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염색체 배수화제를 이용한 4배체 수박품종 개발

오상아;민광현;최용수;박상빈;김영철;조송미
Oh, Sang A;Min, Kwang Hyun;Choi, Yong Soo;Park, Sang Bin;Kim, Young Cheol;Cho, Song Mi

  • 투고 : 2015.08.11
  • 심사 : 2015.10.23
  • 발행 : 2015.10.31

초록

고품질 수박을 생산하기 위하여 서로 다른 염색체 배수화제를 처리하여 3가지 4배체 육종라인(‘SA03-1’, ‘SA06-1’ and ‘SB01-1’)을 개발하였다. 최적의 4배체 유기조건들을 조사하기 위하여, 3가지 육종라인들의 4배체는 공변세포의 배수화된 엽록체 수를 조사하는 것으로 선발하였다. 4배체 유기율은 배수화제의 종류와 유전자형에 따라 다르게 나타났다. 가장 높은 4배체 유기율은 colchicine 1.0%에서 82.2%로 가장 높게 나타났다. 이들 잠재적인 4배체 라인들의 배수성은 flow cytometer와 염색체 수 분석을 이용하여 재확인하였다. 확보된 4배체 육종라인들의 절간은 그들 2배체와 비교하여 4배체와 유사하거나 짧게 나타난반면, 잎의 크기는 4배체에서 더크게 나타났다. 과중은 ‘SA03-1’, ‘SB01-1’ 라인에서는 다소 감소하였고, 과피 두께와 당도는 ‘SB01-1’라인에서만 2배체와 차이를 보여주었다. 4배체 육종라인은 수정능력을 가지고 있었고 종자는 열매마다 ‘SA03-1’ (21개), ‘SA06-1’ (62개), ‘SB01-1’ (34.7개)로 수확되었으며, 4배체 종자는 그들의 2배체보다 더 크고 두터웠다. 이러한 4배체 품종 육성 결과는 씨없는 수박의 신품종 육성을 위해 유용하게 활용될 것으로 기대된다.

키워드

Citrullus lanatus;Colchicine;2,6-dinitroaniline;Polyploidy;Seedless

참고문헌

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피인용 문헌

  1. Effect of Colchicine on Chromosome Doubling in Codonopsis lanceolata vol.29, pp.3, 2016, https://doi.org/10.7732/kjpr.2015.28.5.656
  2. Comparison of Yield and Growth Characteristics of Platycodon grandiflorum According to the Ploidy Levels and Growing Conditions vol.29, pp.3, 2016, https://doi.org/10.7732/kjpr.2015.28.5.656

과제정보

연구 과제번호 : 수박의 4배체 계통 생산기술 개발