DOI QR코드

DOI QR Code

고지방 식이로 유도된 비만 마우스에서 대두 부산물인 순물과 침지수의 항비만 효과

박영미;임재환;서을원
Park, Young Mi;Lim, Jae Hwan;Seo, Eul Won

  • 투고 : 2014.10.01
  • 심사 : 2014.12.08
  • 발행 : 2015.04.30

초록

본 연구는 대두 부산물인 순물과 침지수가 고지방 식이에 의해 비만이 유도된 마우스의 지방 제거에 미치는 영향을 조사하였다. 침지수가 포함된 일반 사료를 섭이한 실험군의 체중 및 간과 부고환 지방 조직 내 지방 축적은 현저히 적은 것으로 나타났으며, 복부 내 내장 지방과 피하지방도 크게 발달하지 않은 것으로 나타나 침지수는 체내 축적된 지방량을 효과적으로 감소시키는 것으로 조사되었다. 침지수 식이군의 혈중 AST와 ALT의 활성은 대조군 수준으로 유지되었으며, 콜레스테롤 함량과 중성 지질 농도가 감소되는 것으로 나타나 침지수가 고지방 식이로 인한 혈중 효소 활성과 지질농도를 개선하는 것으로 조사되었다. 특히 침지수는 metabolic sensor 단백질인 AMPK와 ACC의 인산화를 촉진시켜 체내 지방산 산화에 영향을 주는 것으로 확인되었다. 또한 침지수는 복부 내 피하지방과 내장지방의 축적을 억제하는 것으로 확인되었다. 따라서 대두 부산물 중 침지수는 비만이 유도된 마우스의 혈중 지질 함량을 개선할 뿐만 아니라 체내 조직의 지방 축적을 완화시키거나 제거하는 데 효과적일 것으로 사료된다.

