DOI QR코드

DOI QR Code

Alignment change of lipid molecules in lipid bilayers by an antimicrobial peptide protegrin-1

지질 이중막에 결합된 항균성 펩타이드 protegrin-1에 의한 지질 분자의 정열도 변화

  • Received : 2015.04.04
  • Accepted : 2015.04.12
  • Published : 2015.04.25

Abstract

Changes in antimicrobial peptide-lipid mixtures were investigated using 31P solid-state nuclear magnetic resonance spectroscopy. An antimicrobial peptide, protegrin-1, and phosphatidylcholine were deposited on a thin cover glass and incubated under a relative humidity of 95%. The changes in the mixtures were observed after hydration or air-drying. How repetitive hydration and drying changed the phase of the sample was also observed. The degrees of disruption of the well-aligned bilayers of phosphatidylcholine were determined quantitatively by simulating the experimental spectra. The peptide-lipid mixtures changed reversibly after hydration and drying, and the samples reached an equilibrium state after several repetitions.

Keywords

antimicrobial peptide;protegrin-1;hydration;phase change

References

  1. Y. Bai, S. Liu, P. Jiang, L. Zhou, J. Li, C. Tang, C. Verma, Y. Mu, R. W. Beuerman and K. Pervushin, Biochemistry, 48, 7229-7239 (2009). https://doi.org/10.1021/bi900670d
  2. R. Latorre and O. Alvarez, Physiol Rev, 61, 77-150 (1981).
  3. H. Steiner, D. Hultmark, A. Engstrom, H. Bennich and H. G. Boman, Nature, 292, 246-248 (1981). https://doi.org/10.1038/292246a0
  4. J. Y. Lee, A. Boman, C. X. Sun, M. Andersson, H. Jornvall, V. Mutt and H. G. Boman, Proc. Natl. Acad. Sci. U.S.A, 86, 9159-9162 (1989). https://doi.org/10.1073/pnas.86.23.9159
  5. M. R. Yeaman and N. Y. Yount, Pharmacol. Rev., 55, 27-55 (2003). https://doi.org/10.1124/pr.55.1.2
  6. R. Mani, A. J. Waring, R. I. Lehrer and M. Hong, Biochim. et Biophs. Acta,-biomembr, 1716, 11-18 (2005). https://doi.org/10.1016/j.bbamem.2005.08.008
  7. Toke, O. Biopolymers, 80, 717-735 (2005). https://doi.org/10.1002/bip.20286
  8. S. J. Ludtke, K. He, W. T. Heller, T. A. Harroun, L. Yang and H. W. Huang, Biochemistry, 35, 13723-13728 (1996). https://doi.org/10.1021/bi9620621
  9. K. Matsuzaki, O. Murase, N. Fujii and K. Miyajima, Biochemistry, 35, 11361-11368 (1996). https://doi.org/10.1021/bi960016v
  10. J. Z. Gesell, M. and S. J. Opella, J. Biomol. NMR, 9, 127-135 (1997). https://doi.org/10.1023/A:1018698002314
  11. K. Matsuzaki, O. Murase and K. Miyajima, Biochemistry, 34, 12553-12559 (1995). https://doi.org/10.1021/bi00039a009
  12. I. Marcotte, L. Wegener Kate, Y.-H. Lam, C. S. Chia Brian, R. R. de Planque Maurits, H. Bowie John, M. Auger and F. Separovic, Chem. and Phys. of Lipids, 122, 107-116 (2003). https://doi.org/10.1016/S0009-3084(02)00182-2
  13. L. Yang, T. M. Weiss, R. I. Lehrer and H. W. Huang, Biophys. J., 79, 2002-2007 (2000). https://doi.org/10.1016/S0006-3495(00)76448-4
  14. C. Munster, A. Spaar, B. Bechinger and T. Salditt, Biochim. et Biophs. Acta,-biomembr., 1562, 37-44 (2002). https://doi.org/10.1016/S0005-2736(02)00357-7
  15. R. W. S. Glaser, Durr, C., Wadhwani, U. H. N., Afonin, P., Strandberg, S. E. and A. S. Ulrich, Biophys. J., 88, 3392-3397 (2005). https://doi.org/10.1529/biophysj.104.056424
  16. K. J. Hallock, D. K. Lee and A. Ramamoorthy, Biophys. J., 84, 3052-3060 (2003). https://doi.org/10.1016/S0006-3495(03)70031-9
  17. J. J. Buffy, T. Hong, S. Yamaguchi, A. J. Waring, R. I. Lehrer and M. Hong, Biophys. J., 85, 2363-2373 (2003). https://doi.org/10.1016/S0006-3495(03)74660-8
  18. V. N. Kokryakov, S. S. Harwig, E. A. Panyutich, A. A. Shevchenko, G. M. Aleshina, O. V. Shamova, H. A. Korneva and R. I. Lehrer, FEBS Lett, 327, 231-236 (1993). https://doi.org/10.1016/0014-5793(93)80175-T
  19. R. L. Fahrner, T. Dieckmann, S. S. L. Harwig, R. I. Lehrer, D. Eisenberg and J. Feigon, Chem. Biol., 3, 543-550 (1996). https://doi.org/10.1016/S1074-5521(96)90145-3
  20. S. Yamaguchi, T. Hong, A. Waring, R. I. Lehrer and M. Hong, Biochemistry, 41, 9852-9862 (2002). https://doi.org/10.1021/bi0257991
  21. R. Mani, J. J. Buffy, A. J. Waring, R. I. Lehrer and M. Hong, Biochemistry, 43, 13839-13848 (2004). https://doi.org/10.1021/bi048650t
  22. C. Kim, J. Spano, E. K. Park and S. Wi, Biochim Biophys Acta, 1788, 1482-1496 (2009). https://doi.org/10.1016/j.bbamem.2009.04.017
  23. S. Wi and C. Kim, J. Phys. Chem. B, 112, 11402-11414 (2008). https://doi.org/10.1021/jp801825k