DOI QR코드

DOI QR Code

Determination of terbutaline in human plasma by coupled column chromatography

커플드칼럼크로마토그래피에 의한 사람 혈장 중 테르부탈린의 정량

  • Received : 2014.08.18
  • Accepted : 2015.03.23
  • Published : 2015.04.25

Abstract

A method was developed and fully validated for the determination of terbutaline, a β2-receptor agonist, in human plasma. Plasma samples were prepared by solid-phase extraction with Sep-Pak silica, followed by high-performance liquid chromatography (HPLC). The terbutaline was pre-separated from the interfering components in plasma on a Luna C18 (2) column, and terbutaline and salbutamol as an internal standard were resolved and determined on a Luna Silica column. The two columns were connected by a switching valve equipped with silica pre-column. The pre-column was used to concentrate the terbutaline in the eluent from the C18 column before back-flushing onto the silica column with fluorescence detection at an excitation/emission wavelength of 276/306 nm. The method was shown to be specific by testing six different human plasma sources. Linearity was established for a concentration range of 0.4-20.0 ng/mL with a correlation coefficient of 0.9999. The lower limit of quantitation was 0.4 ng/mL with a precision of 10.1% as C.V.%.

Keywords

terbutaline;coupled column chromatography;HPLC;solid phase extraction;plasma

References

  1. Y. Arai, T. Fukushima, M. Shirao, X. Yang and K. Imai, Biomed. Chromatogr., 14, 118-124 (2000). https://doi.org/10.1002/(SICI)1099-0801(200004)14:2<118::AID-BMC936>3.0.CO;2-O
  2. R. Wyss, F. Bucheli and W. Philipp, J. Chromatogr. A., 870, 189-198 (2000). https://doi.org/10.1016/S0021-9673(99)01016-X
  3. K. H. Kim, H. J. Kim, J. H. Kim and S. D. Shin, J, Chromatpgr. B., 751, 69-77 (2001). https://doi.org/10.1016/S0378-4347(00)00449-7
  4. Y. Zhang and Z. R. Zhang, J. Chromatpgr. B., 805, 211-214 (2004). https://doi.org/10.1016/j.jchromb.2004.02.037
  5. M. T. Acekrmans, J. L. Beckers, F. M. Everaerts and I. G. J. A. Seelen, J. Chromatogr., 590, 341-353 (1992). https://doi.org/10.1016/0021-9673(92)85397-C
  6. G. A. Jacobson and G. M. Peterson, J. Pharm. Biomed. Anal., 12, 825-832 (1994). https://doi.org/10.1016/0731-7085(94)E0006-M
  7. United States Pharmacopeia (USP30) and National formulary (NF 25), United State Pharmacopeial Convention, Rockville, MD (2007).
  8. British Pharmacopoeia (BP 2013), British Pharmacopoeia Commission, Market Towers, London (2013).
  9. R. A. Clare, D. S. Davies and T. A. Baillie, Biomed. Mass. Spectrom., 6, 31-37 (1979). https://doi.org/10.1002/bms.1200060108
  10. S. E. Jacobsson, S. Jonsson, C. Lindberg and L. A. Svensson, Biomed. Mass. Spectrom., 7, 265-268 (1980). https://doi.org/10.1002/bms.1200070608
  11. M. Caban, P. Stepnowski, M. Kwiatkowski, N. Migowska and J. Kumirska, J. Chromatogr. A., 1281, 8110-8122 (2011).
  12. V. L. Herring and J. A. Johnson, J. Chromatogr. B., 741, 307-312 (2000). https://doi.org/10.1016/S0378-4347(00)00107-9
  13. Y. Q. Xia, D. Q. Liu and R. Bakhtiar, Chirality, 14, 742-749 (2002). https://doi.org/10.1002/chir.10135
  14. T. Zhou, T. Zhao, Q. Cheng, S. Liu, L. Xu and W. Tan, Biomed. Chromatogr., 28, 994-1002 (2014). https://doi.org/10.1002/bmc.3107
  15. P. T. McCarthy, S. Atwal, A. P. Sykes and J. G. Ayres, Biomed. Chromatogr., 7, 25-28 (1993). https://doi.org/10.1002/bmc.1130070107
  16. K. H. Kim, H. J. Kim, S. P. Hong and S. D. Shin, Arch. Pharm. Res., 23(5), 441-445, (2000). https://doi.org/10.1007/BF02976568