HaCaT 세포에서 회향 열매의 피부장벽기능과 hyaluronic acid 생성에 미치는 영향

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유학인;양인준;빅터루베리오린차;박인식;이동웅;신흥묵
Yu, Hak Yin;Yang, In Jun;Lincha, V.R;Park, In Sik;Lee, Dong-Ung;Shin, Heung Mook

  • 투고 : 2015.06.29
  • 심사 : 2015.08.13
  • 발행 : 2015.08.30

초록

회향은 미나리과에 속하는 다년생식물인 Foeniculum vulgare Mill.의 성숙한 과실로서 항염, 진통, 피부노화방지 등의 효과를 가지고 있어 다양한 질환에 우수한 치료효과를 나타내는 것으로 알려져 왔다. 따라서 본 연구에서는 기능성이 우수한 회향 열매 추출물을 이용하여 피부 질환 치료제 및 피부장벽 기능 개선 소재로서의 적용가능성을 확인하였다. 이를 위해 각질형성세포주인 HaCaT에 회향 열매 메탄올 추출물과 그 분획물(hexane, methyl chloride, ethyl acetate, butanol)을 처리한 후, involucrin, loricrin, filaggrin의 발현과 hyaluronic acid의 생성 및 β-defensin -1, -2, -3, LL-37의 mRNA 발현을 측정하였다. 그 결과butanol 분획물 50 μg/ml을 24시간 동안 처리시 involucrin과 filaggrin단백질 발현을 각각122.8%, 105%로 유의하게 증가시킴을 확인하였다. Elisa assay를 통해 분석한 결과, ethyl acetate와 butanol 분획물은 대조군에 비해 hyaluronic acid의 생성을 각각17%, 11% 증가시켰으며, 이는 hyaluronic acid synthesis-1의 mRNA 발현 증가에 의한 것임을 확인하였다. 그러나 회향 열매 메탄올 추출물과 분획물 모두에서 β-defensin -1, -2, -3, LL-37의 mRNA 발현은 증가되지 않았다. 이러한 결과는 회향 열매 butanol 분획물이 각질형성세포에서 물리적 장벽을 형성하고 보습인자 조절을 통해 피부장벽 기능을 강화하는데 효과적임을 의미한다.

키워드

Cornified envelopment peptide;Foeniculum vulgare;HaCaT cell;hyaluronic acid;skin barrier function

