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Effect of hot water and ethanol extracts from Nelumbo nucifera Gaertner flower on lipid accumulation and reactive oxygen species (ROS) production in adipogenesis of 3T3-L1 cells

3T3-L1 세포분화 중 지방축적 및 ROS 생성에 대한 연화 열수 및 에탄올 추출물의 효과

  • Oh, Ji-Won (Department of Food Science and Biotechnology, Kangwon National University) ;
  • Lee, Jin-Ha (Department of Food Science and Biotechnology, Kangwon National University) ;
  • Lee, Ok-Hwan (Department of Food Science and Biotechnology, Kangwon National University) ;
  • Kim, Kye-Hoon (Department of Food Science and Technology, Kongju National University) ;
  • Kim, Hey-Ran (Department of Food Science and Technology, Kongju National University) ;
  • Lee, Hyo-Ku (Department of Food Science and Technology, Kongju National University)
  • 오지원 (강원대학교 식품생명공학과) ;
  • 이진하 (강원대학교 식품생명공학과) ;
  • 이옥환 (강원대학교 식품생명공학과) ;
  • 김계훈 (공주대학교 식품공학과) ;
  • 김혜란 (공주대학교 식품공학과) ;
  • 이효구 (공주대학교 식품공학과)
  • Received : 2015.09.08
  • Accepted : 2015.09.25
  • Published : 2015.10.30

Abstract

The present study was designed to investigate the effects of hot water and ethanol extracts of Nelumbo nucifera Gaertner flower on lipid accumulation and reactive oxygen species (ROS) production during adipogenesis in 3T3-L1 cells. 3T3-L1 preadipocytes were treated with both hot water and ethanol extracts for up to 8 days following standard induction of differentiation. Regarding anti-adipogenic activity, compared with the control, the hot water and ethanol extracts significantly inhibited lipid accumulation (37.4 and 66.6%, respectively) and ROS production (46.4 and 46.8%, respectively) during adipogenesis in 3T3-L1 cells. Treatment with hot water and ethanol extracts significantly inhibited mRNA expression of peroxisome proliferator-activated receptor gamma ($PPAR{\gamma}$) and CCAAT/enhancer-binding protein alpha ($C/EBP{\alpha}$), thereby reducing the mRNA expression of adipocyte-specific fatty acid binding protein (aP2). Moreover, both the extracts significantly inhibited mRNA expression of NADPH oxidase (NOX4). Overall, our research suggests that N. nucifera Gaertner flower extracts might be a valuable source of bioactive compounds that exhibit anti-adipogenic activity and could have applications in the field of medicine and food industry.