키워드

Soybean curd whey;Soybean soaking water;Micro-CT;Lipid metabolism;Anti-obesity

참고문헌

  1. Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254. https://doi.org/10.1016/0003-2697(76)90527-3
  2. Adlercreutz, H., Y. Mousavi, J. Clark, K. Hockerstedt, E. Hamalainen, K. Wahala, T. Makela and T. Hase. 1992. Dietary phytoestrogens and cancer: in vitro and in vivo studies. J. Steroid Biochem. Mol. Biol. 41:331-337. https://doi.org/10.1016/0960-0760(92)90359-Q
  3. Ahn, I.S., K.Y. Pack and M.S. Do. 2007. Weight control mechanisms and antiobesity functional agents. J. Korean Soc. Food Sci. Nutr. 36:503-513 (in Korean). https://doi.org/10.3746/jkfn.2007.36.4.503
  4. Anne, W.H. and B.H. Joyce. 2001. Differential effects of flavonoids on 3T3-L1 adipogenesis and lipolysis. Am. J. Physiol. Cell Physiol. 280:C807-C813.
  5. Bae, E.A., T.W. Kwon and G.S. Moon. 1997. Isoflavone contents and antioxidative effects of soybean, soybean curd and their by-products. J. Korean Soc. Food Sci. Nutr. 26:371-375 (in Korean).
  6. Biorntorp, P. 1988. The associations between obesity, adipose tissue distribution and disease. Acta. Med. Scand. 723:121-134.
  7. Chen, J.R., S.F. Chiou, K. Suetsuna, H.Y. Yang and S.C. Yang. 2003. Lipid metabolism in hypercholesterolemic rats affected by feeding cholesterol-free diets containing different amounts of non dialyzed soybean protein fraction. Nutrition 19:676-680. https://doi.org/10.1016/S0899-9007(03)00072-8
  8. Chin, H.S., K.J. Pack, S.H. Pack and J.K. Kim. 2009. The effects of herbal extract mixture on anti-obesity. J. Korean Soc. Food Sci. Nutr. 38:32-38 (in Korean). https://doi.org/10.3746/jkfn.2009.38.1.032
  9. Choi, Y.B., K.S. Kim and H.S. Sohn. 1995. Recovery of soy-oligosaccharides using calcium oxide. J. Food Sci. 27:225-229 (in Korean).
  10. Despres, J.P. 1993. Abdominal obesity as important component of insulin-resistant syndrome. Nutrition 19:452-459.
  11. Friedewald, W.T., R.I. Levy and D.S. Fredrickson. 1972. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin. Chem. 18:499-502.
  12. Frings, C.S. and R.M. Dunn. 1970. A colorimetric method for determination of total serum lipids based on the sulfo-phospho-vanillin reaction. Am. J. Clin. Pathol. 53:89-91. https://doi.org/10.1093/ajcp/53.1.89
  13. Kim, A.R., J.J. Lee, S.S. Cha, H.C. Chang and M.Y. Lee. 2012. Effect of soybeans, chungkukjang, and doenjang on blood glucose and serum lipid profile in streptozotocin-induced diabetic rats. J. Korean Soc. Food Sci. Nutr. 41:621-629 (in Korean). https://doi.org/10.3746/jkfn.2012.41.5.621
  14. Galvez, A.F. and B.O. de Lumen. 1999. A soybean cDNA encoding a chromatin-binding peptide inhibits mitosis of mammalian cells. Nat. Biotechnol. 17:495-500. https://doi.org/10.1038/8676
  15. Hackler, L.R. and B.R. Stillings. 1967. Amino acid compostion of heat-processed soymilk and its correlation with nutritive value. Cereal Chem. 44:70-77.
  16. Kaufman, P.B., J.A. Duke, H. Brielmann, J. Boik and J.E. Hoyt. 1997. A comparative survey of leguminous plants as sources of the isoflavones, genistein and daidzein: implications for human nutrition and health. J. Altern. Complement Med. 3:7-12. https://doi.org/10.1089/acm.1997.3.7
  17. Kim, K.S., H.K. Chun and H.S. Sohn. 1994. Purification of oligosaccharides from soybean using activated charcoal. Korean J. Food Sci. Technol. 3:156-156 (in Korean).
  18. Kim, K.S., J.H. Hong and D.I. Kim. 2012. Anti-obesity effects of capsule-filled cheonggukjang added with arrowroot (Pueraria thunbergiana) extracts in ob/ob mice. J. Korean Soc. Food Sci. Nurt. 41:782-789 (in Korean). https://doi.org/10.3746/jkfn.2012.41.6.782
  19. Koo, H.J., S.C. Kang, S.A. Jang, J.E. Kwon, E.S. Sohn and E.H. Sohn. 2014. Effects of protocatechuic acid derived from Rubus coreanus on the lipid metabolism in high cholesterol diet induced mice. Korean J. Plant Res. 27:271-278 (in Korean). https://doi.org/10.7732/kjpr.2014.27.4.271
  20. Kopelman, P.G. and L. Albon. 1997. Obesity, non-insulin-dependent diabetes mellitus and the metabolic syndrome. Brit. Med. Bull. 53:322-340. https://doi.org/10.1093/oxfordjournals.bmb.a011616