참고문헌

  1. Song, H. J., Jin, M. H. and Lee, S. H. 2013. Effect of ferulic acid isolated from Cnidium officinale on the synthesis of hyaluronic acid. J. Soc. Cosmet. Scientists Kor. 39, 281-288. https://doi.org/10.15230/SCSK.2013.39.4.281
  2. Steinert, P. M. 1995. A model for the hierarchical structure of the human epidermal cornified cell envelope. Cell Death Differ. 2, 33-40.
  3. Steinert, P. M. and Marekov, L. N. 1995. The poteins elafin, filaggrin, keratin intermediate filaments, loricrin, and small proline-rich proteins 1 and 2 are isodipeptide cross-linked components of the human epidermal cornified cell envelope. J. Biol. Chem. 270, 17702-17711. https://doi.org/10.1074/jbc.270.30.17702
  4. Steven, A. C. and Steiner, P. M. 1994. Protein composition of cornified cell envelopes of epidermal keratinocyte. J. Cell Sci. 107, 693-700.
  5. Taïieb, A. 1999. Hypothesis: from epidermal barrier dysfunction to atopic disorders. Contact. Dermatitis. 41, 177-180. https://doi.org/10.1111/j.1600-0536.1999.tb06125.x
  6. Thyssen, J. P., Ross-Hansen, K., Johansen, J. D., Zachariae, C., Carlsen, B. C., Linneberg, A., Bisqaard, H., Carson, C. G., Nielsen, N. H., Meldqaard, M., Szecsi, P. B., Stender, S. and Menné, T. 2012. Filaggrin loss-of-function mutation R501X and 2282del4 carrier status is associated with fissured skin on the hands: results from a cross-sectional population study. Br. J. Dermatol. 166, 46-53. https://doi.org/10.1111/j.1365-2133.2011.10530.x
  7. Yamasaki, K. and Gallo, R. L. 2008. Antimicrobial peptides in human skin disease. Eur. J. Dermatol. 18, 11-21.
  8. Reinholz, M., Ruzicka, T. and Schauber, J. 2012. Cathelicidin LL-37: an antimicrobial peptide with a role in inflammatory skin disease. Ann. Dermatol. 24, 126-135. https://doi.org/10.5021/ad.2012.24.2.126
  9. Ruberto, G., Baratta, M. T., Deans, S. J. and Dorman, H. J. 2000. Antioxidant and antimicrobial activity of Foeniculum vulgare and Crithmum maritimum essential oils. Planta. Med. 66, 687-693. https://doi.org/10.1055/s-2000-9773
  10. Sakai, S., Sayo, T., Kodama, S. and Inoue, S. 1999. NMethyl-L-serine stimulates hyaluronan production in human skin fibroblasts. Skin Pharmacol. Appl. Skin Physiol. 12, 276-283. https://doi.org/10.1159/000066253
  11. Schroder, J. M. and Harder, J. 2006. Antimicrobial skin peptides and proteins. Cell. Mol. Life Sci. 63, 469-486. https://doi.org/10.1007/s00018-005-5364-0
  12. Seo, D. J., Kim, T. H., Kim, H. S. and Choe, M. 2011. Effects of Foeniculi fructus water extracts on activities of key enzymes of lipid metabolism related with obesity. Kor. J. Plant Res. 24, 181-188 https://doi.org/10.7732/kjpr.2011.24.2.181
  13. Shin, M. G. 2000. Clinical traditional herbalogy, pp. 305-306, Young-lim Publishing, Seoul, Korea.
  14. Singh, G., Maurya, S., De Lampasona, M. P. and Catalan, C. 2006. Chemical constituents, antifungal and antioxidative potential of Foeniculum vulgare volatile oil and its acetone extract. Food Control 17, 745-752. https://doi.org/10.1016/j.foodcont.2005.03.010
  15. Smith, F. J., Irvine, A. D., Terron-Kwiatkowski, A., Sandilands, A., Campbell, L. E., Zhao, Y., Liao, H., Evans, A. T., Goudie, D. R., Lewis-Jones, S., Arseculeratne, G., Munro, C. S., Sergeant, A., O’Regan, G., Bale, S. J., Compton, J. G., DiGiovanna, J. J., Presland, R. B., Fleckman, P. and McLean, W. H. 2006. Loss-of-function mutations in the gene encoding filaggrin cause ichthyosis vulgaris. Nat. Genet. 38, 337-342. https://doi.org/10.1038/ng1743
  16. Longas,M. O., Russell, C. S. and He, X. Y. 1987. Evidence for structural changes in dermatan sulfate and hyaluronic acid with aging. Carbohydr. Res. 159, 127-136. https://doi.org/10.1016/S0008-6215(00)90010-7
