DOI QR코드

DOI QR Code

산겨릅나무 줄기 추출물의 세포독성 억제 및 라디칼 소거 활성

임태진
Rhim, Tae-Jin

  • 투고 : 2015.07.26
  • 심사 : 2015.08.28
  • 발행 : 2015.11.30

초록

The objective of this study was to investigate anticytotoxic and antioxidatative capacities of ethanol extracts from Acer tegmentosum Maxim (A. tegmentosum) stem in vitro. The extract at concentration of 200 ug/mL inhibited 10 and 20 ug/mL arsenic trioxide-induced cytotoxicity of HepG2 cells by 79.3 and 57.5%, respectively. The extract at concentration of 200 ug/mL inhibited 0.2 and 0.5 mM t-BHP-induced cytotoxicity of HepG2 cells by 66.3 and 35.7%, respectively. Antioxidative effects of the extract were examined via measurement of ABTS, superoxide, and peroxyl radical scavenging activities. ABTS radical scavenging activity of the extract was higher than that of ${\alpha}$-tocopherol. Superoxide scavenging activity of the extract was higher than that of catechin. Oxygen radical absorbance capacity of the extract was higher than that of ascorbic acid. Cupric reducing antioxidant capacity of the extract was higher than that of ${\alpha}$-tocopherol. The extract at concentrations of 100 and $500{\mu}g/mL$ inhibited 10 mM t-BHP-induced lipid peroxidation of HepG2 cells by 38.2 and 80.7%, respectively. The extract prevented supercoiled DNA strand breakage induced by hydroxyl or peroxyl radical. Total phenolic and flavonoid contents of the extract at concentration of $100{\mu}g/mL$ were 71.3 nmol/mL gallic acid and 18.8 nmol/mL catechin equivalents, respectively. Thus, strong cytoprotective and antioxidant effects of A. tegmentosum stem extract seem to be due to, at least in part, the prevention from free radicals-induced oxidation as well as high levels in polyphenolic contents.

