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A Study on the Single-dose Oral Toxicity of Super Key in Sprague-Dawley Rats

  • Kim, Jinhee (Department of Korean Rehabilitation Medicine, Wonkwang University Gwangju Korean Medical Hospital) ;
  • Lee, Jongcheol (Department of Acupuncture & Moxibustion Medicine, Wonkwang University Gwangju Korean Medical Hospital) ;
  • Kim, Sungchul (Department of Acupuncture & Moxibustion Medicine, Wonkwang University Gwangju Korean Medical Hospital)
  • Received : 2015.03.19
  • Accepted : 2015.07.21
  • Published : 2015.09.30

Abstract

Objectives: This study was performed to analyze the single-dose oral toxicity of the super key (processed sulfur). Methods: All experiments were conducted at Medvill, an institution authorized to perform non-clinical studies, under the Good Laboratory Practice (GLP) regulations. In order to investigate the oral toxicity of super key. We administered it orally to Sprague-Dawley (SD) rats. The SD rats were divided into four groups of five male and five female animals per group: group 1 being the control group and groups 2, 3, and 4 being the experimental groups. Doses of super key 500 mg/kg, 1,000 mg/kg and 2,000 mg/kg were administered to the experimental groups, and a dose of normal saline solution, 10 mL/kg, was administered to the control group. We examined the survival rates, weights, clinical signs, gross findings and necropsy findings. This study was conducted under the approval of the Institutional Animal Ethics Committee. (Approval number: A01-14018). Results: No deaths or abnormalities occurred in any of the four groups. Although slight decreases in the weights of some female rats were noted, no significant changes in weights or differences in the gross findings between the control group and the experimental groups were observed. To check for abnormalities in organs, we used microscopy to examine representative histological sections of each specified organ; the results showed no significant differences in any of the organs. Conclusion: The results of this research showed that administration of 500 - 2,000 mg/kg of super key did not cause any changes in the weights or in the results of necropsy examinations. Neither did it result in any mortalities. The above findings suggest that treatment with super key is relatively safe. Further studies on this subject are needed to yield more concrete evidence.

Acknowledgement

Supported by : Korea Health Industry Development Institute (KHIDI)

References

  1. Shin HM. Repeated dose 4-week oral-treatment for DRF toxicity test of HMC05 in sprague-dawley rats. J Korean Oriental Med. 2009;30(5):102-14.
  2. Lee MY, Seo CS, Kim JY, Shin HK. Genotoxicity evaluation of guibi-tang extract using an in vitro bacterial reverse mutation assay, chromosome aberration assay, and in vivo micronucleus test. BMC Complement Altern Med. 2014;14(1):215-22. https://doi.org/10.1186/1472-6882-14-215
  3. Yuan JB, Huang Q, Ren G, Shi M, Chen L, Yang WL, et al. Acute and subacute toxicity of the extract of Aristolochiae fructus and honey-fried Aristolochiae fructus in rodents. Biol Pharm Bull. 2014;37(3):387-93. https://doi.org/10.1248/bpb.b13-00736
  4. Ingenbleek Y. The nutritional relationship linking sulfur to nitrogen in living organisms. J Nutr. 2006;136(6):1641-51. https://doi.org/10.1093/jn/136.6.1641S
  5. Heo J. [Dongeui science institute, treasured mirror of eastern medicine (東醫寶鑑)]. Seoul: Bubin publishers; 2007. p. 2035. Korean.
  6. Pruksachatkunakorn C, Damrongsak M, Sinthupuan S. Sulfur for scabies outbreaks in orphanages. Pediatr Dermatol. 2002;19(5):448-53. https://doi.org/10.1046/j.1525-1470.2002.00205.x
  7. Kenawi MZ, Morsy TA, Abdalla KF, El Hady HM. Treatment of human scabies by sulfur and permethrin. J Egypt Soc Parasitol. 1993;23(3):691-6.
  8. Gupta AK, Nicol K. The use of sulfur in dermatology. J Drugs Dermatol. 2003;3(4):427-31.
  9. Kong CS, Bak SS, Rhee SH, Rho CW, Kim NK, Choi KL, et al. Fermentation properties of young radish kimchi prepared using young radish cultivated in the soil containing sulfur and it's inhibitory effect on the growth of AGS human gastric adenocarcinoma cells. J Korean Soc Food Sci Nutr. 2006;35(2):158-63. https://doi.org/10.3746/jkfn.2006.35.2.158
  10. Bak SS, Kong CS, Rhee SH, Rho CW, Kim NK, Choi KL, et al. Effect of sulfur enriched young radish kimchi on the induction of apoptosis in HT-29 human colon cancer cells. J Food Sci Nutr. 2006;11(3):184-90. https://doi.org/10.3746/jfn.2006.11.3.184
  11. Choi GH, Kim CH. Growth inhibition of extract from sulfur fed duck carcass against various cancer cell lines. Korean J Food Sci Anim Resour. 2002;22(4):348-51.
  12. Ha AW, Hong KH, Kim HS, Kim WK. Inorganic sulfur reduces cell proliferation by inhibiting of ErbB2 and ErbB3 protein and mRNA expression in MDA-MB-231 human breast cancer cells. Nutr Res Pract. 2013;7(2):89-95. https://doi.org/10.4162/nrp.2013.7.2.89
  13. Duan F, Li Y, Chen L, Zhou X, Chen J, Chen H, et al. Sulfur inhibits the growth of androgen-independent prostate cancer in vivo. Oncol Lett. 2015;9(1):437-41. https://doi.org/10.3892/ol.2014.2700
  14. Parcell S. Sulfur in human nutrition and applications in medicine. Altern Med Rev. 2002;7(1):22-44.
  15. Kim YK. [The principle and test method. toxicology]. Seoul: Donghwagisul; 1994. p. 15-8. Korean.
  16. Kim YG. [Toxicology]. Seoul: Donghwagisul; 1984. p. 15-8. Korean.
  17. Korea Food & Drug Administration. Korea Food & Drug Administration notification [Internet]. Seoul: Korea Food & Drug Administration; 2005 [cited 2013 March 1]. Available from: http://lawwizice.wordpress.com/.