DOI QR코드

DOI QR Code

Isolation and identification of canine parvovirus type 2b in Korean dogs

  • Yang, Dong-Kun (Viral disease division, Animal and Plant Quarantine Agency) ;
  • Kang, Kyung-Suk (Viral disease division, Animal and Plant Quarantine Agency) ;
  • Jo, Hyun-Ye (Viral disease division, Animal and Plant Quarantine Agency) ;
  • Kim, Ha-Hyun (Viral disease division, Animal and Plant Quarantine Agency) ;
  • Choi, Sung-Suk (Viral disease division, Animal and Plant Quarantine Agency) ;
  • Song, Jae-Young (Viral disease division, Animal and Plant Quarantine Agency)
  • Received : 2015.04.21
  • Accepted : 2015.07.17
  • Published : 2015.09.30

Abstract

Canine parvovirus (CPV) is a major diarrhea-causing agent in puppies. Since CPV type 2 (CPV-2) emerged in 1978, new antigenic variants including CPV-2a, CPV-2b, and CPV-2c have been identified in many countries. Two puppies died suddenly at a veterinary clinic in Gyeonggi province, South Korea. Two viruses were isolated in A72 cells, confirmed as CPV strains based on a CPV rapid kit and an indirect fluorescence test and designated QIACP1403 and QIACP1404. The nucleotide sequences of complete VP2 genes of QIACP1403 and QIACP1404 were determined, and the corresponding amino acid sequences were deduced. Molecular analyses revealed that the QIACP1403 and QIACP1404 isolates were type CPV-2b. Several mutated amino acids were detected on VP2 gene residues of the two isolates. Phylogenetic analyses showed that the two isolates were most closely related to strain CPV-BM11, which was isolated from Chinese dogs in 2011. Our results suggest that these isolates may be a candidate for a vaccine to prevent CPV infection in dogs after conducting passages of the isolates in an in vitro culture system.

