DOI QR코드

DOI QR Code

Evaluation on Anti-fungal Activity and Synergy Effects of Essential Oil and Their Constituents from Abies holophylla

전나무 정유의 항진균 효과와 유효성분의 시너지효과 평가

Kim, Seon-Hong;Lee, Su-Yeon;Cho, Seong-Min;Hong, Chang-Young;Park, Mi-Jin;Choi, In-Gyu
김선홍;이수연;조성민;홍창영;박미진;최인규

  • Received : 2015.09.04
  • Accepted : 2015.10.19
  • Published : 2016.01.25

Abstract

This study was to investigate the antifungal activity of A. holophylla essential oil against dermatophytes, such as Epidermophyton floccosum, Trichophyton mentagrophytes and Trichophyton rubrum, and to determine the potential effective compound as dermatitis treatment. To evaluate the potential antifungal activities of A. holophylla essential oil and its fractions, paper disc diffusion and agar dilution method tested with morphological observation. Also, their major constituents were analyzed by GC/MS. To determine synergic effects of active ingredient from A. holophylla essential oil were carried out by checkerboard microtiter plate testing. The morphological changes of the dermatophytes exposed to active fraction G4 were observed by electron microscopes. As the results, the highest activities were identified in the fraction containing ${\alpha}$-bisabolol. A mixture of ${\alpha}$-bisabolol and bornyl acetate showed the synergy effects, expressing high potential effects. Also, morphological observation using electron microscopes showed a dramatic changes of cell membrane of E. floccosum and T. rubrum exposed to fraction G4 containing ${\alpha}$-bisabolol. In conclusion, A. holophylla essential oil and its constituents were expected to be used as antifungal agent or raw material for dermatitis therapy.

Keywords

Abies holophylla;dermatophytes;antifungal activity;essential oil;synergy effect

References

  1. Hammer, K.A., Carson, C., Riley, T. 1999. Antimicrobial activity of essential oils and other plant extracts. Journal of applied microbiology 86(6): 985-990. https://doi.org/10.1046/j.1365-2672.1999.00780.x
  2. Bakkali, F., Averbeck, S., Averbeck, D., Idaomar, M. 2008. Biological effects of essential oils-a review. Food and chemical toxicology 46(2): 446-475. https://doi.org/10.1016/j.fct.2007.09.106
  3. de Billerbeck, V.G., Roques, C.G., Bessiere, J.-M., Fonvieille, J.-L., Dargent, R. 2001. Effects of Cymbopogon nardus (L.) W. Watson essential oil on the growth and morphogenesis of Aspergillus niger. Canadian journal of microbiology 47(1): 9-17. https://doi.org/10.1139/cjm-47-1-9
  4. Janssen, A., Scheffer, J., Svendsen, A.B. 1987. Antimicrobial activities of essential oils. Pharmaceutisch Weekblad 9(4): 193-197. https://doi.org/10.1007/BF02029329
  5. Kalemba, D., Kunicka, A. 2003. Antibacterial and antifungal properties of essential oils. Current medicinal chemistry 10(10): 813-829. https://doi.org/10.2174/0929867033457719
  6. Kamatou, G.P., Viljoen, A.M. 2010. A review of the application and pharmacological properties of ${\alpha}$ -bisabolol and ${\alpha}$-bisabolol-rich oils. Journal of the American Oil Chemists' Society 87(1): 1-7. https://doi.org/10.1007/s11746-009-1483-3
  7. Kim, S.H., Lee, S.Y., Hong, C.Y., Jeong, H.S., Park, M.J., Choi, I.G. 2012. Antifungal activity of essential oil from Cryptomeria japonica against dermatophytic fungi. Journal of the Korean Wood Science and Technology 40(4): 276-286. https://doi.org/10.5658/WOOD.2012.40.4.276
  8. Kim, S.H., Lee, S.Y., Hong, C.Y., Jang, S.K., Lee, S.S., Park, M.J., Choi, I.G. 2013. Evaluation on Anti-Dermatophyte Effect of Larix (kaempferi) Essential Oil on the Morphological Changes of Eermatophyte Fungal Hyphae. Journal of the Korean Wood Science and Technology 41(3): 247-257. https://doi.org/10.5658/WOOD.2013.41.3.247
  9. Lee, J.H., Hong, S.K. 2009. Comparative analysis of chemical compositions and antimicrobial activities of essential oils from Abies holophylla and Abies koreana activities of essential oils from Abies holophylla and Abies koreana. Journal of microbiology and biotechnology 19(4): 372-377. https://doi.org/10.4014/jmb.0811.630
  10. Mirmirani, P., Hessol, N.A., Maurer, T.A., Berger, T.G., Nguyen, P., Khalsa, A., Gurtman, A., Micci, S., Young, M., Holman, S. 2001. Prevalence and predictors of skin disease in the Women's Interagency HIV Study (WIHS). Journal of the American Academy of Dermatology 44(5): 785-788. https://doi.org/10.1067/mjd.2001.112350
  11. Richardson, M., Peterson, J., Clark, A. 1992. Bioactivity screenings of plants selected on the basis of folkloric use or presence of lignans in a family. Phytotherapy Research 6(5): 274-278. https://doi.org/10.1002/ptr.2650060511
  12. Singh, R., Nath, G., Goel, R., Bhattacharya, S. 1998. Pharmacological actions of Abies pindrow Royle leaf. Indian journal of experimental biology 36(2): 187-191.
  13. Sjostrom, E. 1993. Wood chemistry. Elsevier Science.
  14. Skold, M., Karlberg, A.-T., Matura, M., Borje, A. 2006. The fragrance chemical ${\beta}$-caryophyllene-air oxidation and skin sensitization. Food and Chemical Toxicology 44(4): 538-545. https://doi.org/10.1016/j.fct.2005.08.028
  15. Woodfolk, J.A. 2005. Allergy and dermatophytes. Clinical microbiology reviews 18(1): 30-43. https://doi.org/10.1128/CMR.18.1.30-43.2005
  16. Yesilada, E., Honda, G., Sezik, E., Tabata, M., Fujita, T., Tanaka, T., Takeda, Y., Takaishi, Y. 1995. Traditional medicine in Turkey. V. Folk medicine in the inner Taurus Mountains. Journal of Ethnopharmacology 46(3): 133-152. https://doi.org/10.1016/0378-8741(95)01241-5

Cited by

  1. Antidermatophytic Activity of Ethanolic Extract fromCroton tiglium vol.2016, 2016, https://doi.org/10.1155/2016/3237586

Acknowledgement

Grant : 목재이용 기반기술 연구

Supported by : 국립산림과학원