  17. Malaisse, J., Bourguignon, V., De Vuyst, E., Lambert de Rouvroit C., Nikkels, A. F., Flamion, B. and Poumay, Y. 2014. Hyaluronan metabolism in human keratinocytes and atopic dermatitis skin is driven by a balance of hyaluronan synthases 1 and 3. J. Invest. Dermatol. 134, 2174-2182. https://doi.org/10.1038/jid.2014.147
  18. Mohsenzadeh, M. 2007. Evaluation of antibacterial activity of selected Iranian essential oils against Staphylococcus aureus and Escherichia coli in nutrient broth medium. Pak. J. Biol. Sci. 10, 3693–3697. https://doi.org/10.3923/pjbs.2007.3693.3697
  19. Nemes, Z. and Steinert, P. M. 1999. Brick and mortar of the epidermal barrier. Exp. Mol. Med. 31, 5-19. https://doi.org/10.1038/emm.1999.2
  20. Oktay, M., Gülcin, I. and Küfrevioğlu, Ö. İ. 2003. Determination of in vitro antioxidant activity of funnel (Foeniculum vulgare) seed extracts. LWT-Food Sci. Technol. 36, 263-271. https://doi.org/10.1016/S0023-6438(02)00226-8
  21. Ong, P. Y., Ohtake, T., Brandt, C., Strickland, I., Boguniewicz, M., Ganz, T., Gallo, R. and Leung, D. Y. 2002. Endogenous antimicrobial peptides and skin infections in atopic dermatitis. N. Engl. J .Med. 347, 1151-1160. https://doi.org/10.1056/NEJMoa021481
  22. Pienimäki, J. P., Rilla, K., Fülöp, C., Sironen, R. K., Karvinen, S., Pasonen, S., Lammi, M. J., Tammi, R., Hascall V. C. and Tammi, M. I. 2001. Epidermal growth factor activates hyaluronan synthase 2 in epidermal keratinocytes and increases pericellular and intracellular hyaluronan. J. Biol. Chem. 276, 20428-20435. https://doi.org/10.1074/jbc.M007601200
  23. Rasul, A., Akhtar, N., Khan, B. A., Mahmood, T., Uz Zaman, S. and Khan, H. M. 2012. Formulation development of a cream containing fennel extract: in vivo evaluation for anti-aging effects. Pharmazie 67, 54-58.
  24. Ishida-Yamamoto, A., Kartasova, T., Matsuo, S., Kuroki, T. and Izuka, H. 1997. Involucrin and SPRR are synthasized sequentially in differentiating cultured epidermal cells. J. Invest. Dermatol. 108, 12-16. https://doi.org/10.1111/1523-1747.ep12285611
  25. Izadpahah, A. and Gallo, R. L. 2005. Antimicrobial peptides. J. Am. Acad. Dermatol. 52, 381-390. https://doi.org/10.1016/j.jaad.2004.08.026
  26. Karvinen, S., Pasonen-Seppänen, S., Hyttinen, J. M., Pienimäki, J. P., Törrönen, K., Jokela, T. A., Tammi, M. I. and Tammi, R. 2003. Keratinocyte growth factor stimulates migration and hyaluronan synthesis in the epidermis by activation of keratinocyte hyaluronan synthases 2 and 3. J. Biol. Chem. 278, 49495-49504. https://doi.org/10.1074/jbc.M310445200
  27. Kim, H. J., Shin, J. U. and Lee, K. H. 2013. Atopic dermatitis and skin barrier dysfunction. Allergy Asthma Respir. Dis. 1, 20-28. https://doi.org/10.4168/aard.2013.1.1.20
  28. Kim, S. M., Choi, C. H., Kim, J. W., Won, S. R. and Rhee, H. I. 2008. The anticaries activity of hot water extracts from Foeniculum vulgare. J. Kor. Soc. Appl. Biol. Chem. 51, 84-87
  29. Lee, J. C., Lee, E., Lee, Y. C., Oh, H., Yoon, H. S., Ha, T. K. and Hong, E. H. 2007. Effects of Fructus foeniculi extract on recovering liver function. Kor. J. Herbology. 22, 213-218
  30. Lee, J. J., Choe, M. S., Chung, C. S. and Choe, B. 2003. Effect of food restriction on rat adipotise lipoprotein lipase activity and lipogenesis. Kor. J. Exercise Nutr. 7, 135-141
  31. Hata, T. R. and Gallo, R. L. 2008. Antimicrobial peptides, skin infections and atopic dermatitis. Semin. Cutan. Med. Surg. 27, 144-150. https://doi.org/10.1016/j.sder.2008.04.002
  32. Hwang, H. Y., Choi, Y. S., Jung, B. H., Lim, B. W. and Kim, J. D. 2007. Effect of Foeniculum vulgare Gaertner extracts on blood pressure in spontaneously hypertensive rats. Proceedings of the Convention of the Korean Society of Applied Pharmacology. 161-162
  33. Forslin, B. 1994. A domain mosaic model of the skin barrier. Acta. Derrn. Venereol. 74, 1-6.
  34. Fuchs, E. 1993. Epidermal differntiation and keratin gene expression. J. Cell Sci. 17, 197-208.
