Effect of Chungkukjang supplementation on oxidative stress and antioxidant nutrients of diabetic rats induced by streptozotocin

청국장 식이 보충이 당뇨 쥐의 산화적 스트레스와 항산화 영양소 함량에 미치는 영향

  • Park, Heyun-Sook (Division of Hotel Culinary Arts, Daegu Technical College) ;
  • Kong, Hyun-Joo (Department of Herbal Food Science, Graduate School of Daegu Haany University) ;
  • Lee, Eon-Hee (Department of Food and Nutrition, Graduate School, Yeungnam University) ;
  • Choi, Eun-Mi (Department of Food Science and Nutrition, Graduate School, Kyungpook National University) ;
  • Jang, Joung-Hyeon (Division of Cosmetology, Dong-Eui Institute of Technology) ;
  • Lee, Myoung-Hee (Department of beauty care, Songho college) ;
  • Hong, Ju-Yeon (Faculty of Herbal Food Cuisine and Nutrition, Daegu Haany University) ;
  • Hwang, Su-Jung (Faculty of Herbal Food Cuisine and Nutrition, Daegu Haany University) ;
  • Jung, Hyeon-A (Faculty of Herbal Food Cuisine and Nutrition, Daegu Haany University) ;
  • Yang, Kyung-Mi (Faculty of Herbal Food Cuisine and Nutrition, Daegu Haany University)
  • 박현숙 (대구공업대학교 식음료계열) ;
  • 공현주 (대구한의대학교 대학원 한방식품학과) ;
  • 이언희 (영남대학교 대학원 식품영양학과) ;
  • 최은미 (경북대학교 대학원 식품영양학과) ;
  • 장정현 (동의과학대학교 미용계열) ;
  • 이명희 (송호대학교 뷰티케어과) ;
  • 홍주연 (대구한의대학교 한방식품조리영양학부) ;
  • 황수정 (대구한의대학교 한방식품조리영양학부) ;
  • 정현아 (대구한의대학교 한방식품조리영양학부) ;
  • 양경미 (대구한의대학교 한방식품조리영양학부)
  • Received : 2014.09.16
  • Accepted : 2015.02.12
  • Published : 2015.04.30


This study was conducted to examine the effects of a supplemented Chungkukjang diet on oxidative stress and antioxidant nutrients in Streptozotocin (STZ, 45 mg/kg of BW, IP injection)-induced diabetic rats. Diets that contained soybean Chungkukjang powder (SC), Yakkong Chungkukjang powder (YC), and Yakkong Chungkukjang powder with black food added (YCB) were administered to the STZ-induced diabetic rats for seven weeks. The increased lipid peroxide contents of their serum and liver were slightly controlled by providing them three types of Chungkukjang. The retinol level in the serum was 7.5 times higher in the STZ-induced diabetic group after the provision of YC. The total antioxidant capacity (TAC) level in the serum was higher in the STZ-induced diabetic group after the provision of YCB. Also, the retinol and tocopherol levels in the liver of the STZ-induced diabetic rats increased after they were provided YCB, and the decreased reduced glutathione (GSH) /oxidized glutathione (GSSG) level in their liver improved after they were fed a diet that contained YC. Moreover, the decreased anthocyanin level in the liver of the STZ-induced diabetic group improved after the provision of three types of Chungkukjang powder. These findings suggest that the Chungkukjang diet is a valuable food for the management of the health of diabetic patients and for the prevention of diabetic complications.