키워드

Acer tegmentosum Maxim;Anticytotoxic;Radical scavenging

참고문헌

  1. Cha, Y., Park, D., Lee, C. H., Baek, S., Kim, S., Kim, J., Kim, J. H., 2006, Arsenic trioxide induces apoptosis in human colorectal adenocarcinoma HT-29 cells through ROS, Cancer Res. Treat., 38, 54-60. https://doi.org/10.4143/crt.2006.38.1.54
  2. Erel, O., 2004, A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation, Clin. Biochem., 37, 277-285. https://doi.org/10.1016/j.clinbiochem.2003.11.015
  3. Gao, S., Duan, X., Wang, X., Dong, D., Liu, D., Li, X., Sun, G., Li, B., 2013, Curcumin attenuates arsenicinduced hepatic injuries and oxidative stress in experimental mice through activation of Nrf2 pathway, promotion of arsenic methylation and urinary excretion, Food Chem. Toxicol., 59, 739-747. https://doi.org/10.1016/j.fct.2013.07.032
  4. Ahn, D. K., 1998, Illustrated Book of Korean Medicinal Herbs, Kyohak Publishing, Seoul, 523-524.
  5. Apak, R., Guclu, K., Ozyurek, M., Karademir, S. E., 2004, Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method, J. Agric. Food Chem., 52, 7970-7981. https://doi.org/10.1021/jf048741x
  6. Gebel, T. W., 1999, Arsenic and drinking water contamination, Science, 283, 1458-1459.
  7. Hiramoto, K., Ojima, N., Sako, K. I., Kikugawa, K., 1996, Effect of plant phenolics on the formation of the spin-adduct of hydroxyl radical and the DNA strand breaking by hydroxyl radical, Biol. Pharm. Bull., 19, 558-563. https://doi.org/10.1248/bpb.19.558
  8. Hong, B. K., Eom, S. H., Lee, C. O., Lee, J. W., Jeong, J. H., Kim, J. K., Cho, D. H., Yu, C. Y., Kwon, Y. S., Kim, M. J., 2007, Biological activities and bioactive compounds in the extract of Acer tegmen -tosum Maxim. stem, Korean J. Medicinal Crop Sci., 15, 296-303.
  9. Hu, C., Zhang, Y., Kitts, D. D., 2000, Evaluation of antioxidant and prooxidant activities of bamboo Phyllostachys nigra var. Henonis leaf extract in vitro, J. Agric. Food Chem., 48, 3170-3176. https://doi.org/10.1021/jf0001637
  10. Huang, D., Ou, B., Hampsch-Woodill, M., Flanagan, J. A., Prior, R. L., 2002, High-throughput assay of oxygen radical absorbance capacity (ORAC) using a multichannel liquid handling system coupled with a microplate fluorescence reader in 96-well format, J. Agric. Food Chem., 50, 4437-4444. https://doi.org/10.1021/jf0201529
  11. Kim, S., Hur, S. J., Kim, K. H., Gi, K. S., Whang, W. K., 2012, Antioxidant and anti-inflammatory compounds isolated from Acer tegmentosum, J. Medicinal Plants Res., 6, 3971-3976.
  12. Kim, S. H., Park, H. J., Choi, J. W., 2008, Hepatopro -tective activity of salidroside isolated from Acer tegmentosum Max on D-galactosamine induced hepatotoxicity in rats, Korean J. Orient. Physiol. Pathol., 22, 1525-1531.
  13. Knasmuller, S., Parzefall, W., Sanyal, R., Ecker, S., Schwab, C., Williamson, G., Hietsch, G., Langer, T., Darroudi, F., Natarajan, A. T., 1998, Use of metabolically competent human hepatoma cells for the detection of mutagens and anti-mutagens, Mutat. Res., 402, 185-202. https://doi.org/10.1016/S0027-5107(97)00297-2
  14. Kwon, H. N., Park, J. R., Jeon, J. R., 2008, Antioxidative and hepatoprotective effects of Acer tegmentosum M. extracts, J. Korean Soc. Food Sci. Nutr., 37, 1389-1394. https://doi.org/10.3746/jkfn.2008.37.11.1389
  15. Liu, F., Ooi, V. E. C., Chang, S. T., 1997, Free radical scavenging activities of mushroom polysaccharide extracts, Life Sci., 60, 763-771. https://doi.org/10.1016/S0024-3205(97)00004-0
  16. Liu, M., Li, X. Q., Weber, C., Lee, C. Y., Brown, J., Liu, R. H., 2002, Antioxidant and antiproliferative activities of raspberries, J. Agric. Food Chem., 50, 2926-2930. https://doi.org/10.1021/jf0111209
  17. Liu, Q., Shin, E., Ahn, M, Hwang, B. Y., Lee, M. K., 2011, Anti-adipogenic activity of Acer tegmentosum and its constituent, catechin in 3T3-L1 cells, Nat. Product Sci., 17, 212-215.