Acknowledgement

Supported by : 공주대학교

References

  1. Chang MS, Kim HM, Yang WM, Kim DR, Park EH, Ko EB, Choi MJ, Kim HY, Oh JH, Shim KJ, Yoon JW, Park SK (2007) Inhibitory effects of Nelumbo nucifera on tyrosinase activity and melanogenesis in Clone M-3 melanocyte cells. Korean J Herbol, 22, 87-94
  2. Kim MS, Park YJ, Sohn YW (2010) Antioxidative effect and melanogenesis of Nelumbo nucifera stamen extract on cultured Human skin melanoma cells injured by hydrogen peroxide. Korean J Plant Res, 23, 145-150
  3. Kwak WS, Lee SK, Lee KJ, Kim KH, Kim HR, Lee HK, Oh JW, Lee OH (2014) Nutritional components and antioxidant activities of Nelumbo nucifera Gaertner flower and its wine. Korean J Food Preserv, 21, 373-380 https://doi.org/10.11002/kjfp.2014.21.3.373
  4. Yoo JS (2014) Antioxidant and antiobesity effects of lotus (Nelumbo nucifera) root and seed grown in Korea. PhD Thesis. Inha University, Incheon, Korea, p 5-11
  5. Seo YM, Park YJ, Choi YS (2009) Study on the protective effect of Nelumbo nucifera GAERTN extract on cultured cerebral neuroglial cells damaged by hexavalent chromium. Flower Res J, 17, 242-245
  6. Oh YL (2010) Effect of flower extract on antioxidative of cultured C6 glioma cells damaged by glucose oxidase of reactive oxygen species in Nelumbo nucifera. J Korean Soc People Plants Environ, 13, 17-24
  7. Chang MS, Ko EB, Lee HJ, Kim JS, Kim JS, Jee SW, Kim HY, Yeom MH, Kim DH, Kim HK, Park SK (2011) The effects of Nelumbo nucifera on utraviolet-B irradiated human keratinocytes. Korean J Herbol, 26, 45-49
  8. Lee KS, Kim MG, Lee KY (2006) Antioxidative activity of ethanol extract from lotus (Nelumbo nucifera) leaf. J Korean Soc Food Sci Nutr, 35, 182-186 https://doi.org/10.3746/jkfn.2006.35.2.182
  9. Park SY, Hwang TY, Kim JH, Moon KD (2001) Quality changes of minimally processed lotus root (Nelumbo nucifera) with browning inhibitors. Korean J Postharvest Sci Technol, 8, 164-168
  10. Peng Q, Wei Z, Lau BH (1998) Fructus corni enhances endotherial cell antioxidant defenses. Gen Pharmacol, 31, 221-225 https://doi.org/10.1016/S0306-3623(97)00459-X
  11. Spiegelman BM, Flier JS (2001) Obesity and the regulation of energy balance. Cell, 104, 531-543 https://doi.org/10.1016/S0092-8674(01)00240-9
  12. Marcelin G, Chua S (2010) Contributions of adipocyte lipid metabolism to body fat content and implications for the treatment of obesity. Curr Opin Pharmacol, 10, 588-593 https://doi.org/10.1016/j.coph.2010.05.008
  13. Holst D, Grimaldi PA (2002) New factors in the regulation of adipose differentiation and metabolism. Curr Opin Lipidol, 13, 241-245 https://doi.org/10.1097/00041433-200206000-00002
  14. de Ferranti S, Mozaffarian D (2008) The perfect storm : obesity, adipocyte dysfunction, and metabolic consequences. Clin Chem, 54, 945-955 https://doi.org/10.1373/clinchem.2007.100156
  15. Attie AD, Scherer PE (2009) Adipocyte metabolism and obesity. J Lipid Res, 50, 395-359 https://doi.org/10.1194/jlr.R800057-JLR200
  16. Rosen ED, MacDougald OA (2006) Adipocyte differentiation from the inside out. Nat Rev Mol Cell Biol, 7, 885-896 https://doi.org/10.1038/nrm2066
  17. Kim KH (2009) Perspective in regulation of adipogenesis by bioactive food components. Food Sci Ind, 42, 51-57
  18. Lee OH, Kwon YI, Hong HD, Park CS, Lee BY, Kim YC (2009) Production of reactive oxygen species and changes in antioxidant enzyme activites during differentiation of 3T3-L1 adipocyte. J Korean Soc Appl Biol Chem, 52, 70-75
  19. Valko M, Moncol DJ, Cronin TD, Mazura M, Telser J (2007) Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol, 39, 44-84 https://doi.org/10.1016/j.biocel.2006.07.001
  20. Lee JY, Yu MR, An BJ (2010) Comparison of biological activity between Nelumbo nucifera G. extracts and comsmetics adding Nelumbo nucifera G.. J Life Sci, 20, 1241-1248 https://doi.org/10.5352/JLS.2010.20.8.1241
  21. Rosen ED (2005) The transcriptional basis of adipocyte development. Prostaglandins Leukot Essent Fatty Acid, 73, 31-34 https://doi.org/10.1016/j.plefa.2005.04.004
  22. Evans RM, Barish GD, Wang YX (2004) PPARs and the complex journey to obesity. Nat Med, 10, 355-361 https://doi.org/10.1038/nm1025
  23. Pablo C, Daiana S, Soledad C, Virginia D, Lelia D, Juan CC, Liliana NG (2011) N-Acetylcysteine reduces markers of differentiation in 3T3-L1 adipocytes. Int J Mol Sci, 12, 6936-6951 https://doi.org/10.3390/ijms12106936
  24. Sampson N, Koziel R, Zenzmaier C, Bubendorf L, Plas E, Jansen-Durr P, Berger P (2011) ROS signaling by NOX4 drives fibroblast-to-myofibroblast differentiation in the diseased prostatic stroma. Mol Endocrinol, 25, 503-515 https://doi.org/10.1210/me.2010-0340
  25. Basuroy S, Tcheranova D, Bhattacharya S, Leffler CW, Parfenova H (2011) Nox4 NADPH oxidase-derived reactive oxygen species, via endogenous carbon monoxide, promote survival of brain endothelial cells during TNF-${\alpha}$-induced apoptosis. Am J Physiol Cell Physiol, 300, C256-265 https://doi.org/10.1152/ajpcell.00272.2010
  26. Chance B, Oshino N (1971) Kinetics and mechanism of catalase in peroxisomes of the mitochondrial fraction. Biochem J, 112, 225-233
  27. Yang RZ, Wei XL, Gao FF, Wang LS, Zhang HJ, Xu YJ, Li CH, Ge YX, Zhang JJ, Zhang J (2009) Simultaneous analysis of anthocyanins and flavonols in petals of lotus (Nelumbo) cultivars by high-performance liquid chromatography-photodiode array detection/electrospray ionization mass spectrometry. J Chromatogr A, 1216, 106-112 https://doi.org/10.1016/j.chroma.2008.11.046
  28. Lee JJ, Park SY, Lee MY (2006) Effect of lotus root (Nelumbo nucifera G.) on lipid metabolism in rats with diet-induced hypercholesterolemia. Korean J Food Preserv, 13, 634-642
  29. Furukawa S, Fujita T, Shimabukuro M, Iwaki M, Yamada Y, Nakajima Y, Nakayama O, Makishima M, Matsuda M, Shimomura I (2004) Increased oxidative stress in obesity and its impact on metabolic syndrome. J Clin Invest, 114, 1752-1761 https://doi.org/10.1172/JCI21625