  35. Gallo, R. L. and Huttner, K. M. 1998. Antimicrobial peptides: an emerging concept in cutaneous biology. J. Invest. Dermatol. 111, 739-743. https://doi.org/10.1046/j.1523-1747.1998.00361.x
  36. Gallo, R. L., Murakami, M., Ohtake, T. and Zaiou, M. 2002. Biology and clinical relevance of naturally occurring antimicrobial peptides. J. Allergy Clin. Immunol. 110, 823-831. https://doi.org/10.1067/mai.2002.129801
  37. Ganz, T. 1999. Defensins and host defense. Science 286, 420-421. https://doi.org/10.1126/science.286.5439.420
  38. Ghersetich, I., Notti, T., Gampainle, G., Grappone, C. and Dini, G. 1994. Hyalurinic acid in cutaneous intrinsic aging. Int. J. Dermatol. 33, 119-122. https://doi.org/10.1111/j.1365-4362.1994.tb01540.x
  39. Harding, C. R., Watkinson, A., Rawlings, A. V. and Scott, I. R. 2000. Dry skin, moisturization and corneodesmolysis. Int. J. Cosmet. Sci. 22, 21-52. https://doi.org/10.1046/j.1467-2494.2000.00001.x
  40. Hwang, S. P., Choi, U. H. and An, S. G. 2009. Skin barrier and antimicrobial activity. J. Skin Barrier Res. 11, 55-63.
  41. Howell, M. D., Kim, B. E., Gao, P., Grant, A. V., Boguniewicz, M., Debenedetto, A., Schneider, L., Beck, L. A., Barnes, K. C. and Leung, D. Y. 2007. Cytokine modulation of atopic dermatitis filaggrin skin expression. J. Allergy Clin. Immunol. 120, 150-155. https://doi.org/10.1016/j.jaci.2007.04.031
  42. Dübe, B., Lüke, H. J., Aumailley, M. and Prehm, P. 2001. Hyaluronan reduces migration and proliferation in CHO cells. Biochim. Biophys. Acta. 1538, 283-289. https://doi.org/10.1016/S0167-4889(01)00079-9
  43. Elias, P. M., Wood, L. C. and Feingold, K. R. 1999. Epidermal pathogenesis of inflammatory dermatoses. Am. J. Contact. Dermat. 10, 119-126.
  44. Anitha, T., Balakumar, C., Ilango, K. B., Benedict Jose, C. and Vetrivel, D. 2014. Antidiabetic activity of the aqueous extracts of Foeniculum vulgare on streptozotocin-induced diabetic rats. IJAPBC 3, 487-494.
  45. Bals, R. 2000. Epithelial antimicrobial peptides in host defense against infection. Respir. Res. 1, 141-150. https://doi.org/10.1186/rr25
  46. Braff, M. H., Bardan, A., Nizet, V. and Gallo, R. L. 2005. Cutaneous defense mechanisms by antimicrobial peptides. J. Invest. Dermatol. 125, 9-13. https://doi.org/10.1111/j.0022-202X.2004.23587.x
  47. Braff, M. H., Di Nardo, A. and Gallo, R. L. 2005. Keratinocytes store the antimicrobial peptide cathelicidin in lamellar bodies. J. Invest. Dermatol. 124, 394-400. https://doi.org/10.1111/j.0022-202X.2004.23443.x
  48. Brown, M. B. and Jones, S. A. 2005. Hyaluronic acid: a unique topical vehicle for the localized delivery of drugs to the skin. J. Eur. Acad. Dermatol. Venereol. 19, 308-318. https://doi.org/10.1111/j.1468-3083.2004.01180.x
  49. Choi, E. M. and Hwang, J. K. 2004. Antiinflammatory, analgesic and antioxidant activities of the fruit of Foeniculum vulgare. Fitoterapia 75, 557-565. https://doi.org/10.1016/j.fitote.2004.05.005
  50. Choi, E. M. and Koo, S. J. 2004. Inhibition of lipopolysaccharide-stimulated inflammatory mediator production in RAW264.7 macrophages by Foeniculum vulgare fruit extract. Kor. J. Food Cookery Sci. 20, 505-510.
  51. Cork, M. J., Danby, S. G., Vasilopoulos, Y., Hadgraft, J., Lane, M. E., Moustafa, M., Guy, R. H., Macqowan, A. L., Tazi-Ahnini, R. and Ward, S. J. 2009. Epidermal barrier dysfunction in atopic dermatitis. J. Invest. Dermatol. 129, 1892-1908. https://doi.org/10.1038/jid.2009.133
  52. Dahiya, P. and Kamal, R. 2013. Hyaluronic acid: a boon in periodontal therapy. N. Am. J. Med. Sci. 5, 309-315 https://doi.org/10.4103/1947-2714.112473
  53. Alis, R. S., Falconer, A., Ikram, M., Bissett, C. E., Cerio, R. and Quinn, A. G. 2001. Expression of the peptide antibiotics human beta defensin-1 and human beta defensin-2 in normal human skin. J. Invest. Dermatol. 117, 106-111. https://doi.org/10.1046/j.0022-202x.2001.01401.x

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