Analysis of responses to physical stimuli in Caenorhabditis elegans using a microfluidic system

미세유체시스템을 이용한 예쁜꼬마선충의 물리적 자극에 대한 반응 분석

  • Published : 2015.08.31


Caenorhabditis elegans (C.elegans) has various interesting behavioral properties, such as chemotaxis, thermotaxis, and electrotaxis. However, most previous research were only focused on single-stimulus for studying its behavioral properties. In this paper, we propose a simple and effective device for analyzing the behavior of C.elegans with combined stimuli, electric field and temperature. We compared and analyzed wild type worms (N2) and four mutant worms (tax-4, ttx-7, unc-54, unc-6). We analyzed the reaction of worms to certain stimulus and identified that this device is effective to apply a combined stimulus.



Supported by : 한국연구재단


  1. S. Brenner, "The Genetics of Caenorhabditis elegans". Genetics, 77 (1): 71-94, 1974.
  2. Titus Kaletta1, Michael O. Hengartner, "Finding function in novel targets: C. elegans as a model organism", Nature Reviews Drug Discovery 5, 387-399, 2006.
  3. Pouya Rezai, Asad Siddiqui, Ponnambalam Ravi Selvaganapathy and Bhagwati P. Gupta, "Electrotaxis of Caenorhabditis elegans in a microfluidic environment", Lab Chip,10, pp. 220-226, 2010.
  4. Tsubasa Kimata, Hiroyuki Sasakura, Noriyuki Ohnishi, Nana Nishio1 and Ikue Mori., "Thermotaxis of C. elegans as a model for temperature perception, neural information processing and neural plasticity", Worm, 1(1), pp. 31-41, 2012.
  5. Han, B., Kim, D., Ko, U. H., & Shin, J. H., A sorting strategy for C. elegans based on sizedependent motility and electrotaxis in a microstructured channel. Lab Chip, 12, 4128-34, 2012.
  6. Wang, Z., I. Lee, T. J. Jeon and S. M. Kim, "Micro-/Nanofluidic Device for Tunable Generation of a Concentration Gradient: Application to Caenorhabditis Elegans Chemotaxis." Anal Bioanal Chem 406, no. 11 : 2679-86, 2014.
  7. Jurado, P., E. Kodama, Y. Tanizawa and I. Mori. "Distinct Thermal Migration Behaviors in Response to Different Thermal Gradients in Caenorhabditis Elegans." Genes Brain and Behavior 9, no. 1, 120-127, 2010.
  8. Tanizawa, Y., A. Kuhara, H. Inada, E. Kodama, T. Mizuno and I. Mori. "Inositol Monophosphatase Regulates Localization of Synaptic Components and Behavior in the Mature Nervous System of C-Elegans." Genes & Development 20, no. 23, 3296-3310, 2006.
  9. Xu, Z., Li, H., & Wadsworth, W. G., "The roles of multiple UNC-40 (DCC) receptor-mediated signals in determining neuronal asymmetry induced by the UNC-6 (netrin) ligand." Genetics, 183, 941-9, 2009.
  10. Dibb, N. J., Brown, D. M., Karn, J., Moerman, D. G., Bolten, S. L., & Waterston, R. H., "Sequence analysis of mutations that affect the synthesis, assembly and enzymatic activity of the unc-54 myosin heavy chain of Caenorhabditis elegans." J Mol Biol, 183, 543-51, 1985.