Alignment change of lipid molecules in lipid bilayers by an antimicrobial peptide protegrin-1

지질 이중막에 결합된 항균성 펩타이드 protegrin-1에 의한 지질 분자의 정열도 변화

  • Received : 2015.04.04
  • Accepted : 2015.04.12
  • Published : 2015.04.25


Changes in antimicrobial peptide-lipid mixtures were investigated using 31P solid-state nuclear magnetic resonance spectroscopy. An antimicrobial peptide, protegrin-1, and phosphatidylcholine were deposited on a thin cover glass and incubated under a relative humidity of 95%. The changes in the mixtures were observed after hydration or air-drying. How repetitive hydration and drying changed the phase of the sample was also observed. The degrees of disruption of the well-aligned bilayers of phosphatidylcholine were determined quantitatively by simulating the experimental spectra. The peptide-lipid mixtures changed reversibly after hydration and drying, and the samples reached an equilibrium state after several repetitions.


antimicrobial peptide;protegrin-1;hydration;phase change


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