Physiological activities of water extract and solvent fractions of Grifola frondosa

잎새버섯 물추출물 및 유기용매 분획물의 생리활성

  • Kim, Eun-Jung (Department of Biomedical Laboratory Science, Sangji University) ;
  • Kim, Jun-Ho (Department of Fine Chemistry and New Materials, Sangji University)
  • 김은정 (상지대학교 임상병리학과) ;
  • 김준호 (상지대학교 정밀화학신소재학과)
  • Received : 2015.08.11
  • Accepted : 2015.09.30
  • Published : 2015.09.30


This study was performed in order to analyze the fibrinolytic, thrombin inhibitory, anti-oxidative, acetylcholinesterase inhibitory, and immuno-enhancing activities of the water extract and solvent fractions isolated from Grifola frondosa. Fibrinolytic activity was analyzed using the fibrin plate method, and thrombin inhibitory activity was assayed using the substrate H-D-Phe-piparg- pna. Anti-oxidative activity was estimated using the DPPH assay, and AChE inhibitory activity was measured using the spectrophotometric method. Immuno-enhancing activity was examined using the nitric oxide (NO) production in RAW 264.7 macrophage cells. Cell viability was determined using the MTS assay. Fibrinolytic activities were the highest in water extract (1.55 plasmin units/mL) followed by water fraction (0.85 plasmin units/mL). The thrombin inhibitory activities of the water and ethyl acetate fractions were determined to be 76.43% and 72.59%, respectively. The acetylcholinesterase inhibitory activities of chloroform and hexane fractions exhibited values of 95.14% and 94.74%, respectively. The butanol fraction showed the highest anti-oxidative activity at 94.47%. Anti-proliferating activity against Raw 264.7 cells showed no cytotoxicity. The production of NO in Raw 264.7 cells increased up to 2-fold by adding the water fraction compared to the untreated control. These results suggest that Grifola frondosa may serve as a useful functional food for the enhancement of immune function and the prevention and therapy of cardiovascular diseases.


Anti-oxidative activity;Fibrinolytic activity;Grifola frondosa;Immuno-enhancing activity;Thrombin inhibitory activity


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