1H-Nuclear Magnetic Resonance-Based Plasma Metabolic Profiling of Dairy Cows with Fatty Liver

  • Xu, Chuang (Department of Clinical Veterinary Medicine, Animal Science and Technology College, Heilongjiang August First Land Reclamation University) ;
  • Sun, Ling-wei (Jiangsu Engineering Technology Research Center of Meat Sheep & Goat Industry, Nanjing Agricultural University) ;
  • Xia, Cheng (Department of Clinical Veterinary Medicine, Animal Science and Technology College, Heilongjiang August First Land Reclamation University) ;
  • Zhang, Hong-you (Department of Clinical Veterinary Medicine, Animal Science and Technology College, Heilongjiang August First Land Reclamation University) ;
  • Zheng, Jia-san (Department of Clinical Veterinary Medicine, Animal Science and Technology College, Heilongjiang August First Land Reclamation University) ;
  • Wang, Jun-song (Center for Molecular Metabolism, School of Environmental and Biological Engineering, Nanjing University of Science Technology)
  • Received : 2015.05.18
  • Accepted : 2015.08.24
  • Published : 2016.02.01


Fatty liver is a common metabolic disorder of dairy cows during the transition period. Historically, the diagnosis of fatty liver has involved liver biopsy, biochemical or histological examination of liver specimens, and ultrasonographic imaging of the liver. However, more convenient and noninvasive methods would be beneficial for the diagnosis of fatty liver in dairy cows. The plasma metabolic profiles of dairy cows with fatty liver and normal (control) cows were investigated to identify new biomarkers using $^1H$ nuclear magnetic resonance. Compared with the control group, the primary differences in the fatty liver group included increases in ${\beta}$-hydroxybutyric acid, acetone, glycine, valine, trimethylamine-N-oxide, citrulline, and isobutyrate, and decreases in alanine, asparagine, glucose, ${\gamma}$-aminobutyric acid glycerol, and creatinine. This analysis revealed a global profile of endogenous metabolites, which may present potential biomarkers for the diagnosis of fatty liver in dairy cows.


Supported by : Chinese National Natural Science Foundation


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