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Effects of Haedokgumhwa-san Water Extracts on LPS-induced Inflammatory Response in Macrophage

해독금화산 물추출물이 LPS로 유도된 대식세포의 염증반응에 미치는 영향

LIM, Jae-Soo;KANG, Ok-Hwa;SEO, Yun-Soo;KWON, Dong-Yeul
임재수;강옥화;서윤수;권동렬

  • Received : 2015.08.26
  • Accepted : 2015.10.02
  • Published : 2015.09.30

Abstract

Objectives : TheHaedokgumhwa-sanwater extract (HDKHS) is used in Korea, Japan and China as a traditional therapeutic agent to cure an infectious disease. But its study is not enough. Therefore, the present study focused on the elucidation of HDKHS to investigate the anti-inflammatory effects and to established the possible mechanisms involved in its action on LPS-stimulated immune response in murine macrophages.Methods : Inflammatory status was induced by LPS and measured by increasement of inflammatory mediators. LPS induced secretions of NO and PGE2in RAW 264.7 cells were measured using griess reagent and enzyme-linked immunosorbent assay (ELISA) kit respectively. production of IL-6 was examined using ELISA kit and expression of IL-6 mRNA was measured by RT-PCR method. To investigate the effects of HDKHS on inflammatory mediators, such as iNOS, COX-2 and MAPKs, western blot and RT-PCR were performed.Results : HDKHS significantly reduced production of NO and PGE2 which were induced by LPS. Also, activation of IL-6 was reduced both protein and mRNA levels. The expressions of inflammatory mediator include iNOS and COX-2 were decreased by pretreatment with HDKHS. futhermore The result showed HDKHS down-regulate the LPS induced phosphorylation of ERK 1/2, one of the MAPK family, which is considered as a main regulator of transmission from pathogens to nucleus of immune cells.Conclusions : Our results suggest that the anti-inflammatory properties of HDKHS may stem from the inhibition of pro-inflammatory mediators via suppression of initiation of inflammatory response by inhibiting MAPKs signaling pathways.

Keywords

Haedokgumhwa-san;Anti-inflammatory activity;Macrophages;Mitogen-activated protein kinases (MAPKs)

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Acknowledgement

Grant : 한방체액조절연구센터