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Isolation and Characterization of Bacillus Species Having Antifungal Activity Against Pathogens of Ginseng Damping Off

인삼모잘록병원균에 항균활성을 갖는 Bacillus 균의 분리 및 특성조사

  • Park, Kyeong Hun (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA) ;
  • Park, Hong Woo (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA) ;
  • Lee, Seong Woo (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA) ;
  • Lee, Seung Ho (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA) ;
  • Myung, Kyung Sun (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA) ;
  • Lee, Sang Yeob (Agricultural microbiology division, National Institute of Agricultural Science, RDA) ;
  • Song, Jaekyeong (Agricultural microbiology division, National Institute of Agricultural Science, RDA) ;
  • Kim, Young Tak (Seed Certification Team, Quality Assurance Division, Nongwoo Bio CO., LTD.) ;
  • Park, Kyoung Soo (Seed Certification Team, Quality Assurance Division, Nongwoo Bio CO., LTD.) ;
  • Kim, Young Ock (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA)
  • 박경훈 (국립원예특작과학원 인삼특작부) ;
  • 박홍우 (국립원예특작과학원 인삼특작부) ;
  • 이성우 (국립원예특작과학원 인삼특작부) ;
  • 이승호 (국립원예특작과학원 인삼특작부) ;
  • 명경선 (국립원예특작과학원 인삼특작부) ;
  • 이상엽 (국립농업과학원 농업미생물과) ;
  • 송재경 (국립농업과학원 농업미생물과) ;
  • 김영탁 (농우바이오 종자검정팀) ;
  • 박경수 (농우바이오 종자검정팀) ;
  • 김영옥 (국립원예특작과학원 인삼특작부)
  • Received : 2016.10.24
  • Accepted : 2016.11.23
  • Published : 2016.12.31

Abstract

This study was performed to select potentially available biological control agent from soil bacteria for prevention of ginseng damping off. More than five hundred strains were isolated from ginseng rhizosphere soil. By testing antifungal activity, we have selected three soil bacteria strains and their ability to produce antibiotics and lytic enzymes such as cellulase, protease and pectate lyase was examined. Also, the presence of genes for biosynthesis of lipopeptide such as fengycin, bacillomycin D, surfactin, iturin A, and zwittermicin A was investigated in selected strains. All three strains produced cellulase, protease, and xylanase. Moreover, these strains had gene for biosynthesis of bacillomycin D, surfactin, and iturin A. ES1 and ES3 strains were identified Bacillus methylotrophucus and ES2 was confirmed Bacillus amyloliquefaciens using phylogenetic analysis on the basis of 16S rRNA gene sequences. In field test, control value of ES1, ES2 and ES3 treatment was 32.4%, 46.8% and 36.7%, respectively. This results indicate that antagonistic microbes with high ability of antifungal and lytic enzyme activity can be used as a useful biological control agent to control ginseng damping off.

Acknowledgement

Supported by : 농촌진흥청

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