Anti-inflammatory effect of barley leaf ethanol extract in LPS-stimulated RAW264.7 macrophage

LPS로 자극한 RAW264.7 대식세포에서 보리순 에탄올 추출물의 항염증 효과

  • Kim, Mee-Kyung (Department of Cosmetic Science and Technology, Seowon University) ;
  • Kim, Dae-Yong (Department of Pharmaceutical Science and Engineering, Seowon University)
  • Received : 2015.08.13
  • Accepted : 2015.10.08
  • Published : 2015.10.30


This study investigated the anti-inflammatory activity of barley leaf extract in lipopolysaccharide (LPS)-stimulated RAW264.7 cells and hairless mice. Pre-treatment with barley leaf extract significantly inhibited the protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-II (COX-II) in a dose-dependent manner in LPS-stimulated RAW264.7 cells. Barley leaf extract also significantly inhibited the secretion of inflammatory cytokines, such as tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), interleukin-$1{\beta}$ (IL-$1{\beta}$), and interleukin-6 (IL-6). Moreover, phosphorylation of mitogen-activated protein kinases (MAPKs) and nuclear translocation of nuclear factor-kappa B (NF-${\kappa}B$) were strongly suppressed by barley leaf extract in LPS-stimulated cells. In hairless mice, barley extract significantly decreased the pathological phenotypes of contact dermatitis, such as erythema, edema, and scabs. These results indicate that barley leaf extract has an anti-inflammatory effect and therefore a possible role in the treatment of inflammatory diseases or in functional cosmetics.


