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Effect of Extraction Conditions of Green Tea on Antioxidant Activity and EGCG Content: Optimization using Response Surface Methodology

  • Kim, Mun Jun (College of Pharmacy, Chungbuk National University) ;
  • Ahn, Jong Hoon (College of Pharmacy, Chungbuk National University) ;
  • Kim, Seon Beom (College of Pharmacy, Chungbuk National University) ;
  • Jo, Yang Hee (College of Pharmacy, Chungbuk National University) ;
  • Liu, Qing (College of Pharmacy, Chungbuk National University) ;
  • Hwang, Bang Yeon (College of Pharmacy, Chungbuk National University) ;
  • Lee, Mi Kyeong (College of Pharmacy, Chungbuk National University)
  • Received : 2016.06.30
  • Accepted : 2016.08.20
  • Published : 2016.12.31

Abstract

Green tea, the leaves of Camellia sinsneis (Theaceae), is generally acknowledged as the most consumed beverage with multiple pharmacological functions including antioxidant activity. This study was performed to analyze the effect of extraction conditions of green tea on its antioxidant effects using DPPH assay. Three extraction factors such as extraction solvent (EtOH, 0 - 100%), extraction time (3 - 15 min) and extraction temperature ($10-70^{\circ}C$) were analyzed and optimized extraction condition for antioxidant activity of green tea extract (GTE) was determined using response surface methodology with three-level-three-factor Box-Behnken design (BBD). Regression analysis showed a good fit of data and the optimal conditions of extraction were found to be 57.7% EtOH, 15 min and $70^{\circ}C$. Under this condition, antioxidant activity of experimental data was 88.4% which was almost fit to the ideal value of 88.6%. As epigallocatechin gallate (EGCG) is known for the major ingredient for antioxidant activity of green tea, we investigated the effect of EGCG on antioxidant activity of GTE. EGCG showed antioxidant activity with the $IC_{50}$ value of $4.2{\mu}g/ml$ and a positive correlation was observed between EGCG content and the antioxidant activity of GTE with $R^2=0.7134$. Interestingly, however, GTE with 50 - 70% antioxidant activity contain less than $1.0{\mu}g/ml$ of EGCG, which is much lower than $IC_{50}$ value of EGCG. Therefore, we suppose that EGCG together with other constituents contribute to antioxidant activity of GTE. Taken together, these results suggest that green tea is more beneficial than EGCG alone for antioxidant ability and optimal extraction condition of green tea will be useful for the development of food and pharmaceutical applications

