DOI QR코드

DOI QR Code

Lysimachia foenum-graecum Herba Extract, a Novel Biopesticide, Inhibits ABC Transporter Genes and Mycelial Growth of Magnaporthe oryzae

  • Received : 2015.08.13
  • Accepted : 2015.11.02
  • Published : 2016.02.01

Abstract

To identify a novel biopesticide controlling rice blast disease caused by Magnaporthe oryzae, 700 plant extracts were evaluated for their inhibitory effects on mycelial growth of M. oryzae. The L. foenum-graecum Herba extract showed the lowest inhibition concentration ($IC_{50}$) of $39.28{\mu}g/ml$, which is lower than the $IC_{50}$ of blasticidin S ($63.06{\mu}g/ml$), a conventional fungicide for rice blast disease. When treatments were combined, the $IC_{50}$ of blasticidin S was dramatically reduced to $10.67{\mu}g/ml$. Since ABC transporter genes are involved in fungicide resistance of many organisms, we performed RT-PCR to investigate the transcriptional changes of 40 ABC transporter family genes of M. oryzae treated with the plant extract, blasticidin S, and tetrandrine, a recognized ABC transporter inhibitor. Four ABC transporter genes were prominently activated by blasticidin S treatment, but were suppressed by combinational treatment of blasticidin S with the plant extract, or with tetrandrine that didn't show cellular toxicity by itself in this study. Mycelial death was detected via confocal microscopy at 24 h after plant extract treatment. Finally, subsequent rice field study revealed that the plant extract had high control efficacy of 63.3% and should be considered a biopesticide for rice blast disease. These results showed that extract of L. foenum graecum Herba suppresses M. oryzae ABC transporter genes inducing mycelial death and therefore may be a potent novel biopesticide.

Keywords

ABC transporter gene;biopesticide;blasticidin S;fungicide resistance;Lysimachia foenum-graecum Herba;Magnaporthe oryzae;plant extract;rice blast disease

