DOI QR코드

DOI QR Code

Characterization of a new commercial strain 'Mongdol' by intra-specific hyphal anastomosis in Pleurotus ostreatus

계통간 교잡에 의한 느타리 신품종 '몽돌'의 육성 및 그 특성

  • Oh, Min-Ji (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Kim, Eun-Jung (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Jung, Ji-Hoon (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Shin, Pyung-Gyun (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Kim, Eun-Sun (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Oh, Youn-Lee (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Jang, Kab-Yeul (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Kong, Won-Sik (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Yoo, Young-Bok (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA)
  • 오민지 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 김은정 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 정지훈 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 신평균 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 김은선 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 오연이 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 장갑열 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 공원식 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 유영복 (농촌진흥청 국립원예특작과학원 버섯과)
  • Received : 2015.09.04
  • Accepted : 2015.09.24
  • Published : 2015.09.30

Abstract

A new commercial strain "Mongdol" of oyster mushroom was developed by hyphal anastomosis. It was improved with hybridization between monokaryotic strain derived from Pleurotus ostreatus ASI 0627 and dikaryotic strain derived from P. ostreatus ASI 2929. The optimum temperature of mycelial growth and fruiting body development were $25{\sim}30^{\circ}C$ and $12{\sim}18^{\circ}C$, respectively. When two different media including PDA (potato dextrose agar medium) and MCM (mushroom complete medium) were compared, the mycelial growth of this mushroom was faster in MCM than in PDA. Similar result was observed with the control strain P. ostreatus ASI 2504. Analysis of the genetic characteristics of the new cultivar "Mongdol" showed a different DNA profile as that of the control strain ASI 2504, when RAPD (Random Amplified Polymorphic DNA) primer URP3 and URP6 were used. Fruiting body production per bottle was about 106 g using demonstration farms. The color of pileus was blackish gray and the stipe was long. Therefore, we expect that this new strain "Mongdol" will satisfy the consumer's demand for high quality mushrooms.

Keywords

Commercial strain 'Mongdol';Intra-specific hybrids;Oyster mushroom;Pleurotus ostreatus

Acknowledgement

Supported by : 국립원예특작과학원

References

  1. Baldrian P, Gabriel J, Pospisck M. 1999. Improved isolated of nucleic acids from basidiomycetes fungi. Biotechniques. 27:458-460.
  2. Bobek P, Ginter E, Juroviov M, Kuniak L, 1991. Cholesterol-Lowering Effect of the Mushroom Pleurotus ostreatus in Hereditary Hypercholesterolemic Rats. Annals of Nutrition & Metabolism 35:191-195 https://doi.org/10.1159/000177644
  3. Ministry of Agriculture, Food and Rural Affairs. 2014. 2013 The product record of crops for a special use. 18-19.
  4. Raper CA, Raper JR, Miller RE. 1972. Genetic analysis of the life cycle of Agaricus bisporus. Mycologia. 64:1088-1117. https://doi.org/10.2307/3758075
  5. Stamet P. 1993. Growing gourmet and medicinal mushroom. Ten Speed Press.
  6. Wasser SP, Weis AL, 1999. Medicinal properties of substances occurring in higher basidiomycete mushrooms: current perspectives(Review). International J. of Medicinal Mushrooms 1:31-62. https://doi.org/10.1615/IntJMedMushrooms.v1.i1.30
  7. Yoo Young-Bok, Kong Won-Sik, Oh Se-Jong, Cheong Jong-Chun, Jang Kab-Yeul, Jhune Chang-Sung. 2005. Trends of mushroom science and mushroom industry. Journal of Mushroom Science and Production 2:1-23.
  8. Yoo Young-Bok, Kim Eun-Jung, Kong Won-Sik, Jang Kab-Yeul, Shin Pyung-Gyun. 2012. Journal of Mushroom Science and Production 10(3):109-114.
  9. Yoo Young-Bok, Goo Chand-Duck, Kim Sung-Hwan, Seo Gun-Sik, Shin Hyun-Dong, Lee Jun-Woo, Lee Chang-Su, Jang Hyun-Yoo. 2010. Mushroom Science. Nature and People. Chapter 12:366-368.
  10. Zadrazil F. 1978. Cultivation of Pleurotus. The Biology and Cultivation of Edible Mushrooms. 521-557