  21. Lee, H.S., J.H. Choi, Y.E. Kim and C.H. Lee. 2012. Effect of dietary intake of Salicornia herbacea L. hot water extract on anti-obesity in diet-induced obese rats. J. Korean Soc. Food Sci. Nutr. 41:950-956 (in Korean). https://doi.org/10.3746/jkfn.2012.41.7.950
  22. Noma, A., K. Nezu-Nakayama, M. Kita and H. Okabe. 1978. Simultaneous determination of serum cholesterol in high- and low-density lipoproteins with use of heparin, Ca2+, and an anion-exchange resin. Clin. Chem. 24:1504-1508.
  23. Lew, E.A. 1985. Mortality and weight: insured lives and the american cancer society studies. Ann. Intern. Med. 103:1024-1029. https://doi.org/10.7326/0003-4819-103-6-1024
  24. Matsumoto, K., Y. Watanabe and S. Yokoyama. 2007. Okara, soybean residue, prevents obesity in a diet-induced murine obesity model. Biosci. Biotechnol. Biochem. 71:720-727. https://doi.org/10.1271/bbb.60563
  25. McGee, D.L. 2005. Body mass index and mortality: a meta-analysis based on person-level data from twenty-six observational studies. Ann. Epidemiol. 15:87-97. https://doi.org/10.1016/j.annepidem.2004.05.012
  26. Potter, S.M. 1995.Overview of proposed mechanisms for the hypocholesterolemic effect of soy. J. Nutr. 125:606S-611S.
  27. Reitman, S. and S.A. Frankel. 1957. Colorimetric method for determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. Am. J. Clin. Pathol. 28:56-63. https://doi.org/10.1093/ajcp/28.1.56
  28. Richmond, W. 1976. Use of cholesterol oxidase for assay of total and free cholesterol in serum by continuous flow analysis. Clin. Chem. 22:1579-1588.
  29. Ruderman, N.B, A.K. Saha, D. Vavvas and L.A. Witters. 1999. Malonyl-CoA, fuel sensing, and insulin resistance. Am.J. Physiol. 276:1-18.
  30. Saha, A.K.R.N. 2003. Malonyl-CoA and AMP-activated protein kinase: An expanding partnership. Mol. Cell Biochem. 253:65-70. https://doi.org/10.1023/A:1026053302036
  31. Sharma, O.P., H. Adlercreutz, J.D. Strandberg, B.R. Zirkin, D.S. Coffey and L.L. Exing. 1992. Soy of dietary source playsd a preventive role against the pathogenesis of prostatitis in rats. J. Steroid. Biochem. Mol. Biol. 43:557-564. https://doi.org/10.1016/0960-0760(92)90244-D
  32. Wei, H.L., F. Frenkel, R. Browen and S. Barnes. 1993. Inhibition of tumor promoter-induced hydrogen peroxide formation in vitro and in vivo by genistein. Nutr. Cancer 20:1-20. https://doi.org/10.1080/01635589309514265
  33. Sollof, L.A., H.L. Futenberg and A.G. Lacko. 1973. Serum cholesterol esterfication in patients with coronary artery disease. Am. Heart J. 85:153-161. https://doi.org/10.1016/0002-8703(73)90455-9
  34. Villanueva, M.J., W.H. Yokoyama, Y.J. Hong, G.E. Barttley and P. Rupérez. 2011. Effect of high-fat diets supplemented with okara soybean by-product on lipid profiles of plasma, liver and faeces in syrian hamsters. Food Chem. 124:72-79. https://doi.org/10.1016/j.foodchem.2010.05.106
  35. Wat, E., S. Tandy, E. Kapera, A. Kamili, R.W.S. Chung, A. Brown, A. Brown, M. Rowney and J.S. Cohn. 2009. Dietary phospholipid-rich dairy milk extract reduces hepatomegaly, hepatic steatosis and hyperlipidemia in mice fed a high fat diet. Atherosclerosis 205:144-150. https://doi.org/10.1016/j.atherosclerosis.2008.12.004
  36. Won, C.U., Y.S. Jung, K.H. Yoon, H.Y. Lee, M.C. Yoon, B.K. Kim, S.D. Park and S.S. Shin. 2008. Herba ephedrae and Rhizoma amorphophalli modulates visceral obesity in micro-CT of high fat induced obese male mice. J. Korean Ori. Med. Pres. 16:205-217 (in Korean).

피인용 문헌

  1. The Effect of Bangpungtongsungsan on Allergic Rhinitis Of Obese Mouse Fed High Fat Diet. vol.29, pp.1, 2016, https://doi.org/10.7732/kjpr.2015.28.2.168
  2. Ethanol Extract of Hippophae Rhamnoides L. Leaves Inhibits Adipogenesis through AMP-activated protein kinase (AMPK) Activation in 3T3-L1 Preadipocytes vol.28, pp.5, 2015, https://doi.org/10.7732/kjpr.2015.28.2.168
  3. Anti-obesity Effects of Peucedanum japonicum Thunberg L. on 3T3-L1 Cells and High-fat Diet-induced Obese Mice vol.29, pp.1, 2016, https://doi.org/10.7732/kjpr.2015.28.2.168