A Reusable Pb2+ Detecting Aptasensor Employing a Gold Nanorod-DNAzyme Conjugate

  • Lee, Jayeon ;
  • Ha, Tai Hwan
  • Received : 2015.09.02
  • Accepted : 2015.09.16
  • Published : 2015.09.30


Here, we demonstrated a $Pb^{2+}$ detecting aptasensor using $Pb^{2+}$-sensitive DNAzyme-conjugated gold nanorods (GNRs). Fluorescent DNA substrates that were initially quenched by GNRs, are released in response to $Pb^{2+}$ ions to give a substantial fluorescence signal. The GNR-tethered DNAzyme is reusable at least three times with a LOD of 50 nM.


DNAzyme;Heavy metal ions;Gold nanorod;Aptamer


  1. Bellinger, D.C., K.M. Stiles, and H.L. Needleman, Low-Level Lead Exposure, Intelligence and Academic Achievement: A Long-term Follow-up Study. Pediatrics, 90, 855-861 (1992).
  2. Menke, A., et al., Blood Lead Below 0.48 ${\mu}mol/L$ (10 ${\mu}g/dL$) and Mortality Among US Adults. Circulation, 114, 1388-1394 (2006).
  3. Abadin H, A.A., Stevens YW, et al., Toxicological Profile for Lead. 2007 Aug. 3: Atlanta (GA): Agency for Toxic Substances and Disease Registry (US).
  4. Ma, R., W. Van Mol, and F. Adams, Determination of cadmium, copper and lead in environmental samples. An evaluation of flow injection on-line sorbent extraction for flame atomic absorption spectrometry. Analytica Chimica Acta, 285, 33-43 (1994).
  5. Ochsenkuhn-Petropoulou, M. and K.M. Ochsenkuhn, Comparison of inductively coupled plasma-atomic emission spectrometry, anodic stripping voltammetry and instrumental neutron-activation analysis for the determination of heavy metals in airborne particulate matter. Fresenius' Journal of Analytical Chemistry, 369, 629-632 (2001).
  6. Shen, H., et al., Stripping voltammetry of copper and lead using gold electrodes modified with self-assembled monolayers. Journal of Solid State Electrochemistry, 1, 241-247 (1997).
  7. Park, S., et al., Rhodamine hydrazone derivatives bearing thiophene group as fluorescent chemosensors for Hg2+. Dyes and Pigments, 99, 323-328 (2013).
  8. Li, J. and Y. Lu, A Highly Sensitive and Selective Catalytic DNA Biosensor for Lead Ions. Journal of the American Chemical Society, 122, 10466-10467 (2000).
  9. Kim, J.H., S.H. Han, and B.H. Chung, Improving Pb2+ detection using DNAzyme-based fluorescence sensors by pairing fluorescence donors with gold nanoparticles. Biosensors and Bioelectronics, 26, 2125-2129 (2011).
  10. Guo, Y., et al., A sensitive biosensor with a DNAzyme for lead(ii) detection based on fluorescence turn-on. Analyst, 140, 4642-4647 (2015).
  11. Miao, X., L. Ling, and X. Shuai, Ultrasensitive detection of lead(ii) with DNAzyme and gold nanoparticles probes by using a dynamic light scattering technique. Chemical Communications, 47, 4192-4194 (2011).
  12. Zhu, G. and C.-y. Zhang, Functional nucleic acidbased sensors for heavy metal ion assays. Analyst, 139, 6326-6342 (2014).
  13. Li, Z., et al., Multiple thiol-anchor capped DNA-gold nanoparticle conjugates. Nucleic Acids Research, 30, 1558-1562 (2002).
  14. Gole, A. and C.J. Murphy, Seed-Mediated Synthesis of Gold Nanorods: Role of the Size and Nature of the Seed. Chemistry of Materials, 16, 3633-3640 (2004).


Supported by : Korea Research Foundation