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Serosurveillance and establishment of a reverse transcription-polymerase chain reaction assay for bovine parainfluenza virus type 5

  • Yang, Dong-Kun (Viral disease division, Animal and Plant Quarantine Agency) ;
  • Choi, Sung-Suk (Viral disease division, Animal and Plant Quarantine Agency) ;
  • Lee, Beom-Joo (Viral disease division, Animal and Plant Quarantine Agency) ;
  • Kim, Ha-Hyun (Viral disease division, Animal and Plant Quarantine Agency) ;
  • Jo, Hyun-Ye (Viral disease division, Animal and Plant Quarantine Agency)
  • Received : 2015.06.20
  • Accepted : 2015.08.24
  • Published : 2015.09.30

Abstract

Bovine parainfluenza virus type 5 (bPIV5) was isolated from cattle with downer cow syndrome in 2012, and included both respiratory and neurotropic pathogens from a variety of animals. In the current study, we conducted serosurveillance using sera obtained from seven Korean farms and optimized a reverse transcription-polymerase chain reaction (RT-PCR) assay to detect bPIV5. The overall seropositive rate for Korean cattle was 21.4% (163/760). A farm located near the city of Milyang in Gyeoungnam province had a markedly elevated seropositive rate for bPIV5 compared to that of the other six farms. The regional seropositive rates were 4.2% (8/192) for Haman, 19.5% (18/55) for Hwasung, 73.9% (65/88) for Milyang, 26.0% (50/192) for Namwon, 1.0% (1/96) for Uljin, 13.5% (13/96) for Yeongju, and 32.7% (8/41) for Yongin. The sensitivity and specificity of three RT-PCR primer sets used to amplify the conserved fusion gene of bPIV5 were also evaluated. An RT-PCR assay using the bPIVFR3 primer set was 10-fold more sensitive than the assays using the two other primer sets and did not result in non-specific amplification. These results demonstrated that the bPIFR3 primer set can be used to detect bPIV5.

Acknowledgement

Supported by : Animal, and Plant Quarantine Agency

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