  18. Mosmann, T., 1983, Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays, J. Immunol. Methods, 65, 55-63. https://doi.org/10.1016/0022-1759(83)90303-4
  19. Selvaraj, Y., Yeager-Armstead, M., Murray, E., 2012, Protective and antioxidant role of selenium on arsenic trioxide-induced oxidative stress and genotoxicity in the fish hepatoma cell line PLHC-1, Environ. Toxicol. Chem., 31, 2861-2869. https://doi.org/10.1002/etc.2022
  20. Shim, K., Kim, D, Song, S., Qi, X., Yoon, Y., Kim, H., Lee, J., Oh, H., Kim, S., Lee, K., 2011, Hepatoprotection of different water extracts from Acer tegmentosum M. on CCl4-induced acute hepatotoxicity in mice: comparative efficacies between the extracts of boughs, twigs, and leaves, Mol. Cell. Toxicol., 7, 405-413. https://doi.org/10.1007/s13273-011-0051-8
  21. Shin, I., Sa, J., Shim, T., Lee, J., 2006, The physical and chemical properties and cytotoxic effects of Acer tegmentosum Maxim. extracts, J. Korean Soc. Appl. Biol. Chem., 49, 322-327.
  22. Singleton, V. L., Orthofer, R., Lamuela-Raventos, R. M., 1999, Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent, Methods Enzymol., 299, 152-178. https://doi.org/10.1016/S0076-6879(99)99017-1
  23. Sinha, M., Manna, P., Sil, P. C., 2007, Taurine, a conditionally essential amino acid, ameliorates arsenic-induced cytotoxicity in murine hepatocytes, Toxicol. in Vitro, 21, 1419-1428. https://doi.org/10.1016/j.tiv.2007.05.010
  24. Soria, E. A., Eynard, A. R., Bongiovanni, G. A., 2010, Cytoprotective effects of silymarin on epithelial cells against arsenic-induced apoptosis in contrast with quercetin cytotoxicity, Life Sci., 87, 309-315. https://doi.org/10.1016/j.lfs.2010.07.007
  25. Steel, R. G. D., Torrie, J. H., 1980, Principles and Procedures of Statistics, 2nd ed., McGraw-Hill, New York, 186-187.
  26. Tchounwou, P. B., Wilson, B. A., Abdelghani, A. A., Ishaque, A. B., Patlolla, A. K., 2002, Differential cytotoxicity and gene expression in human liver carcinoma (HepG2) cells exposed to arsenic trioxide, and monosodium acid methanearsonate (MSMA), Int. J. Mol. Sci., 3, 1117-1132. https://doi.org/10.3390/i3111117
  27. Tirmenstein, M. A., Nicholls-Grzemski, F. A., Zhang, J. G., Fariss, M. W., 2000, Glutathione depletion and the production of reactive oxygen species in isolated hepatocyte suspensions, Chem. Biol. Inter., 127, 201-217. https://doi.org/10.1016/S0009-2797(00)00180-0
  28. Tung, N. H., Ding, Y., Kim, S. K., Bae, K., Kim, Y. H., 2008, Total peroxyl radical-scavenging capacity of the chemical components from the stems of Acer tegmentosum Maxim, J. Agric. Food Chem., 56, 10510-10514. https://doi.org/10.1021/jf8020283
  29. Valko, M., Leibfritz, D., Moncol, J., Cronin, M. T. D., Mazur, M., Telser, J., 2007, Free radicals and antioxidants in normal physiological functions and human disease, Intl. J. Biochem. Cell. Biol., 39, 44-84. https://doi.org/10.1016/j.biocel.2006.07.001
  30. Vidyashankar, S., Mitra, S. K., Nandakumar, K. S., 2010, Liv.52 protects HepG2 cells from oxidative damage induced by tert-butyl hydroperoxide, Mol. Cell. Biochem., 333, 41-48. https://doi.org/10.1007/s11010-009-0202-6
  31. Wang, C. H., Hsiao, C. K, Chen, C. L., Hsu, L. I., Chiou, H. Y., Chen, S. Y., Hsueh, Y. M., Wu, M. M., Chen, C. J., 2007, A review of the epidemiologic literature on the role of environmental arsenic exposure and cardiovascular diseases, Toxicol. Appl. Pharmacol., 222, 315-326. https://doi.org/10.1016/j.taap.2006.12.022
  32. WHO, 2011, Guidelines for drinking-water quality, 4th ed., World Health Organization, Geneva, 315-318.
  33. Yu, T., Lee, J., Lee, Y. G., Byeon, S. E., Kim, M. H., Sohn, E., Lee, Y. J., Lee, S. G., Cho, J. Y., 2010, In vitro and in vivo anti-inflammatory effects of ethanol extract from Acer tegmentosum, J. Ethnopharmacol., 128, 139-147. https://doi.org/10.1016/j.jep.2009.12.042

과제정보

연구 과제 주관 기관 : 상지대학교