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CANON 공정에서 운전조건에 따른 질소 제거효율 및 미생물군집 변화

Variation of Nitrogen Removal Efficiency and Microbial Communities Depending on Operating Conditions of a CANON Process

  • 조경민 (부산대학교 사회환경시스템공학부) ;
  • 박영현 (부산대학교 사회환경시스템공학부) ;
  • 조순자 (부산대학교 미생물학과) ;
  • 이태호 (부산대학교 사회환경시스템공학부)
  • Jo, Kyungmin (Department of civil and environmental engineering, Pusan National University) ;
  • Park, Younghyun (Department of civil and environmental engineering, Pusan National University) ;
  • Cho, Sunja (Department of microbiology, Pusan National University) ;
  • Lee, Taeho (Department of civil and environmental engineering, Pusan National University)
  • 투고 : 2015.04.03
  • 심사 : 2015.06.20
  • 발행 : 2015.06.30

초록

질소화합물은 부영양화 등 수질을 악화시키는 결과를 초래하므로 질소 제거는 수처리에 있어 가장 중요한 문제들 중 하나이다. 본 연구에서는 독립영양탈질 공정인 CANON (Completely Autotrophic Nitrogen-removal Over Nitrite)을 이용하여 암모니아성 질소 제거 효율을 평가하고, 미생물 군집 분석을 수행하였다. AOB (Ammonium Oxidizing Bacteria)와 ANAMMOX(ANaerobic AMMonium OXidation)균을 동시에 식종하고, $37^{\circ}C$에서 유입 암모니아성 질소농도 100 mg-N/L와 아질산성 질소 농도 100 mg-N/L 조건으로 운전한 결과, 성공적인 CANON 반응이 유도되었다. 유입수에서 아질산성 질소를 제외시키고 암모니아성 질소(100 mg-N/L)만을 공급하였을 때, DO농도 0.4 mg/L 이상에서는 CANON의 성능이 악화되었지만, DO농도를 0.3 mg/L으로 낮추자 71.3%의 총 질소제거효율을 나타내었다. 유입 암모니아성 질소 농도를 50 mg-N/L로 낮추었을 때, 질소 제거효율이 급격히 악화되었다. 그러나 유입농도를 다시 100 mg-N/L로 증가시키자 14일 만에 이전의 질소제거성능을 회복하였고, 이후 $76.1{\pm}4.9%$의 총 질소제거효율을 나타냈다. 온도를 상온($20{\pm}1^{\circ}C$) 조건으로 전환하자 초기에는 불안정한 CANON 반응이 일어났지만, 23일 이후에는 안정적인 총 질소제거효율($70.0{\pm}2.6$%)을 유지하였다. PCR-DGGE를 이용한 미생물군집 분석 결과, 식종원과 CANON의 미생물군집은 확연한 차이를 나타냈지만, CANON의 각 조건에 따른 미생물군집은 크게 다르지 않았다. 따라서 질소제거 성능의 악화는 미생물군집을 구성하는 미생물종의 변화에 기인하기 보다는 구성 미생물종들의 질소제거 활성의 저하에 기인하는 것으로 생각된다. 이러한 결과는 AOB와 ANAMMOX균을 식종하여 CANON 반응을 성공적으로 유도한다면, 이후 농도나 온도의 변화에도 안정적인 미생물군집을 유지할 수 있다는 것을 의미한다.

키워드

캐논;아나목스;부분 질산화;미생물군집

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피인용 문헌

  1. Nitrogen removal characteristics of pigment wastewater using PAC-A/O process vol.32, pp.1, 2018, https://doi.org/10.11001/jksww.2018.32.1.019