Serosurveillance and establishment of a reverse transcription-polymerase chain reaction assay for bovine parainfluenza virus type 5

  • Yang, Dong-Kun (Viral disease division, Animal and Plant Quarantine Agency) ;
  • Choi, Sung-Suk (Viral disease division, Animal and Plant Quarantine Agency) ;
  • Lee, Beom-Joo (Viral disease division, Animal and Plant Quarantine Agency) ;
  • Kim, Ha-Hyun (Viral disease division, Animal and Plant Quarantine Agency) ;
  • Jo, Hyun-Ye (Viral disease division, Animal and Plant Quarantine Agency)
  • Received : 2015.06.20
  • Accepted : 2015.08.24
  • Published : 2015.09.30


Bovine parainfluenza virus type 5 (bPIV5) was isolated from cattle with downer cow syndrome in 2012, and included both respiratory and neurotropic pathogens from a variety of animals. In the current study, we conducted serosurveillance using sera obtained from seven Korean farms and optimized a reverse transcription-polymerase chain reaction (RT-PCR) assay to detect bPIV5. The overall seropositive rate for Korean cattle was 21.4% (163/760). A farm located near the city of Milyang in Gyeoungnam province had a markedly elevated seropositive rate for bPIV5 compared to that of the other six farms. The regional seropositive rates were 4.2% (8/192) for Haman, 19.5% (18/55) for Hwasung, 73.9% (65/88) for Milyang, 26.0% (50/192) for Namwon, 1.0% (1/96) for Uljin, 13.5% (13/96) for Yeongju, and 32.7% (8/41) for Yongin. The sensitivity and specificity of three RT-PCR primer sets used to amplify the conserved fusion gene of bPIV5 were also evaluated. An RT-PCR assay using the bPIVFR3 primer set was 10-fold more sensitive than the assays using the two other primer sets and did not result in non-specific amplification. These results demonstrated that the bPIFR3 primer set can be used to detect bPIV5.


Supported by : Animal, and Plant Quarantine Agency


  1. Aguilar JC, Perez-Brena MP, Garcia ML, Cruz N, Erdman DD, Echevarria JE. Detection and identification of human parainfluenza viruses 1, 2, 3, and 4 in clinical samples of pediatric patients by multiplex reverse transcription-PCR. J Clin Microbiol 2000, 38, 1191-1195.
  2. Ajiki M, Takamura K, Hiramatsu K, Nakai M, Sasaki N, Konishi S. Isolation and characterization of parainfluenza 5 virus from a dog. Nihon Juigaku Zasshi 1982, 44, 607-618.
  3. Appel MJ, Percy DH. SV-5-like parainfluenza virus in dogs. J Am Vet Med Assoc 1970, 156, 1778-1781.
  4. Bellau-Pujol S, Vabret A, Legrand L, Dina J, Gouarin S, Petitjean-Lecherbonnier J, Pozzetto B, Ginevra C, Freymuth F. Development of three multiplex RT-PCR assays for the detection of 12 respiratory RNA viruses. J Virol Methods 2005, 126, 53-63.
  5. Chatziandreou N, Stock N, Young D, Andrejeva J, Hagmaier K, McGeoch DJ, Randall RE. Relationships and host range of human, canine, simian and porcine isolates of simian virus 5 (parainfluenza virus 5). J Gen Virol 2004, 85, 3007-3016.
  6. Chen Z, Xu P, Salyards GW, Harvey SB, Rada B, Fu ZF, He B. Evaluating a parainfluenza virus 5-based vaccine in a host with pre-existing immunity against parainfluenza virus 5. PLoS One 2012, 7, e50144.
  7. Ellis J, Anseeuw E, Gow S, Bryan H, Salb A, Goji N, Rhodes C, La Coste S, Smits J, Kutz S. Seroepidemiology of respiratory (group 2) canine coronavirus, canine parainfluenza virus, and Bordetella bronchiseptica infections in urban dogs in a humane shelter and in rural dogs in small communities. Can Vet J 2011, 52, 861-868.
  8. Goswami KK, Lange LS, Mitchell DN, Cameron KR, Russell WC. Does simian virus 5 infect humans? J Gen Virol 1984, 65, 1295-1303.
  9. Johnson JB, Capraro GA, Parks GD. Differential mechanisms of complement-mediated neutralization of the closely related paramyxoviruses simian virus 5 and mumps virus. Virology 2008, 376, 112-123.
  10. Julkunen I. Serological diagnosis of parainfluenza virus infections by enzyme immunoassay with special emphasis on purity of viral antigens. J Med Virol 1984, 14, 177-187.
  11. Lamb RA, Parks GD. Paramyxoviridae: The viruses and their replication. In: Knipe DM, Howley PM (eds.). Fields Virology. 5th ed. pp. 1449-1496. Lippincott Williams & Wilkins/Wolters Kluwer Business, Philadelphia, 2007.
  12. Lamb RA, Paterson RG, Jardetzky TS. Paramyxovirus membrane fusion: lessons from the F and HN atomic structures. Virology 2006, 344, 30-37.
  13. Lee YN, Lee C. Complete genome sequence of a novel porcine parainfluenzavirus 5 isolate in Korea. Arch Virol 2013, 158, 1765-1772.
  14. Randall RE, Young DF. Comparison between parainfluenza virus type 2 and simian virus 5: monoclonal antibodies reveal major antigenic differences. J Gen Virol 1988, 69, 2051-2060.
  15. Schmitt AP, He B, Lamb RA. Involvement of the cytoplasmic domain of the hemagglutinin-neuraminidase protein in assembly of the paramyxovirus simian virus 5. J Virol 1999, 73, 8703-8712.
  16. Stott EJ, Thomas LH, Collins AP, Crouch S, Jebbett J, Smith GS, Luther PD, Caswell R. A survey of virus infections of the respiratory tract of cattle and their association with disease. J Hyg (Lond) 1980, 85, 257-270.
  17. Sun D, Luthra P, Xu P, Yoon H, He B. Identification of a phosphorylation site within the P protein important for mRNA transcription and growth of parainfluenza virus 5. J Virol 2011, 85, 8376-8385.
  18. Syrmis MW, Whiley DM, Thomas M, Mackay IM, Williamson J, Siebert DJ, Nissen MD, Sloots TP. A sensitive, specific, and cost-effective multiplex reverse transcriptase-PCR assay for the detection of seven common respiratory viruses in respiratory samples. J Mol Diagn 2004, 6, 125-131.
  19. van der Logt JT, Heessen FW, van Loon AM, van der Veen J. Hemadsorption immunosorbent technique for determination of mumps immunoglobulin M antibody. J Clin Microbiol 1982, 15, 82-86.
  20. Yang DK, Nah JJ, Kim HH, Choi SS, Bae YC, Park JW, Song JY. Isolation of novel bovine parainfluenza virus type 5 (bPIV5) and its incidence in Korean cattle. Korean J Vet Res 2014, 54, 107-112.
  21. Yin HS, Wen X, Paterson RG, Lamb RA, Jardetzky TS. Structure of the parainfluenza virus 5 F protein in its metastable, prefusion conformation. Nature 2006, 439, 38-44.
  22. Zuffa T, Krobot P. Detection of antibodies against infectious viral laryngotracheitis and parainfluenza 2 in dogs bred in Czechoslovakia. Vet Med (Praha) 1987, 32, 689-694.