  1. Burk RF, Trumble MJ, Lawrance RA (1980) Rat hepatic cytosolic glutathione-dependent enzyme protection against lipid peroxidation in the NADPH-microsomal lipid peroxidation-system. Biochem Biophys Acta, 618, 35-41
  2. Opara EC, Abdel-Rahman E, Ssoliman S, Kamel WA, Souka S, Lowe JE, Abdel-Aaleem S (1999) Depletion of total antioxidant capacity in type 2 diabetes. Metabolism, 48, 1414-1417
  3. Kim JH, Kim MJ (2004) Dietary intakes and plasma antioxdant vitamins levels in Korean elderly with diabetes. Asia Pac J CLin Nutr, 13, S152
  4. Kim YH, Yun HT, Park KY (2004) Biological effects of black colored soybean. Plant Resources, 7, 195-199
  5. Ryu SH (2001) Studies on antioxidative effects and antioxidative componets of soybean and Chongkukjang. Ph D Thesis, Inje University, Korea, p 46-47
  6. Bieri JG, Tolliver JJ, Catignani GL (1979) Simultaneous determination of ${\alpha}$-tocopherol and retinol in plasma or red cell by high pressure liquid chromatography. J Clin Nutr, 32, 2143-2148
  7. Furr HC, Amedee-Manesme O, Olson JA (1984) Gradient reversed-phased high-performance liquid chromatographic separation of naturally occurring retinoids. J Chromato, 309, 299-307
  8. Theodrous PM, Helmut S (1981) Assay of glutathione, glutathione disulfide, and glutathione mixed disulfides in biological samples. Method Enzymology, 77, 373-382
  9. Park YE, Cho JH, Cho HM, Yi JY, Seo HW, Choung MG (2009) A new potato cultivar "Hongyoung", with red skin and flesh color, and high concentrations of anthocyanins. Korean J Breed Sci, 41, 502-506
  10. Fuleki T, Francis FJ (1968) Quantitative methods for anthocyanins. 2. Determination of total anthocyanin and degradation index for cranberry juice. J Food Sci, 33, 78-82
  11. Freitas JP, Filipe PM, Rodrigo FG (1997) Lipid peroxidation in type 2 normolipidemic diabetic patients. Diabetes Res Clin Prac, 36, 71-76
  12. Havel PJ, Uriu-Hare JY, Liu T, Stanhope KL, Stern JS, Keen CL, Ahren BO (1998) Marked and rapid decrease of circulating leptin in streptozotocin diabetic rats : reversal by insulin. Am J Physiol, 274, R1482-R1491
  13. Yan SD, Schmidt AM, Anderson CM, Zhang J, Brett J, Zou YS, Pinsky D, Stern D (1994) Enhanced cellular oxidant stress by the interaction of advanced glycation end products with their receptor/binding protein. J Biol Chem, 269, 9889-9897
  14. Arai K, Maguchi S, Fujii S, Ishibashi H, Oikawa K, Taniguchi N (1987) Glycation and inactivation of human Cu-Zn-superoxide dismutase. J Biol Chem, 262, 16969-16972
  15. KIm SS, Son SM (2008) Oxidative stress and cell dysfunction in diabetes : role of ROS produced by mitochondria and NAD(P)H oxidase. Korean Diabetes J, 32, 389-398
  16. Sundaram RK, Bhaskar A, Vijayalingam S, Viswanthan M, Mohan R, Shanmugasundaram KR (1996) Antioxidant status and lipid peroxidation in type II diabetes mellitus with and without complication. Clin Sci, 90, 255-260
  17. Dave GS, Kalia K (2001) Hyperglycemia induced oxidative stress in type-1 and type-2 diabetic patients with and without nephropathy. Cell Mol Biol, 53, 68-78
  18. Ceriello A, Bortolotti N, Falleti E, Taboga C, Tonutti L, Crescentini A, Motz E, Lizzio S, Russo A, Bartoli E (1997) Total radical trapping antioxidant parameter in NIDDM patients. Diabetes Care, 20, 194-197
  19. Seo JS, Yang KM, Choi MJ (1995) Effects of dietary vitamin A on the status of antioxidants in ethanol-treatedrats. J Korean Soc Food Nutr, 24, 848-858
  20. Videla LA (1984) Chemically induced antioxidantsenstive respiration. Relation to glutathione content and lipid peroxidation in the perfused rat liver. Fed Eur Biochem Soc, 178, 119-121
  21. Atkinson MA, Maclaren NK (1994) The pathogenesis of insulin-dependent diabetes mellitus. N Engl J Med, 331, 1428-1436
  22. Behr GA, Silva EG, Ferreira AR, Cerski CT, Dal-Pizzol F, Moreira JC (2008) Pancreas beta-cells morphology, liver antioxidant enzymes and liver oxidative parameters in alloxan-resistant and alloxan-susceptible Wistar rats : a viable model system for the study of concepts into reactive oxygen species. Fundam Clin Pharmacol, 22, 657-666
  23. Santini SA, Marra G, Giardina B, Cotroneo P, Mordente, A, Martorana GE, Manto A, Ghirlanda G (1997) Defective plasma antioxidant defense and enhanced susceptability to lipid peroxidant in uncomplicated IDDM. Diabetes, 46, 1853-1858
  24. Akitha MK, Gondi M, Sakthivelu G, Giridhar P, Rajasekaran T, Ravishankar GA (2009) Functional attributes of soybean seeds and products, with reference to isoflavone content and antioxidant activity. Food Chem, 114, 771-776
  25. Joo EY, Park CS (2010) Antioxidative and fibrinolytic activity of extracts from soybean and Chungkukjang(fermented soybeans) prepared from a black soybean cultivar. Korean J Food Preserv, 17, 874-880
  26. Ramirez-Tortosa C, Andersen OM, Gardner PT, Morrice PC, Wood SG, Duthie SJ, Collins AR, Duthie GG (2001) Anthocyanin-rich extract decreases indices of lipid peroxidation and DNA damage in vitamin E depleted rats. Free Radic Biol Med, 31, 1033-1037
  27. Jung MG, Lim JD (2012) Antioxidant, anticancer and immune activation of anthocyanin fraction from Rubus coreanus Miquel fruits (Bokbunja). Korean J Medicinal Crop Sci, 20, 259-269
  28. Park HS, Jang YJ, Choi DS, Namgung MA, Lee YJ, Jang SA (1995) Increased oxidative stress in Sciatic nerves of streptozotocin induced diabetic rats : lack of vitamin C effect. Diabetes, 19, 279-286
  29. Park SK, Park NY, Lim YS (2009) Effects of short term antioxidant cocktail supplementation on the oxidative stress and inflammatory response of renal inflammation in diabetic mice. Korean J Nutr, 42, 673-681
  30. Myung JE, Hwang IK (2008) Functional components and antioxidative activities of soybean extracts. Korean Soybean Digest, 25, 23-29
  31. Wei H, Cai Q, Rahn R (1996) Inhibition of UV light and fenton reaction-induced oxidative DNA damage by the soybean isoflavone genistein. Carcinogenesis, 17, 73-78
  32. Lee MH, Park YH, Oh HS, Kwak TS (2002) Isoflavone content in soybean and its processed products. Food Sci Biotechnol, 34, 365-369
  33. Philip GR, Forrest HN, George CF (1999) AIN-93 purified diets for laboratory rodents : final report of the American institute of nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J Nutr, 123, 1939-1951
  34. Ohkwwa H, Ohishi N, Yagi K (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem, 95, 351-358
  35. Aebi H (1974) Catalase. In : Methods of enzymatic analysis Begmeyer HU editor. Verlag Chemie Academic Press. New York USA, p 673-684

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