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Establishment and application of a qualitative real-time polymerase chain reaction method for detecting genetically modified papaya line 55-1 in papaya products

RT-PCR을 이용한 유전자변형파파야(55-1)검사법 확립 및 파파야가공식품의 적용 연구

  • Kwon, Yu Jihn (Hazardous Substances Analysis Team, Center for Food and Drug Analysis, Gyeongin Regional Food and Drug Administration) ;
  • Chung, So Young (Hazardous Substances Analysis Team, Center for Food and Drug Analysis, Gyeongin Regional Food and Drug Administration) ;
  • Cho, Kyung Chul (Hazardous Substances Analysis Team, Center for Food and Drug Analysis, Gyeongin Regional Food and Drug Administration) ;
  • Park, ji Eun (Hazardous Substances Analysis Team, Center for Food and Drug Analysis, Gyeongin Regional Food and Drug Administration) ;
  • Koo, Eun Joo (Hazardous Substances Analysis Team, Center for Food and Drug Analysis, Gyeongin Regional Food and Drug Administration) ;
  • Seo, Dong Hyuk (Hazardous Substances Analysis Team, Center for Food and Drug Analysis, Gyeongin Regional Food and Drug Administration) ;
  • Kim, Eugene (Hazardous Substances Analysis Team, Center for Food and Drug Analysis, Gyeongin Regional Food and Drug Administration) ;
  • Whang, Jehyun (Hazardous Substances Analysis Team, Center for Food and Drug Analysis, Gyeongin Regional Food and Drug Administration) ;
  • Park, Seong Soo (Hazardous Substances Analysis Team, Center for Food and Drug Analysis, Gyeongin Regional Food and Drug Administration) ;
  • Choi, Sun Ok (Hazardous Substances Analysis Team, Center for Food and Drug Analysis, Gyeongin Regional Food and Drug Administration) ;
  • Lim, Chul Joo (Hazardous Substances Analysis Team, Center for Food and Drug Analysis, Gyeongin Regional Food and Drug Administration)
  • 권유진 (경인지방식품의약품안전청 시험분석센터 유해물질분석팀) ;
  • 정소영 (경인지방식품의약품안전청 시험분석센터 유해물질분석팀) ;
  • 조경철 (경인지방식품의약품안전청 시험분석센터 유해물질분석팀) ;
  • 박지은 (경인지방식품의약품안전청 시험분석센터 유해물질분석팀) ;
  • 구은주 (경인지방식품의약품안전청 시험분석센터 유해물질분석팀) ;
  • 서동혁 (경인지방식품의약품안전청 시험분석센터 유해물질분석팀) ;
  • 김유진 (경인지방식품의약품안전청 시험분석센터 유해물질분석팀) ;
  • 황지현 (경인지방식품의약품안전청 시험분석센터 유해물질분석팀) ;
  • 박성수 (경인지방식품의약품안전청 시험분석센터 유해물질분석팀) ;
  • 최선옥 (경인지방식품의약품안전청 시험분석센터 유해물질분석팀) ;
  • 임철주 (경인지방식품의약품안전청 시험분석센터 유해물질분석팀)
  • Received : 2014.12.08
  • Accepted : 2015.04.13
  • Published : 2015.04.25

Abstract

Genetically modified (GM) papaya line 55-1, which is resistant to PRSV infection, has been marketed globally. Prompt and sensitive protocols for specific detections are essential for the traceability of this line. Here, an event- and construct-specific real-time polymerase chain reaction (RT-PCR) method was established to detect 55-1. Qualitative detection was possible for fresh papaya fruit up to dilutions of 0.005% and 0.01% for the homozygous SunUp and heterozygous Rainbow cultivars, respectively, in non-GM papaya. The method was applied in the qualitative detection of 55-1 in eight types of commercially processed papaya products. Additionally, papaya products were monitored to distinguish GM papaya using the P35S and T-nos RT-PCR detection methods. As expected, detection capacity was improved via modified sample preparation and the established RT-PCR detection method. Taking these results together, it can be suggested that a suitable method for the extraction and purification of DNA from processed papaya products was established for the detection of GM papaya.

Keywords

genetically modified;real-time polymerase chain reaction;papaya;55-1;monitoring

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