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Anti-inflammatory effect of barley leaf ethanol extract in LPS-stimulated RAW264.7 macrophage

LPS로 자극한 RAW264.7 대식세포에서 보리순 에탄올 추출물의 항염증 효과

  • Kim, Mee-Kyung (Department of Cosmetic Science and Technology, Seowon University) ;
  • Kim, Dae-Yong (Department of Pharmaceutical Science and Engineering, Seowon University)
  • Received : 2015.08.13
  • Accepted : 2015.10.08
  • Published : 2015.10.30

Abstract

This study investigated the anti-inflammatory activity of barley leaf extract in lipopolysaccharide (LPS)-stimulated RAW264.7 cells and hairless mice. Pre-treatment with barley leaf extract significantly inhibited the protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-II (COX-II) in a dose-dependent manner in LPS-stimulated RAW264.7 cells. Barley leaf extract also significantly inhibited the secretion of inflammatory cytokines, such as tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), interleukin-$1{\beta}$ (IL-$1{\beta}$), and interleukin-6 (IL-6). Moreover, phosphorylation of mitogen-activated protein kinases (MAPKs) and nuclear translocation of nuclear factor-kappa B (NF-${\kappa}B$) were strongly suppressed by barley leaf extract in LPS-stimulated cells. In hairless mice, barley extract significantly decreased the pathological phenotypes of contact dermatitis, such as erythema, edema, and scabs. These results indicate that barley leaf extract has an anti-inflammatory effect and therefore a possible role in the treatment of inflammatory diseases or in functional cosmetics.

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