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The effect of sterilization condition and UV-C irradiation on the reduction of contamination rate for oyster mushroom bottle culture

살균조건 및 UV-C 조사가 느타리버섯 병재배 오염율 감소에 미치는 영향

  • 백일선 (경기도농업기술원버섯연구소) ;
  • 지정현 (경기도농업기술원버섯연구소) ;
  • 정윤경 (경기도농업기술원버섯연구소) ;
  • 김정한 (경기도농업기술원버섯연구소) ;
  • 임재욱 (경기도농업기술원)
  • Received : 2015.09.09
  • Accepted : 2015.09.30
  • Published : 2015.09.30

Abstract

This study was conducted to reduce contamination ratio of oyster mushroom bottle cultivation. The optimal conditions of substrate sterilization for reducing of contamination ratio were at $121^{\circ}C$ for 90 min. In addition, UV-C irradiation is good for lower contamination ratio to continue over 6 hours at cooling and inoculation room after sterilization. The contamination ratio and density of microorganisms of substrate were showed 0% after sterilization at $121^{\circ}C$ for 90 min. Trichoderma sp., main pathogen of mushrooms, was detected from substrate after sterilized during 2 or 4 hours at $101^{\circ}C$ and $105^{\circ}C$, respectively. The amount of electricity used was the lowest at $121^{\circ}C$ for 90 min than that of other sterilization conditions. The UV-C irradiation treatment was used UV-C lamp(40 watts) in the inoculation room($56m^3$). The density of bacteria did not detected after UV-C irradiation for 6 hours. And the death ratio of bacteria and Trichoderma sp. was 99.9% after UV-C irradiation for 6 hours. However, in the same UV-C irradiation time, the death ration of Cladosporium sp. was 90.9%. Therefore, the death ratio of fungi was lower than that of bacteria at the same UV-C irradiation treatment.

Keywords

Contamination;Sterilization;Trichoderma sp.;UV-C irradiation

Acknowledgement

Supported by : 농촌진흥청

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