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Inhibitory effect of Angelica gigas extract powder on induced inflammatory cytokines in rats osteoarthritis

참당귀 추출분말의 골관절염 흰쥐의 염증성 사이토카인류의 억제활성

  • Kwon, Jin-Hwan (Natural Products Medical Research Institute, Nutragen Co.) ;
  • Han, Min-Seok (Natural Products Medical Research Institute, Nutragen Co.) ;
  • Lee, Bu-Min (College of Pharmacy, Chungbuk National University) ;
  • Lee, Yong-Moon (College of Pharmacy, Chungbuk National University)
  • 권진환 (뉴트라젠 천연물의학연구소) ;
  • 한민석 (뉴트라젠 천연물의학연구소) ;
  • 이부민 (충북대학교 약학대학 생물분석화학교실) ;
  • 이용문 (충북대학교 약학대학 생물분석화학교실)
  • Received : 2015.07.07
  • Accepted : 2015.08.08
  • Published : 2015.08.25

Abstract

The protective effects of extract powder of Angelica gigas on the degeneration of the articular cartilage in rats was investigated with monosodium iodoacetate (MIA)-induced osteoarthritis, The treatment of high concentration (50 μg/mL) of Angelica gigas effectively inhibited nitric oxide (NO) production induced by interleukin-1α (IL-1α) without any cytotoxicity. Specifically, mRNA and protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were dose dependently reduced by extract powder of Angelica gigas. Importantly, mRNA expression in articular cartilage of inflammatory cytokines, tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6) were clearly reduced. The inflammatory cytokines in blood were also reduced as well. These results suggested that the protective effects on the degeneration of the articular cartilage was derived from the inhibitory effects of mRNA and protein expression of tested inflammatory cytokines which is linked to prevent the degradation of proteoglycan (PG), the main matrix content in articular cartilage. Meanwhile, the 2 hrs incubation of decursin, a major compound of extract powder in rat whole blood rapidely converted decursin into decursinol which shows string anti-inflammatory activity. The coverted decursinol was detected after 8 hrs in whole blood by LC-MS/MS. Conclusively, the inhibitory effects of inflammatory cytokines production in osteoarthritis may be derived from the production of decursinol, which performs against inflammatroy cytokines like TNF-α, IL-1β, and IL-6.

Keywords

Angelica gigas;osteoarthritis;inflammatory cytokines;$TNF-\alpha$;decursin;decursinol

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