DOI QR코드

DOI QR Code

Characterization of Graphene Oxide Suspension for Fluorescence Quenching in DNA-Diagnostics

Kapitonov, A.N.;Alexandrov, G.N.;Vasileva, F.D.;Smagulova, S.A.;Timofeev, V.B.;Maksimova, N.R.;Kuznetsov, A.A.

  • Received : 2015.09.07
  • Accepted : 2015.11.09
  • Published : 2016.01.27

Abstract

The graphene oxides (GOs) were tested as a fluorescent quencher in the field of DNA-diagnostics. The various suspensions of GO nanoplates were prepared by changing the synthesis conditions. The suspensions were stable for at least 6 weeks by differing degrees of functionalization of various oxygen-containing groups of atoms. Depending on the properties of GO nanoplates, their fluorescent quenching abilities, which were determined by the amount of the tagged immobilized oligonucleotide, were also changed. GO suspension synthesized at $75^{\circ}C$ of reaction mixture showed the fluorescent quenching of 16.39 nmol/mg, which would be a potential substitution of molecular fluorescent quencher in test-systems for DNA-diagnostics.

Keywords

graphene oxide;DNA diagnostics;fluorescent quencher

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Cited by

  1. Synthesis of Luminescent N-Doped Carbon Dots by Hydrothermal Treatment pp.03701972, 2017, https://doi.org/10.1002/pssb.201700222

Acknowledgement

Grant : Research and development on priority directions of scientific-technological complex of Russia for 2014-2020 years

Supported by : Ministry of Education