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Isolation and Characterization of Soil Bacteria Degrading a Fungicide Defenoconazole

살균제 디페노코나졸 분해 세균 분리 및 특성 분석

  • Ahn, Jae-Hyung (Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration (RDA)) ;
  • Ro, Yu-Mi (Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration (RDA)) ;
  • Lee, Gwan-Hyeong (Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration (RDA)) ;
  • Park, InCheol (Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration (RDA)) ;
  • Kim, Wan-Gyu (Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration (RDA)) ;
  • Han, Byeong-Hak (Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration (RDA)) ;
  • You, Jaehong (Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration (RDA))
  • 안재형 (국립농업과학원 농업미생물과) ;
  • 노유미 (국립농업과학원 농업미생물과) ;
  • 이관형 (국립농업과학원 농업미생물과) ;
  • 박인철 (국립농업과학원 농업미생물과) ;
  • 김완규 (국립농업과학원 농업미생물과) ;
  • 한병학 (국립농업과학원 농업미생물과) ;
  • 유재홍 (국립농업과학원 농업미생물과)
  • Received : 2016.10.22
  • Accepted : 2016.11.29
  • Published : 2016.12.31

Abstract

Triazole fungicides occupy an important portion in the global fungicide market and are relatively persistent in soil compared to the other fungicides, suggesting possible adverse effects of the fungicides on human health and environment. In this study, we tried to isolate microorganisms from orchard soils, which can decompose the triazole fungicides, tebuconazole, fluquinconazole, and difenoconazole. Only difenoconazole was completely degraded in the enrichment culture, from which several difenoconazole-degrading bacteria were isolated. They showed the same rep-PCR pattern thus only one strain, C8-2, was further studied. The strain was identified as Sphingomonas sp. C8-2 based on its 16S rRNA gene sequence and decomposed 100 mg/L of difenoconazole in a minimum medium to an unknown metabolite with a molecular weight of 296 within 24 hours. The inhibition effect of the metabolite against representative soil microorganisms significantly decreased compared to that of difenoconazole thus the bacterial strain is expected to be used for the detoxification of difenoconazole in soil and crop.

Acknowledgement

Supported by : 국립농업과학원

References

  1. Badawi, N., A. E. Rosenbom, A. M. Jensen and S. R. Sorensen (2016) Degradation and sorption of the fungicide tebuconazole in soils from golf greens. Environ. Pollut. 219:368-378. https://doi.org/10.1016/j.envpol.2016.10.045
  2. Bromilow, R. H., A. A. Evans and P. H. Nicholls (1999a) Factors affecting degradation rates of five triazole fungicides in two soil types: 1. Laboratory incubations. Psetic. Sci. 55:1129-1134.
  3. Bromilow, R. H., A. A. Evans and P. H. Nicholls (1999b) Factors affecting degradation rates of five triazole fungicides in two soil types: 2. Field studies. Psetic. Sci. 55: 1135-1142. https://doi.org/10.1002/(SICI)1096-9063(199912)55:12<1135::AID-PS73>3.0.CO;2-1
  4. Fenner, K., S. Canonica, L. P. Wackett and M. Elsner (2013) Evaluating pesticide degradation in the environment: blind spots and emerging opportunities. Science 341:752-758. https://doi.org/10.1126/science.1236281
  5. Jarocki, P., M. Podle ny, E. Komo -Janczara, J. Kucharska, A. Glibowska and Z. Targo ski (2016) Comparison of various molecular methods for rapid differentiation of intestinal bifidobacteria at the species, subspecies and strain level. BMC Microbiol. 16:159. https://doi.org/10.1186/s12866-016-0779-3
  6. KCPA (2016) Agrochemical year book. Korea Crop Protection Association, Seoul, Korea.
  7. Kim, I. S., J. H. Shim and Y. T. Suh (2003) Laboratory studies on formation of bound residues and degradation of propiconazole in soils. Pest Manag. Sci. 59:324-330. https://doi.org/10.1002/ps.642
  8. Miller, T. R., A. L. Delcher, S. L. Salzberg, E. Saunders, J. C. Detter and R. U. Halden (2010) Genome sequence of the dioxin-mineralizing bacterium Sphingomonas wittichii RW1. J. Bacteriol. 192:6101-6102. https://doi.org/10.1128/JB.01030-10
  9. Mu, X., T. Chai, K. Wang, L. Zhu, Y. Huang, G. Shen, Y. Li, X. Li and C. Wang (2016) The developmental effect of difenoconazole on zebrafish embryos: A mechanism research. Environ. Pollut. 212: 18-26. https://doi.org/10.1016/j.envpol.2016.01.035
  10. Research and markets (2014) Global fungicides market (type, (http://www.researchandmarkets.com/research/7dtjwc/global_fungicides)
  11. Sehnem, N. T., P. Souza-Cruz, C. Peralba Mdo and M. A. Ayub (2010) Biodegradation of tebuconazole by bacteria isolated from contaminated soils. J. Environ. Sci. Heal. B 45:67-72.
  12. Taxvig, C., U. Hass, M. Axelstad, M. Dalgaard, J. Boberg, H. R. Andeasen and A. M. Vinggaard (2007) Endocrinedisrupting activities in vivo of the fungicides tebuconazole and epoxiconazole. Toxicol. Sci. 100:464-473. https://doi.org/10.1093/toxsci/kfm227
  13. Zhou, J., J. Zhang, F. Li and J. Liu (2016) Triazole fungicide tebuconazole disrupts human placental trophoblast cell functions. J. Hazard. Mater. 308:294-302. https://doi.org/10.1016/j.jhazmat.2016.01.055