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Virulence factors, antimicrobial resistance patterns, and genetic characteristics of hydrogen sulfide-producing Escherichia coli isolated from swine

  • Park, Hyun-Eui (Department of Infectious Disease, College of Veterinary Medicine, Seoul National University) ;
  • Shin, Min-Kyoung (Department of Infectious Disease, College of Veterinary Medicine, Seoul National University) ;
  • Park, Hong-Tae (Department of Infectious Disease, College of Veterinary Medicine, Seoul National University) ;
  • Shin, Seung Won (Department of Infectious Disease, College of Veterinary Medicine, Seoul National University) ;
  • Jung, Myunghwan (Department of Infectious Disease, College of Veterinary Medicine, Seoul National University) ;
  • Im, Young Bin (Department of Infectious Disease, College of Veterinary Medicine, Seoul National University) ;
  • Yoo, Han Sang (Department of Infectious Disease, College of Veterinary Medicine, Seoul National University)
  • Received : 2015.01.10
  • Accepted : 2015.08.27
  • Published : 2015.09.30

Abstract

Escherichia (E.) coli is commensal bacteria found in the intestine; however, some pathogenic strains cause diseases in animals and humans. Although E. coli does not typically produce hydrogen sulfide ($H_2S$), $H_2S$-producing strains of E. coli have been identified worldwide. The relationship between virulence and $H_2S$ production has not yet been determined. Therefore, characteristics of $H_2S$-producing isolates obtained from swine feces were evaluated including antibiotic resistance patterns, virulence gene expression, and genetic relatedness. Rates of antibiotic resistance of the $H_2S$-producing E. coli varied according to antibiotic. Only the EAST1 gene was detected as a virulence gene in five $H_2S$-producing E. coli strains. Genes conferring $H_2S$ production were not transmissible although the sseA gene encoding 3-mercaptopyruvate sulfurtransferase was detected in all $H_2S$-producing E. coli strains. Sequences of the sseA gene motif CGSVTA around Cys238 were also identical in all $H_2S$- producing E. coli strains. Diverse genetic relatedness among the isolates was observed by pulsed-field gel electrophoresis analysis. These results suggested that $H_2S$-producing E. coli strains were not derived from a specific clone and $H_2S$ production in E. coli is not associated with virulence genes.

Acknowledgement

Grant : Cooperative Research Program for Agriculture Science & Technology Development

Supported by : Rural Development Administration, Research Institute for Veterinary Sciences, Seoul National University

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