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Hyaluronic acid의 첨가방법에 따른 하이드로겔 콘택트렌즈의 물리적 특성과 lysozyme 흡착량 비교

Development of Hyaluronic Acid-Functionalized Hydrogel Lens and Characterization of Physical Properties and Lysozyme Adsorption

  • 투고 : 2015.08.06
  • 심사 : 2015.09.02
  • 발행 : 2015.09.30

초록

목적: 하이드로겔 콘택트렌즈에 HA(hyaluronic acid)를 IPN(interpenetrating polymer network)방법과 화학적 공유결합 방법(CCB; Chemical covalent bonding)으로 첨가하였을 때 광투과율과 함수율의 물리적 특성과 lysozyme 흡착량을 비교하여 하이드로겔 콘택트렌즈의 기능성 부여를 위한 효과적인 HA의 첨가방법을 살펴보고자 한다. 방법: 실험실에서 제조한 하이드로겔 콘택트렌즈에 IPN과 CCB의 두 가지 방법으로 HA를 첨가하였다. 광투과율은 300~800 nm 범위에서 측정하였으며, 함수율은 0.9% NaCl 생리 식염수를 사용하여 중량측정법으로 측정하였다. Lysozyme 흡착량은 lysozyme이 포함된 인공눈물을 제조하여 12시간동안 흡착시킨 후 HPLC로 정량하였다. 결과: HA가 첨가된 하이드로겔 콘택트렌즈의 함수율은 HA가 첨가되지 않은 렌즈에 비해 증가하였으며, CCB 방법에 비해 IPN 결합에서 함수율이 더 높게 나타났다. 광투과율은 HA 첨가 전과 후 모두 90% 이상으로 나타났다. Lysozyme 흡착 감소율은 IPN 방법으로 제조한 콘택트렌즈는 60.0%이며, CCB 방법으로 제조한 콘택트렌즈에서는 40.4%로 나타났다. 결론: CCB 방법은 기능성 물질을 렌즈 내부와 표면 전체에 화학적으로 균일하게 분포시키기에 적절한 방법이며, IPN 방법은 상분리 없이 렌즈 표면에 기능성을 부여하기에 적절한 방법으로 여겨진다.

키워드

콘택트렌즈;화학적 공유결합;단백질 흡착;함수율;하이드로겔

참고문헌

  1. Takanori S, Tatsuro G, Norihiko M, Madoka T, Kazuhiko I. Super-hydrophilic silicone hydrogels with interpenetrating poly(2-methacryloyloxyethyl phosphorylcholine) networks. Science Direct Biomaterials. 2010;31:3274-3280.
  2. Doerte L, Lyndon J. Protein deposition on contact lenses: The past, the present, and the future. Contact Lens & Anterior Eye. 2012;35(2):53-64. https://doi.org/10.1016/j.clae.2011.12.005
  3. Garrett Q, Laycock B, Garrett RW. Hydrogel lens monomer constituents modulate protein sorption. Ophthalmology & Visual Science. 2000;41(7):1687-1695.
  4. Andrea W, Doerte L, Adrienne B, Lyndon J, Heather S. Hyaluronic acid as an internal wetting agent on model DMAA/TRIS contact lenses. Journal of Biomaterials applications. 2011;27(4):423-432.
  5. Sung YJ, Ryu GC, Jun J. Adsorption properties of the lysozyme and albumin with physicochemical properties of the contact lens. J Korean Ophthalmic Opt. Soc. 2013; 18(3):261-270. https://doi.org/10.14479/jkoos.2013.18.3.261
  6. Lord MS, Stenzel MH, Simmons A, Milthorpe BK. Lysozyme interaction with poly(HEMA)-based hydrogel. Biomaterials. 2006;27(8):1341-1345. https://doi.org/10.1016/j.biomaterials.2005.09.007
  7. Prager MD, Quintana RP. Radiochemical studies on contact lens soilation. I. Lens uptake of 14C-lysozyme from simple and complex artificial tear solutions. J Biomed Mater Res. 1997;36(1):119-124. https://doi.org/10.1002/(SICI)1097-4636(199707)36:1<119::AID-JBM14>3.0.CO;2-D
  8. Sack RA, Jones B, Antignani A, Libow R, Harvey H. Specificity and biological activity of the protein deposited on the hydrogel surface. Invest Ophthalmol Visual Science. 1987;28(5):842-849.
  9. Hume E, Sack R, Stapleton F, Willcox M. Induction of cytokines from polymorphononuclear leukocytes and epithelial cells by ocular isolates of Serratia Marcescens. Ocular Immunol Inflammation. 2004;12(4):287-295. https://doi.org/10.1080/092739490500318
  10. Bengani LC, Leclerc J, Chauhan A. Lysozyme transport in p-HEMA hydrogel contact lenses, J Colloid Interface Sci. 2012;386(1):441-450. https://doi.org/10.1016/j.jcis.2012.07.018
  11. Rah MJ. A review of hyaluronan and its ophtjalmic applications. American Optometric Association. 2011;82:38-43. https://doi.org/10.1016/j.optm.2010.08.003
  12. Kim HJ, Kim SH, Kim JM. A study on improvement of wettability and comfort in contact lens with hyaluronic acid. J Korean Ophthalmic Opt Soc. 2011;16(3):255-264.
  13. Mark VB, Lyndon J, Heather S. Hyaluronic acid containing hydrogels for the reduction of protein adsorption. Science Direct Biomaterials. 2008;29(7):780-789.
  14. Scheuer CA, Fridman KM, Barniak VL, Burke SE, Venkatesh S, Venkatesh S. Retention of conditioning agent hyaluronan on hydrogel contact lenses. Contact Lens & Anterior Eye. 2010;33S:S2-S6.
  15. Zhenyu F, Chunlian H, Hebing L, Chong Y, Limiao C, Jianhan H, et al. A novel hydrophilic-hydrophobic magnetic interpenetrating polymer networks(IPNs) and its adsorption towards salicylic acid from aqueous solution. Chemical Engineering Journal. 2015;279:250-257. https://doi.org/10.1016/j.cej.2015.04.146
  16. Jeong KS, Kim HJ, Lim HL, Ryu GC, Seo ES, You NH, et al. Synthesis and biocompatibility of silicone hydrogel functionalized with polysaccharide. Bulletin of the Korean Chemical Society. 2015;36(6):1649-1653. https://doi.org/10.1002/bkcs.10315
  17. Choi JY, Park JS, Kim SR, Park MJ. The change in refractive powers of soft contact lenses caused by the deposition of tear proteins. J Korean Ophthalmic Opt Soc. 2011;16(4): 383-390.
  18. Keith D, Hong B, Christensen M. A novel procedure for the extraction of protein deposits from soft hydrophilic contact lenses for analysis. Current Eye Research. 1997; 16(5):503-510. https://doi.org/10.1076/ceyr.16.5.503.7049
  19. Cho NR, Ryu GC, Jun J. The properties of hyaluronan addition on the protein adsorption at the silicone hydrogel contact lens. Korean J Vis Sci. 2014;16(1):99-109.
  20. van Beek M, Weeks A, Jones L, Sheardown H. Immobilized hyaluronic acid containing model silicone hydrogels reduce protein adsorption. J Biomater Sci Polym Ed. 2008;19(11):533-537.
  21. Norde W, Lyklema J. Why proteins preferinterfaces. J Biomater Sci Polym Ed. 1991;2(3):183-202. https://doi.org/10.1080/09205063.1991.9756659