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Inter simple sequence repeat (ISSR)-PCR based polymorphism of Agaricus bisporus strains and monokayon isolates

Inter simple sequence repeat (ISSR)-PCR에 의한 양송이버섯(Agaricus bisporus) 계통과 단핵균주의 다형성 분석

  • Min, Kyong-Jin (Graduate School of Future Convergence Technology, Hankyong National University) ;
  • Kong, Won-Sik (Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Kang, Hee-Wan (Graduate School of Future Convergence Technology, Hankyong National University)
  • 민경진 (한경대학교 미래융합기술대학원) ;
  • 공원식 (농촌진흥청 국립특작과학원 버섯과) ;
  • 강희완 (한경대학교 미래융합기술대학원)
  • Received : 2015.08.04
  • Accepted : 2015.09.30
  • Published : 2015.09.30

Abstract

Twenty Inter simple sequence repeat (ISSR) primers were used to assess genetic diversity of 64 Agaricus strains including 45 A. bisporus strains and other 19 Agaricus spp. ISSR primers, (GA)T, (AG)YC, (GA)C and (CTC) amplified PCR polymorphic bands between the Agaricus species or within A. bisporus strains. PCR polymorphic bands were inputted for UPGMA cluster analysis. The varieties, Saea, Saedo, Saejeong and Saeyeon that have recently been developed in Korea were involved in the same group with closely genetic relationship of coefficient similarity over 0.92, whereas, other Korean strains were genetically related to A. bisporus strains that were introduced from USA, Eroupe and Chinese. Furthermore, ISSR-PCR polymorphism could potentially be used to identify homokaryon isolates.

Keywords

Agaricus bisporus strains;Genetic diversity;ISSR;PCR polymorphism

Acknowledgement

Supported by : 농촌진흥청

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