  1. Jnawali HN, Lee EJ, Jeong KW, Shin A, Heo YS, Kim YM (2014) Anti-inflammatory activity of rhamnetin and a model of its binding to c-Jun NH2-terminal kinase 1 and p38 MAPK. J Nat Prod, 77, 258-263
  2. Yun KJ, Kim JY, Kim JB, Lee KW, Jeong SY, Park HJ, Jung HJ, Cho YW, Yun K, Lee KT (2008) Inhibitionof LPS-induced NO and PGE2 production by asiatic acid via NF-kappa B inacti-vation in RAW264.7 macrophages : possible involvement of the IKK and MAPK pathways. Int Immunopharmacol, 8, 431-441
  3. Yoon HJ, Moon ME, Park HS, Im SY, Kim YH (2007) Chitosan oligosaccharide (COS) inhibits LPS-induced inflammatory effects in RAW264.7 macrophagecells. Biochem Biophys Res Commun, 358, 954-959
  4. Li CC, Hsiang CY, Lo HY, Pai FT, Wu SL, Ho TY (2012) Genipin inhibits lipopolysaccharide-induced acute systemic inflammation in mice as evidencedby nuclear factor-kappa B bioluminescent imaging-guided transcriptomic analysis. Food Chem Toxicol, 5, 2978-2986
  5. Xie C, Kang J, Li Z, Schauss AG, Badger TM, Nagarajan S, Wu T, Wu X (2012) The acaiflavonoid velutin is a potent anti-inflammatory agent : blockade of LPS-mediated TNF-${\alpha}$ and IL-6 production through inhibiting NF-kappa B activation and MAPK pathway. J Nutr Biochem, 23, 1184-1191
  6. Kim MK, Lee IC (2014) Anti-oxidant and anti-inflammatory effects of extracts from blueberry (Vaccinium ashei) leaf. J Korean Soc Beauty Art, 15, 109-120
  7. Yoon MY, Han EH, Han YS (2013) A study on anti-oxidant activity and whitening action of Plantago asiatica L. seed extract. J Korean Soc Beauty Art, 14, 259-269
  8. You SH, Yoon MY (2013) Effects of anti-oxdiant activity and anti-inflammation action on extracts isolated from A. crenata frui. J Korean Soc Beauty Art, 14, 197-208
  9. Kim YR, Han JS (2014) A study of antimicrobial and anti-oxidative activities on skin inflammation-causing microbes of supercritical green tea extract. J Korean Soc Beauty Art, 14, 71-80
  10. Kim DC (2006) Preparation of barley leaf powder tea and its quality characteristics. Korean Soc Food Sci Nutr, 35, 734-737
  11. Berridge MV, Tan AS (1993) Characterization of the cellular reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) : subcellular localization, substrate dependence, and involvement of mitochondrial electron transport in MTT reduction. Arch Biochem Biophys, 303, 474-482
  12. Ghayor C, Gjoksi B, Siegenthaler B, Weber FE (2015) N-methyl pyrrolidone (NMP) inhibits lipopolysaccharideinduced inflammation by suppressing NF-${\kappa}$B signaling. Inflamm Res. 64, 527-36
  13. Kim JE, Lee YK, Nam SH, Choi SI, Goo JS, Jang MJ, Lee HS, Son HJ, Lee CY, Hwang DY (2010) The symptoms of atopic dermatitis in NC/Nga mice were significantly relieved by the water extract of Liriope platyphylla. Lab Anim Res 26, 377-384
  14. Li Y, Wu Q, Deng Y, Lv H, Qiu J, Chi G, Feng H (2015) D(-)-Salicin inhibits the LPS-induced inflammation in RAW264.7 cells and mouse models. Int Immunopharmacol 26, 286-94
  15. Deveraux QL, Roy N, Stennicke HR, Van Arsdale T, Zhou Q, Srinivasula SM, Alnemri ES, Salvesen GS, Reed JC (1998) IAPs block apoptotic events induced by caspase-8 and cytochrome c by direct inhibition of distinct caspases. Embo J, 17, 2215-2223
  16. An MS, Kim SH, Yoon HK, Kim WW (2009) COX-2 expression in malignant breast tumors. J Korean Surg Soc, 77, 371-377
  17. Jung JY, Lee JR, Byun SH, Jung JW, Kim YH, Kim SC (2010) Inhibitory effect of dioscorea bulbifera MeOH extract on pro-inflammatory mediator In vitro and In vivo. Korean J Oriental Physiol Pathol, 24, 310-318
  18. Choi JY, Lee SY, Cha JH (2009) Expression of iNOS and NADPH-diaphroase reactivity in the lipopolysaccharide treated rat kidney. Korean J Anat, 42, 265-275
  19. Kim DH, Hwang EY, Son JH (2013) Anti-inflammatory activity of Carthamus tinctorious seed extracts in RAW264.7 cell. J Life Sci, 23, 55-62
  20. Yang H, Oh KH, Yoo YC (2015) Anti-inflammatory effect of hot water extract of aronia fruits in LPS-stimulated RAW 264.7 macrophages. J Korean Soc Food Sci Nutr, 44, 7-13
  21. Park JS, Jung SH (2013) Effects of sandalwood essential oil on the iNOS expression and proinflammatory cytokine production. J Pharm Soc Korea, 57, 70-75
  22. Kim BH, Lee YT, Kang KH (2010) Codonopsis lanceolata inhibits inflammation through regulation of MAPK in LPS-stimulated RAW264.7 cells. Korean J Oriental Physiol Pathol, 24, 80-84
  23. Kim BW, Kim JI, Kim HR, Byun DS (2014) Antiinflammatory effect of an ethyl acetate fraction from myagropsis yendoi on lipopolysaccharides-stimulated RAW 264.7 Cells. Korean J Fish Aquat Sci, 47, 527-536
  24. Chung HJ, Lee HS, Shin JS, Lee SH, Park BM, Youn YS, Lee SK (2010) Modulation of acute and chronic inflammatory processes by a traditional medicine preparation GCSB-5 both in vitro and in vivo animal models. J Ethnopharmacol, 130, 450-459
  25. Kim MS, Pyun HB, Hwang JK (2014) Panduratin A, an activator of PPAR-${\alpha}$/${\delta}$, suppresses the development of oxazolone-induced atopic dermatitis-like symptoms in hairless mice. Life Sci, 100, 45-54
  26. Lu CL, Wei Z, Min W, Hu MM, Chen WL, Xu XJ, Lu CJ (2015) Polysaccharides from Smilax glabra inhibit the pro-inflammatory mediators via ERK1/2 and JNK pathways in LPS-induced RAW264.7 cells. Carbohydr Polym, 20, 428-436
  27. Karuppagounder V, Arumugam S, Thandavarayan RA, Pitchaimani V, Sreedhar R, Afrin R, Harima M, Suzuki H, Nomoto M, Miyashita S, Suzuki K, Watanabe K (2014) Resveratrol attenuates HMGB1 signaling and inflammation in house dust mite-induced atopic dermatitis in mice. Int Immunopharmacol, 23, 617-623
  28. Lee HS, Kim DH, Hong JE, Lee JY, Kim EJ (2015) Oxyresveratrol suppresses lipopolysaccharide-induced inflammatory responses in murine macrophages. Hum Exp Toxicol, 34, 808-818
  29. Kim MS, Jeong JS, Lee HY, Ju YS, Bae GS, Seo SW, Cho IJ, Park SJ, Song HJ (2011) The anti-inflammatory effect of Achyranthes japonica on lipopolysaccharideinduced inflammatory activity in murine macrophages. Korean J Herbology, 26, 51-57
  30. Kim YS, Lee SJ, Hwang JW, Kim EH, Park PJ, Jeong JH (2012) Anti-inflammatory effects of extracts from Ligustrum ovalifolium H. leaves on RAW264.7 Macrophages. J Korean Soc Food Sci Nutr, 41, 1205-1210
  31. Jeong DH, Kim KBWR, Kim MJ, Kang BK, Bark SW, Pak WM, Kim BR, Ahn NK, Choi YU, Ahn DH (2014) Anti-inflammatory effect of ethanol extract from Sargassum fulvellum on iipopolysaccharide induced inflammatory responses in RAW 264.7 cells and mice ears. J Korean Soc Food Sci Nutr, 43, 1158-1165
  32. Kim BH, Lee YT, Kang KH (2010) Codonopsis lanceolata inhibits inflammation through regulation of MAPK in LPS-stimulated RAW264.7 cells. Korean J Oriental Physiol Pathol, 24, 80-84
  33. Man MQ, Hatano Y, Lee SH, Man M, Chang S, Feingold KR, Leung DY, Holleran W, Uchida Y, Elias PM (2008) Characterization of a hapten-induced, murine model with multiple features of atopic dermatitis : structural, immunologic, and biochemical changes following single versus multiple oxazolone challenges. J Invest Dermatol, 128, 79-86
  34. Choi DI, Choi JY, Kim YJ, Lee JB, Kim SO, Shin HT, Lee SC (2015) Ethanol extract of peanut sprout exhibits a potent anti-inflammatory activity in both an oxazoloneinduced contact dermatitis mouse model and compound 48/80-treated HaCaT cells. Ann Dermatol, 27, 142-151
  35. Lee HJ, Jung M, Kim JH, Yoon NY, Choi EH (2012) The effect of adipose-derived stem cell-cultured media on oxazolone treated atopic dermatitis-like murine model. Ann Dermatol, 24, 181-188

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