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Anti-adipogenic Effects of the Water Extracts of Defatted Green Tea Seed Cake

녹차씨 압착박 열수 추출물의 지방세포 분화 억제 효과

  • Received : 2015.05.20
  • Accepted : 2015.06.24
  • Published : 2015.08.31

Abstract

The effect of the hot water extract of defatted green tea seed cake (GTSE) on lipid metabolism and the underlying mechanisms of lipolysis in mature 3T3-L1 adipocytes were investigated. In this study, we found that the naringenin content of GTSE was 5.5 mg/g; however, catechins were not detected. The intracellular lipid droplets were stained with Oil Red O dye and quantified. Compared to the control, lipid accumulation was significantly decreased by 52%, and intracellular triglyceride (TG) level was reduced by 33% after treatment with GTSE at a concentration of $40{\mu}g/mL$. To determine the mechanism of reduction in TG content, we determined the level of fatty acid synthase (FAS), phosphorylation of adenosine monophosphate-activated protein kinase (AMPK), and acetyl-coenzyme A carboxylase (ACC) in the cell model. Incubation of the 3T3-L1 adipocytes with GTSE stimulated AMPK and ACC phosphorylation in a dose-dependent manner, and decreased the expression of FAS.

Keywords

Defatted green tea seed cake;naringenin;3T3-L1 adipocyte;lipolysis

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Acknowledgement

Supported by : 농림수산식품기술기획평가원