Acknowledgement

Supported by : Chungbuk National University

References

  1. Zhang, L.; Pang, E.; Loo, R. R.; Rao, J.; Go, V. L.; Loo, J. A.; Lu, Q. Y. Proteomics 2011, 11, 4638-4647. https://doi.org/10.1002/pmic.201100242
  2. Bornhoeft, J.; Castaneda, D.; Nemoseck, T.; Wang, P.; Henning, S. M.; Hong, M. Y. J. Med. Food 2012, 15, 726-732. https://doi.org/10.1089/jmf.2011.0258
  3. Rickman, C.; Lyer, A.; Chan, V.; Brown, L. Cur. Pharm. Biotechnol. 2010, 11, 881-886. https://doi.org/10.2174/138920110793261980
  4. Ramadan, G.; El-Beih, N. M.; Abd El-Ghffar, E. A. Br. J. Nutr. 2009, 102, 1611-1619. https://doi.org/10.1017/S000711450999208X
  5. Du, G. J.; Zhang, Z.; Wen, X.D.; Yu, C.; Calway, T.; Yuan, C. S.; Wang, C. Z. Nutrients 2012, 4, 1679-1691. https://doi.org/10.3390/nu4111679
  6. Zhong, Y.; Chiou, Y. S.; Pan, M. H.; Shahidi, F. Food Chem. 2012, 134, 742-748. https://doi.org/10.1016/j.foodchem.2012.02.172
  7. Steinmann, J.; Buer, J.; Pietschmann, T.; Steinmann, E. Br. J. Pharmacol. 2013, 168, 1059-1073. https://doi.org/10.1111/bph.12009
  8. Betteridge, D. J. Metabolism 2000, 49, 3-8.
  9. Yoshikawa, T.; Naito, Y. J. Japan Med. Ass. 2002, 45, 271-276.
  10. Gostner, J. M.; Becker, K.; Ueberall, F.; Fuchs, D. Food Chem. Toxicol. 2015, 80, 72-79. https://doi.org/10.1016/j.fct.2015.02.012
  11. Garcia-Nino, W. R.; Zazueta, C. Pharmacol. Res. 2015, 97, 84-103. https://doi.org/10.1016/j.phrs.2015.04.008
  12. de Oliveira, C. C.; de Araujo C. V. M.; Ares, G.; Granato, D. Crit. Rev. Food Sci. Nutr. 2015, 55, 1456-1473. https://doi.org/10.1080/10408398.2012.750233
  13. Legeay, S.; Rodier, M.; Fillon, L.; Faure, S.; Clere, N. Nutrients 2015, 7, 5443-5468. https://doi.org/10.3390/nu7075230
  14. Tsai, C. F.; Hsu, Y. W.; Ting, H. C.; Huang, C. F.; Yen, C. C. Food Chem. 2013, 136, 1337-1344. https://doi.org/10.1016/j.foodchem.2012.09.063
  15. Zhang, W. M.; Huang, W.Y.; Chen, W. X.; Han, L.; Zhang, H. D. Molecules 2014, 19, 16416-16427. https://doi.org/10.3390/molecules191016416
  16. Jeong, J. Y.; Jo, Y. H.; Kim, S. B.; Liu, Q.; Lee, J. W.; Mo, E. J.; Lee, K. Y.; Hwang, B. Y.; Lee, M. K. Bioorg. Med. Chem. Lett. 2015, 25, 2269-2274. https://doi.org/10.1016/j.bmcl.2015.04.045
  17. Jeong, J. Y.; Jo, Y. H.; Lee, K. Y.; Do, S. G.; Hwang, B. Y.; Lee, M. K. Bioorg. Med. Chem. Lett. 2014, 24, 2329-2333. https://doi.org/10.1016/j.bmcl.2014.03.067
  18. Bezerra, M. A.; Santelli, R. E.; Oliveira, E. P.; Villar, L. S.; Escaleira, L. A. Talanta 2008, 76, 965-977. https://doi.org/10.1016/j.talanta.2008.05.019
  19. Ferreira, S. L. C.; Bruns, R. E.; Ferreira, H. S.; Matos, G. D.; David, J. M.; Brandao, G. C.; Da Silva, E. G. P.; Portugal, L. A.; dos Reis, P. S.; Souza, A. S.; dos Santos, W. N. L. Anal. Chim. Acta 2007, 597, 179-186. https://doi.org/10.1016/j.aca.2007.07.011
  20. Kim, S. B.; Jo, Y. H.; Liu, Q.; Ahn, J. H.; Hong, I. P.; Han, S. M.; Hwang, B. Y.; Lee, M. K. Molecules 2015, 20, 19764-19774. https://doi.org/10.3390/molecules201119656
  21. Lambert, J. D.; Elias, R. J. Arch. Biochem. Biophys. 2010, 501, 65-72. https://doi.org/10.1016/j.abb.2010.06.013
  22. Senthil K. V.; Arulmathi, K.; Srividhya, R.; Kalaiselvi, P. Exp. Gerontol. 2008, 43, 176-18. https://doi.org/10.1016/j.exger.2007.10.017
  23. Jovanovic, S. V.; Hara, Y.; Steenken, S.; Simic, M. G. J. Am. Chem. Soc. 1995, 117, 9881-9888. https://doi.org/10.1021/ja00144a014