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A Reusable Pb2+ Detecting Aptasensor Employing a Gold Nanorod-DNAzyme Conjugate

  • Lee, Jayeon ;
  • Ha, Tai Hwan
  • Received : 2015.09.02
  • Accepted : 2015.09.16
  • Published : 2015.09.30

Abstract

Here, we demonstrated a $Pb^{2+}$ detecting aptasensor using $Pb^{2+}$-sensitive DNAzyme-conjugated gold nanorods (GNRs). Fluorescent DNA substrates that were initially quenched by GNRs, are released in response to $Pb^{2+}$ ions to give a substantial fluorescence signal. The GNR-tethered DNAzyme is reusable at least three times with a LOD of 50 nM.

Keywords

DNAzyme;Heavy metal ions;Gold nanorod;Aptamer

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Acknowledgement

Supported by : Korea Research Foundation