glucosidase inhibitor, acarbose. J Gastroenterol, 32, 777-782
  16. Jung CM, Kwon HC, Choi SZ, Lee JH, Lee DJ, Ryu SN, Lee KR (2000) Phytochemical constituents of Ainsliaea acerifolia. Korean J Pharmacogn, 31, 125-129
  17. Bohlmann F, Chen ZL (1982) Guaianolides from Ainsliaea fragrans. Phytochem, 21, 2120-2122
  18. Adegawa S, Miyase T, Ueno A (1987) Sesquiterpene lactones from Diaspananthus uniflorus. Chem Pharm Bull, 35, 1479-1485
  19. Miyase T, Ozaki H, Ueno A (1991) Sesquiterpene glycosides from Aisliaea cordifolia Franch. et Sav. Chem Pharm Bull, 39, 937-938
  20. Jin H (1982) Studies on the constituents of Ainsliaea acerifolia Sch. -Bip. var. subapoda Nakai. Yakugaku Zasshi, 102, 911-922
  21. Blois MS (1958) Antioxidant activity determination by the use of a stable free radical. Nature, 181, 1199-1200
  22. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C (1999) Antioxidnt activity applying and improved ABTS radical cation decolorization assay. Free Radic Biol Med, 26, 1231-1237
  23. Eom SH, Lee SH, Yoon NY, Jung WK, Jeon YJ, Kim SK, Lee MS, Kim YM (2012) ${\alpha}$-glucosidase and ${\alpha}$ -amylase inhibitory activities of phlorotannins from Eisenia bicyclis. J Sci Food Agric, 92, 2084-2090
  24. Gao X, Bjor, L, Trajkovski V, Uggla M (2000) Evaluation of antioxidant activities of rosehip ethanol extracts in different test system. J Sci Food Agric, 80, 2021-2027<2021::AID-JSFA745>3.0.CO;2-2
  25. Lee S G, Yu MH, Lee S P, Lee IS (2008) Antioxidant activities and induction of apoptosis by methanol extracts from avocado. J Korean Soc Food Sci Nutr, 37, 269-275
  26. Wang SY, Chang HN, Lin KT, Lo CP, Yang NS, Shyur LF (2003) Antioxidant properties and phytochemical characteristics of extracts from Lactucaindica. J Agric Food Chem, 26, 1506-1512
  27. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med, 26, 1231-1237
  28. Shin JA, Lee JH, Kim HS, Choi JH, Yoon KH (2012) Prevention of diabetes : a strategic approach for individural patients. Diabets Metab Res Rev, 28, 79-84
  29. Bischoff H (1995) The mechanism of alpha-glucosidase inhibition in the management of diabetes. Clin Invest Med, 18, 303-311
  30. Nguyen MTT, Nguyen NTM, Nguyen HX, Huynh TNN, Min BS (2012) Screening of a-glucosidase inhibitory activity of Vietnamese medicinal plants : isolation of active principles from Oroxylum indicum. Nat Prod Sci, 18, 47-51

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