Antioxidant capacity and nutritive components from biji sub-micron suspension by ultra-high pressure homogenization process

초고압균질 가공에 의해 제조된 초미세 비지 현탁액의 영양성분 및 항산화성

  • Lee, Seon-Ho (Department of Food Science and Technology, Yeungnam University)
  • 이선호 (영남대학교 식품공학과)
  • Received : 2015.09.14
  • Accepted : 2015.10.03
  • Published : 2015.10.30


Biji is a tofu by-product made by squeezing the liquid out of the dregs left over from making tofu. The objectives of this research were to break the biji into sub-micron-scale particles and to prepare a biji sub-micron suspension having increased quality characteristics such as antioxidant capacity, polyphenol, dietary fiber, and soluble protein contents, in comparison with untreated biji. Disruption of biji by an ultra-high-pressure homogenization (UHPH) process was used to prepare the sub-micron suspension of biji. UHPH can be used to prepare emulsions or suspensions with extremely small particle sizes. The effect of the UHPH process on biji was studied at 1,000, 1,500, and 2,000 bar. The extraction yield increased up to 1.64 times by breaking the biji to form a sub-micron suspension. The soluble dietary fiber, protein, and free amino acid contents were increased by the UHPH process (p<0.05), in comparison with those of untreated biji. Furthermore, the total sugar, free sugar, and total polyphenol contents of biji were augmented by the UHPH process. Antioxidant activity (ABTS) after exposure to UHPH (1,000-2,000 bar) was well retained (p<0.05). The extraction efficiencies and nutritive components were enhanced by increasing the pressure in the UHPH process.


  1. Woo EY, Kim MJ, Shin WS, Lee KA, Kim KS (2001) Production of protein hydrolyzate, that can be used as food additives, from Okara. Korean J Food Sci Technol, 33, 1769-773
  2. Van der Reit WB, Wight AW, Clillierers KR, Datel JM (1989) Food chemical investigation of Tofu and its by product Okara. Food Chem, 34, 193-202
  3. Hackler LR, Stillings BR, Ploimeni RJ Jr (1967) Correlation of amino acid indexes with nutritional quality of several soybean fraction. Cereal Chem, 44, 638-644
  4. Lee WJ, Choi MR, Sosulski FW (1992) Separation of tofu-residue (biji) into dietary fiber and protein fractions. Korean J Food Sci Technol, 24, 97-100
  5. Hackler LR, Hand DB, Steinkraus KH, Van Buren JP (1963) A comparison of the nutritional value of protein from several soybean fractions. J Nutr, 80, 205-210
  6. Chung SS, Chang HN, Park MY. (1978) Dehydration of soybean residue by hot-air in conjunction with filter pressing. Korean J Food Sci Technol, 10, 1-7
  7. Kim DS, Seol NH, Kim HD (1996) Change in quality of soybean curd residue as affected by different drying methods. J Korean Soc Food Nutr, 25, 453-459
  8. Song YC, Lee SP (2013) Evaluation in physicochemical properties of soy sauce fortified with soymilk residue (okara koji). Korean J Food Preserv, 20, 818-826
  9. Youn UK, Kim YH, Kim SD (2003) Pigment and monacolin K content of beni-koji fermented with soybean curd residue. Korean J Food Preserv, 10, 360-364
  10. Park DJ, Kim BK (2008) Food nanoparticles. Food Sci Industry, 41, 17-24
  11. Suarez-Jacobo a, Rufer CE, Gervilla R, Guamis B, Roig-Sagues AX, Saldo J (2011) Influence of ultra-high pressure homogenisation on antioxidant capacity, polyphenol and vitamin content of clear apple juice. Food Chem, 127, 447-454
  12. Huang X, Tu Z, Xiao H, Li Z, Zhang Q, Wang H, Hu Y, Zhang L (2013) Dynamic high pressure microfluidizationassisted extraction and antioxidant activities of sweet potato (Ipomoea batatas L.) leaves flavonoid. Food Bioprod Process, 91, 1-6
  13. Wu L, Pang GC, Chen QS (2008) Study on spirulina cells fragmentation process by high pressure homogenizer. J Food Sci, 3, 002
  14. Yu R (2012) Preparation of Chlorella vulgaris sub-micron suspension & it's impact on lutein extractability and suspension stability. MS Thesis. Chungang University, Korea
  15. Patras A, Brunton N, Da Pieve S, Butler F, Downey G (2009) Effect of thermal and high pressure processing on antioxidant activity and instrumental colour of tomato and carrot purees. Innovative Food Sci Emerging Tech, 10, 16-22
  16. An EM, Lee JK, Choi YS, Kim YH, Shin HS (2014) Physicochemical properties of wheat bran in different areas prepared by a high-pressure homogenizer process. Korean J Food Sci Technol, 46, 404-409
  17. AACC (2000) Approved methods of the AACC. 10th ed, American Association of Cereal Chemists, St. Paul, MN, USA
  18. AOAC (1990) Official methods of analysis. 15th ed, Association of Official Analytical Chemists, Washington DC, USA
  19. Prosky L, Asp N, Swizer TF, Devries J, Furda I (1988) Determination of insoluble and total dietary fiber on foods and food products, Interlaboratory study. J AOAC Int, 71, 1017-1023
  20. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Biochem, 28, 350
  21. Luchsinger WW, Cornesky RA (1962) Reducing power by the dinirosalicylic acid method. Anal Biochem, 4, 346-347
  22. Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem, 193, 265-275
  23. Folin O, Denis W (1912) On phosphotungsticphosphomolybdic compounds as color reagents. J Biol Chem, 12, 239-243
  24. Gutfiger T (1981) Polyphenolsoils. J Am Oil Chem Soc, 58, 966-968
  25. Blois MS (1958) Antioxidant determinations by the use of a stable free radical. Nature, 4617, 1199-1200
  26. Lee MS, Kim KH, Lee GJ (1987) Microbiological studies and biochemical changes in fermenting soybean curd residue during fermentation. Korean J Food Sci Technol, 19, 520-527
  27. Im SK, Yoo SM, Kim TY, Chun HK (2004) Quality characteristics of bijijang in different fermentation conditions. Korean J Food Sci Technol, 36, 448-455
  28. Jeong MH, Choi WY, Seo YC, Kang HY, Choi GP, Lee HY (2010) Anticancer activity of Acer mono wood extracted by ultra high pressure extraction process. Korean J Medi Crop Sci, 18, 157-167
  29. Zhang S, Zhu J, Wang C (2004) Novel high pressure extraction technology. Int J Pharm, 278, 471-474
  30. Chen RZ, Zhang SQ, Liu ZQ (2007) Application of ultrahigh pressure technology in extracting active component from Chinese materia medica. Chinese Tradit Herbal Drugs, 38, 1905
  31. Chou SY, Chien PL, Chau CF (2008) Particle size reduction effectively enhances the cholesterol-lowering activities of carrot insoluble fiber and cellulose. J Agr Food Chem, 56, 10994-10998
  32. Kim CH, Kwon MC, Syed AQ, Hwang B, Nam JH, Lee HY (2007) Toxicity reduction and improvement of anticancer activities from Rhodiola sachalinensis A. Bor by ultra high pressure extracts process. Korean J Medicinal Crop Sci, 15, 411-416
  33. Lee GJ, Choi SD (2008) Application of biological industry using high hydrostatic pressure (HHP) system. Korean J Biotechnol Bioeng, 23, 362-368

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