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Biomodification of Ethanol Organolsolv Lignin by Abortiporus biennis and Its Structural Change by Addition of Reducing Agent

Abortiporus biennis에 의한 유기용매 리그닌의 생물학적 변환과 환원제 첨가에 따른 구조 변화

  • Received : 2015.09.14
  • Accepted : 2015.10.23
  • Published : 2016.01.25

Abstract

The main goal of this study was to investigate biomodification mechanism of lignin by white rot fungus, Abortiporus biennis, and to depolymerize ethanol organosolv lignin for industrial application. In nitrogen-limited culture, A. biennis polymerized mainly lignin showing a rapid increase of molecular weight and structural changes depending on incubation days. At the initial incubation days, cleavage of ether bonds increased phenolic OH content, while the results were contrary in of the later part of the culture. Based on these results, ascorbic acid as a reducing agent was used to induce depolymerization of lignin during cultivation with white rot fungus. As a result, the degree of increase of average molecular weight of lignin was significantly declined when compared with those of the ascorbic acid free-experiment, although the molecular weight of fungus treated sample slightly increased than that of control. Furthermore, lignin derived oligomers in culture medium were depolymerized with the addition of ascorbic acid, showing that the average molecular weight was 381 Da, and phenolic OH content was 38.63%. These depolymerized lignin oligomers were considered to be applicable for industrial utilization of lignin. In conclusion, A. biennis led to the polymerization of lignin during biomodification period. The addition of ascorbic acid had a positive effect on the depolymerization and increase of phenolic OH content of lignin oligomers in medium.

Keywords

biomodification;depolymerization;lignin oligomers;white rot fungus;Abortiporus biennis

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Cited by

  1. Degradation and polymerization of monolignols by Abortiporus biennis, and induction of its degradation with a reducing agent vol.54, pp.10, 2016, https://doi.org/10.1007/s12275-016-6158-9

Acknowledgement

Supported by : 한국연구재단