References

  1. Lee, S. E., Ju, E. M. and Kim, J. H. 2002. Antioxidant activity of extracts from Euryale ferox seed. Exp. Mol. Medicine 34:100-106. https://doi.org/10.1038/emm.2002.15
  2. Li, R. Y., Wu, X. M., Yin, X. H., Liang, J. N. and Li, M. 2014. The natural product citral can cause significant damage to the hyphal cell walls of Magnaporthe grisea. Molecules 19:10279-10290. https://doi.org/10.3390/molecules190710279
  3. Szakacs, G., Paterson, J. K., Ludwig, J. A., Booth-Genthe, C. and Gottsman, M. M. 2006. Targeting multidrug resistance in cancer. Nat. Rev. Drug Discovery 5:219-234. https://doi.org/10.1038/nrd1984
  4. Thakur, S., Jha, S., Roy-Barman, S. and Chattoo, B. 2009. Genomic resources of Magnaporthe oryzae (GROMO): A comprehensive and integrated database on rice blast fungus. BMC Genomics 10:316. https://doi.org/10.1186/1471-2164-10-316
  5. Zolnerciks, J. K. and Andress, E. J. 2011. Structure of ABC transporters. Essays Biochem. 50:43-61. https://doi.org/10.1042/bse0500043
  6. Abouzeed, Y. M., Baucheron, S. and Cloeckaert, A. 2008. ramB mutations involved in efflux-mediated multidrug resistance in Salmonella enterica serovar Typhimurium. Antimicrob. Agents Chemother. 52:2428-2434. https://doi.org/10.1128/AAC.00084-08
  7. Colabufo, N. A., Berardi, F., Cantore, M., Contino, M., Inglese, C., Niso, M. and Perrone, R. 2010. Perspectives of P-glycoprotein modulating agents in oncology and neurodegenerative diseases. pharmaceutical, biological, and diagnostic potentials. J. Med. Chem. 53:1883-1897. https://doi.org/10.1021/jm900743c
  8. Cowman, A. F., Karcz, S., Galatis, D. and Culvenor, J. G. 1991. A P-glycoprotein homologue of plasmodium falciparum is localized on the digestive vacuole. J. Cell Biol. 113:1033-1042. https://doi.org/10.1083/jcb.113.5.1033
  9. Dean, R. A., Talbot, N. J., Ebbole, D. J., Farman, M. L., Mitchell, T. K., Orbach, M. J., Thon, M., Kulkarni, R., Xu, J. R., Pan, H., Read, N. D., Lee, Y. H., Carbone, I., Brown, D., Oh, Y. Y., Donofrio, N., Jeong, J. S., Soanes, D. M., Djonovic, S., Kolomiets, E., Rehmeyer, C., Li, W., Harding, M., Kim, S., Lebrun, M. H., Bohnert, H., Coughlan, S., Butler, J., Calvo, S., Ma, L. J., Nicol, R., Purcell, S., Nusbaum, C., Galagan, J. E. and Birren, B. W. 2005. The genome sequence of the rice blast fungus Magnaporthe grisea. Nature 434:980-986. https://doi.org/10.1038/nature03449
  10. Dixon, R. A. and Lamb, C. J. 1990. Molecular communication in interactions between plants and microbial pathogens. Annu. Rev. Plant Physiol. 14:339-367.
  11. Dixon, R. A., Harrison, M. J. and Lamb, C. J. 1994. Early events in the activation of plant defense responses. Annu. Rev. Phytopathol. 32:479-501. https://doi.org/10.1146/annurev.py.32.090194.002403
  12. Gulshan, K. and Moye-Rowley, W. S. 2007. Multidrug resistance in fungi. Eukaryotic Cell 6:1933-1942. https://doi.org/10.1128/EC.00254-07
  13. Jones, P. M. and George, A. M. 2004. The ABC transporter structure and mechanism: perspectives on recent research. Cell. Mol. Life Sci. 61:682-699. https://doi.org/10.1007/s00018-003-3336-9
  14. Kim, Y. N., Kim, J. S., Kim, S. Y., Kim, J.-H., Lee, J.-H. and Choi, W. B. 2010. Prediction and annotation of ABC transporter genes from Magnaporthe oryzae genome sequence. J. Life Sci. 20:176-182. https://doi.org/10.5352/JLS.2010.20.2.176
  15. Kim, S. Y., Park, S.-Y., Kim, H. J., Kim, D. Y., Lee, S.-W., Kim, H. T., Lee, J. H. and Choi, W. B. 2014. A paper on ABC transporter of plant fungal pathogen should be also cited. Isolation and characterization of the Colletotrichum acutatum ABC transporter CaABC1. Plant Pathol. J. 30:375-383. https://doi.org/10.5423/PPJ.OA.08.2014.0077
  16. Kim, Y. N., Park, S.-Y., Kim, D. Y., Choi, J. Y., Lee, Y.-H., Lee, J.-H. and Choi, W. B. 2013. Genome-scale analysis of ABC transporter genes and characterization of the ABCC type transporter genes in Magnaporthe oryzae. Genomics 101: 354-361. https://doi.org/10.1016/j.ygeno.2013.04.003
  17. Kubo, I. and Lee, S. H. 1998. Potentiation of antifungal activity of sorbic acid. J. Agric. Food Chem. 46:4052-4055. https://doi.org/10.1021/jf980174o
  18. Lee, Y.-J., Yamamoto, K., Hamamoto, H., Nakaune, R. and Hibi, T. 2005. A novel ABC transporter gene ABC2 involved in multidrug susceptibility but not pathogenicity in rice blast fungus, Magnaporthe grisea. Pesti. Biochem. Physiol. 81:13- 23. https://doi.org/10.1016/j.pestbp.2004.07.007
  19. Li, G. and Wang, Y. Q. 2006. Progress in the research on the ABCA gene family of vertebrates. Yi Chuan 28:1015-1022.
  20. Mohandas, N., Winardi, R., Knowles, D., Leung, A., Parra, M. George, E., Conboy, J. and Chasis, J. 1992. Molecular basis for membrane rigidity of domain hereditary ovalocytosis. a novel mechanism involving the cytoplasmic of band 3. J. Clin. Invest. 89:686-692. https://doi.org/10.1172/JCI115636
  21. Osbourn, A. E. 1996. Preformed antimicrobial compounds and plant defense against fungal attack. Plant Cell 8:1821-1831. https://doi.org/10.1105/tpc.8.10.1821
  22. Park, I. K., Kim, J. H., Lee, Y. S. and Shin, S. C. January-March. 2008. In vivo fungicidal activity of medicinal plant extracts against six phytopathogenic fungi. Int. J. Pest Management 54:63-68. https://doi.org/10.1080/09670870701549665
  23. Ponte-Sucre, A. 2009. ABC Transporters in microorganisms: Research, Innovation and Value as Targets against Drug Resistance. Caister Academic Press. ISBN 978-1-904455-49-3.
  24. Riordan, J. R. and Ling, V. 1979. Purification of P-glycoprotein from plasma membrane vesicles of chinese hamster ovary cell mutants with reduced colchicine permeability. J. Biol. Chem. 254:12701-12705.
  25. Rodrigues, L., Machado, D., Couto, I., Amaral, L. and Viveiros, M. 2012. Contribution of efflux activity to isoniazid resistance in the mycobacterium tuberculosis complex. Infect. Genet. Evol. 12:695-700. https://doi.org/10.1016/j.meegid.2011.08.009
  26. Sesan, T. E., Enache, E., Iacomi, B. M., Oprea, M., Oancea, F. and Iacomi, C. 2015. Antifungal activity of some plant extract against Botrytis cinerea Pers. In the blackcurrant crop (Ribes nigrum L.). Acta Sci. Pol., Hortorum Cultus 14:29-43.
  27. Sharom, F. J. 2008. ABC multidrug transporters: structure, function and role in chemoresistance. Pharmacogenomics 9:105- 127. https://doi.org/10.2217/14622416.9.1.105
  28. Stavrovskaya, A. A. 2000. Cellular mechanisms of multidrug resistance of tumor cells. Biochemistry (Mosc) 65:95-106.

Acknowledgement

Supported by : Korea Small and Medium Business Administration