Acknowledgement

Supported by : Animal and Plant Quarantine Agency

References

  1. Appel MJ, Cooper BJ, Greisen H, Scott F, Carmichael LE. Canine viral enteritis. I. Status report on corona- and parvo-like viral enteritides. Cornell Vet 1979, 69, 123-133.
  2. Battilani M, Scagliarini A, Tisato E, Turilli C, Jacoboni I, Casadio R, Prosperi S. Analysis of canine parvovirus sequences from wolves and dogs isolated in Italy. J Gen Virol 2001, 82, 1555-1560. https://doi.org/10.1099/0022-1317-82-7-1555
  3. Buonavoglia C, Martella V, Pratelli A, Tempesta M, Cavalli A, Buonavoglia D, Bozzo G, Elia G, Decaro N, Carmichael L. Evidence for evolution of canine parvovirus type 2 in Italy. J Gen Virol 2001, 82, 3021-3025. https://doi.org/10.1099/0022-1317-82-12-3021
  4. Carmichael LE. An annotated historical account of canine parvovirus. J Vet Med B Infect Dis Vet Public Health 2005, 52, 303-311. https://doi.org/10.1111/j.1439-0450.2005.00868.x
  5. Chen XY, Xie ZJ, Zhao ZP, Jiang SJ, Zhao HK, Zhu YL, Zhang XX. Genetic diversity of parvovirus isolates from dogs and wild animals in China. J Wildl Dis 2011, 47, 1036-1039. https://doi.org/10.7589/0090-3558-47.4.1036
  6. Cotmore SF, Tattersall P. Parvovirus diversity and DNA damage responses. Cold Spring Harb Perspect Biol 2013, 5, a012989.
  7. Decaro N, Buonavoglia C. Canine parvovirus-a review of epidemiological and diagnostic aspects, with emphasis on type 2c.Vet Microbiol 2012, 155, 1-12. https://doi.org/10.1016/j.vetmic.2011.09.007
  8. Decaro N, Desario C, Addie DD, Martella V, Vieira MJ, Elia G, Zicola A, Davis C, Thompson G, Thiry E, Truyen U, Buonavoglia C. The study molecular epidemiology of canine parvovirus, Europe. Emerg Infect Dis 2007, 13, 1222-1224. https://doi.org/10.3201/eid1308.070505
  9. Frolich K, Streich WJ, Fickel J, Jung S, Truyen U, Hentschke J, Dedek J, Prager D, Latz N. Epizootiologic investigations of parvovirus infections in free-ranging carnivores from Germany. J Wildl Dis. 2005, 41, 231-235. https://doi.org/10.7589/0090-3558-41.1.231
  10. Hirayama K, Kano R, Hosokawa-Kanai T, Tuchiya K, Tsuyama S, Nakamura Y, Sasaki Y, Hasegawa A. VP2 gene of a canine parvovirus isolate from stool of a puppy. J Vet Med Sci. 2005, 67, 139-143. https://doi.org/10.1292/jvms.67.139
  11. Hong C, Decaro N, Desario C, Tanner P, Pardo MC, Sanchez S, Buonavoglia C, Saliki JT. Occurrence of canine parvovirus type 2c in the United States. J Vet Diagn Invest 2007, 19, 535-539. https://doi.org/10.1177/104063870701900512
  12. Jeoung SY, Ahn SJ, Kim D. Genetic analysis of VP2 gene of canine parvovirus isolates in Korea. J Vet Med Sci. 2008, 70, 719-722. https://doi.org/10.1292/jvms.70.719
  13. Kang BK, Song DS, Lee CS, Jung KI, Park SJ, Kim EM, Park BK. Prevalence and genetic characterization of canine parvoviruses in Korea. Virus Genes 2008, 36, 127-133. https://doi.org/10.1007/s11262-007-0189-6
  14. Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson TJ, Higgins DG. Clustal W and Clustal X version 2.0. Bioinformatics 2007, 23, 2947-2948. https://doi.org/10.1093/bioinformatics/btm404
  15. Park SA, Park SY, Song CS, Choi IS, Kim HY, Lee JB, Lee NH. Development of a novel vaccine against canine parvovirus infection with a clinical isolate of the type 2b strain. Clin Exp Vaccine Res 2012, 1, 70-76. https://doi.org/10.7774/cevr.2012.1.1.70
  16. Parrish CR. Host range relationships and the evolution of canine parvovirus. Vet Microbiol 1999, 69, 29-40. https://doi.org/10.1016/S0378-1135(99)00084-X
  17. Pedroza-Roldan C, Paez-Magallan V, Charles-Nino C, Elizondo-Quiroga D, Leonel De Cervantes-Mireles R, Lopez-Amezcua MA. Genotyping of Canine parvovirus in western Mexico. J Vet Diagn Invest 2015, 27, 107-111. https://doi.org/10.1177/1040638714559969
  18. Pratelli A, Moschidou P. Host range of canine minute virus in cell culture. J Vet Diagn Invest 2012, 24, 981-985. https://doi.org/10.1177/1040638712453579
  19. Senda M, Hirayama N, Itoh O, Yamamoto H. Canine parvovirus: strain difference in haemagglutination activity and antigenicity. J Gen Virol 1988, 69, 349-354. https://doi.org/10.1099/0022-1317-69-2-349
  20. Strassheim ML, Gruenberg A, Veijalainen P, Sgro JY, Parrish CR. Two dominant neutralizing antigenic determinants of canine parvovirus are found on the threefold spike of the virus capsid. Virology 1994, 198, 175-184. https://doi.org/10.1006/viro.1994.1020
  21. Tamura K, Dudley J, Nei M, Kumar S. MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 2007, 24, 1596-1599. https://doi.org/10.1093/molbev/msm092
  22. Wang HC, Chen WD, Lin SL, Chan JP, Wong ML. Phylogenetic analysis of canine parvovirus VP2 gene in Taiwan. Virus Genes 2005, 31, 171-174. https://doi.org/10.1007/s11262-005-1791-0
  23. Yang DK, Kim BH, Kim YH, Lee KW, Choi SS, Son SW. Genetic analysis of canine parvovirus vaccine strains in Korea. Korean J Vet Res 2009, 49, 243-248.