Anticancer Effects of Curcuma C20-Dialdehyde against Colon and Cervical Cancer Cell Lines

  • Chaithongyot, Supattra (Department of Biochemistry, Faculty of Science, Khon Kaen University) ;
  • Asgar, Ali (Program in Biological Science, Faculty of Science, Khon Kaen University) ;
  • Senawong, Gulsiri (Department of Biochemistry, Faculty of Science, Khon Kaen University) ;
  • Yowapuy, Anongnat (Department of Biochemistry, Faculty of Science, Khon Kaen University) ;
  • Lattmann, Eric (Division of Pharmacy, School of Life and Health Sciences, Aston University) ;
  • Sattayasai, Nison (Department of Biochemistry, Faculty of Science, Khon Kaen University) ;
  • Senawong, Thanaset (Department of Biochemistry, Faculty of Science, Khon Kaen University)
  • Published : 2015.10.06


Background: Recent attention on chemotherapeutic intervention against cancer has been focused on discovering and developing phytochemicals as anticancer agents with improved efficacy, low drug resistance and toxicity, low cost and limited adverse side effects. In this study, we investigated the effects of Curcuma C20-dialdehyde on growth, apoptosis and cell cycle arrest in colon and cervical cancer cell lines. Materials and Methods: Antiproliferative, apoptosis induction, and cell cycle arrest activities of Curcuma C20-dialdehyde were determined by WST cell proliferation assay, flow cytometric Alexa fluor 488-annexin V/propidium iodide (PI) staining and PI staining, respectively. Results: Curcuma C20 dialdehyde suppressed the proliferation of HCT116, HT29 and HeLa cells, with IC50 values of $65.4{\pm}1.74{\mu}g/ml$, $58.4{\pm}5.20{\mu}g/ml$ and $72.0{\pm}0.03{\mu}g/ml$, respectively, with 72 h exposure. Flow cytometric analysis revealed that percentages of early apoptotic cells increased in a dose-dependent manner upon exposure to Curcuma C20-dialdehyde. Furthermore, exposure to lower concentrations of this compound significantly induced cell cycle arrest at G1 phase for both HCT116 and HT29 cells, while higher concentrations increased sub-G1 populations. However, the concentrations used in this study could not induce cell cycle arrest but rather induced apoptotic cell death in HeLa cells. Conclusions: Our findings suggest that the phytochemical Curcuma C20-dialdehyde may be a potential antineoplastic agent for colon and cervical cancer chemotherapy and/or chemoprevention. Further studies are needed to characterize the drug target or mode of action of the Curcuma C20-dialdehyde as an anticancer agent.


Antiproliferative activity;apoptosis;Curcuma C20-dialdehyde;cell cycle arrest;cervical;colon cancer


Supported by : National Research Council of Thailand


  1. Aggarwal BB, Kumar A, Bharti AC (2003). Anticancer potential of curcumin: preclinical and clinical studies. Anticancer Res, 23, 363-98.
  2. Aggarwal BB, Shishodia A (2006). Molecular targets of dietary agents for prevention and therapy of cancer. Biochem Pharmacol, 71, 1397-421.
  3. Ferlay J, Shin HR, Bray F, et al (2010). Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008. Int J Cancer, 127, 2893-917
  4. Guo LD, Chen XJ, Hu YH, et al (2013). Curcumin inhibits proliferation and induces apoptosis of human colorectal cancer cells by activating the mitochondria apoptotic pathway. Phytother Res, 27, 422-30.
  5. Guo SB, Tian Y, Jian LY (2013). Study on extraction process of Curcuma zedoaria volatile oil and its effect on anticancer. Asian J Chem, 25, 7617-20.
  6. Jemal A, Bray F, Center MM, et al (2011). Global Cancer Statistics. CA Cancer J Clin, 61, 69-90.
  7. Lattmann E, Sattayasai J, Sattayasai N, et al (2010). In-vitro and in-vivo antivenin activity of 2-[2-(5,5,8a-trimethyl-2-methylene-decahydro-naphthalen-1-yl)-ethylidene]-succinaldehyde against Ophiophagus hannah venom. J Pharm Pharmacol, 62, 257-62.
  8. Lee DS, Lee MK, Kim JH (2009). Curcumin induces cell cycle arrest and apoptosis in human osteosarcoma (HOS) cells. Anticancer Res, 29, 5039-44.
  9. Liu RH (2004). Potential synergy of phytochemicals in cancer prevention: mechanism of action. J Nutr, 134, 3479-85.
  10. Liu TY, Tan ZJ, Jiang L (2013). Curcumin induces apoptosis in gallbladder carcinoma cell line GBC-SD cells. Cancer Cell International, 13, 64.
  11. Milacic V, Banerjee S, Landis-Piwowar KR, et al (2008). Curcumin inhibits the proteasome activity in human colon cancer cells in vitro and in vivo. Cancer Res, 68, 7283-92.
  12. Mors WB, do Nascimento MC, Pereira BMR, et al (2000). Plant natural products active against snake bitethe molecular approach. Phytochemistry, 55, 627-42.
  13. O'Sullivan-Coyne G, O'Sullivan GC, O'Donovan TR, Piwocka K, McKenna SL (2009). Curcumin induces apoptosisindependent death in oesophageal cancer cells. Br J Cancer, 101, 1585-95.
  14. Pan MH, Ho CT (2008). Chemopreventive effects of natural dietary compounds on cancer development. Chem Soc Rev, 37, 2558-74.
  15. Peczuh MW, Hamilton AD (2000). Peptide and Protein Recognition by Designed Molecules. Chem Rev, 100, 2479-94.
  16. Prakobwong S, Khoontawad J, Yongvanit P, et al (2011). Curcumin decreases cholangiocarcinogenesis in hamsters by suppressing inflammation-mediated molecular events related to multistep carcinogenesis. Int J Cancer, 129, 88-100.
  17. Ramachandran C, Fonseca HB, Jhabvala P, Escalon EA, Melnick SJ (2002). Curcumin inhibits telomerase activity through human telomerase reverse transcritpase in MCF-7 breast cancer cell line. Cancer Lett, 184, 1-6.
  18. Saha SK, Khuda-Bukhsh AR (2013). Molecular approaches towards development of purified natural products and their structurally known derivatives as efficient anticancer drugs: Current trends. Eur J Pharmcol, 714, 239-48.
  19. Sasikumar B (2005). Genetic resources of Curcuma: diversity, characterization and utilization. Plant Genet Resour, 3, 230-51.
  20. Somasundaram S, Edmund NA, Moore DT, et al (2002). Dietary curcumin inhibits chemotherapy-induced apoptosis in models of human breast cancer. Cancer Res, 62, 3868-75.
  21. World Health Organization (2008). The Global Burden of Disease: 2004 Update. Geneva: World Health Organization.
  22. Xiao Y, Yang FQ, Li SP, et al (2008). Essential oil of Curcuma wenyujin induces apoptosis in huma hepatoma cells. World J Gastroenterol, 14, 4309-18.

Cited by

  1. SH003-induced G1 phase cell cycle arrest induces apoptosis in HeLa cervical cancer cells vol.16, pp.6, 2017,